A novel method to crystallizing proteins by using two-dimensional organic-inorganichybrid material
| Project/Area Number |
24657068
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
YAO Min 北海道大学, 先端生命科学研究科(研究院), 教授 (40311518)
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| Research Collaborator |
YAO Ken 中国上海大学, 理学院, 教授
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | タンパク質結晶化 / 規則的二次元分子 / CPAPhS / 結晶核形成 / X線結晶構造解析 / 分子材料 / 二次元分子空間 / 構造解析 / 結晶化 / 二次元分子材料 |
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| Outline of Final Research Achievements |
In order to increase the probability of forming crystal kernels for proteins, we develop a novel method by using chemical material. In this method, we use a new material which is regular and functional two-dimensional organic-inorganic hybrid material. We have got succeeded to bind peptides on it in regular arrangement and link them to proteins. We tried to crystallize Trypsin and Rpf2-Rrs1 complex by using this new material with regular peptides, and found a way to develop different new materials further.
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Report
(4 results)
Research Products
(8 results)
