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A novel method to crystallizing proteins by using two-dimensional organic-inorganichybrid material

Research Project

Project/Area Number 24657068
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionHokkaido University

Principal Investigator

YAO Min  北海道大学, 先端生命科学研究科(研究院), 教授 (40311518)

Research Collaborator YAO Ken  中国上海大学, 理学院, 教授
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsタンパク質結晶化 / 規則的二次元分子 / CPAPhS / 結晶核形成 / X線結晶構造解析 / 分子材料 / 二次元分子空間 / 構造解析 / 結晶化 / 二次元分子材料
Outline of Final Research Achievements

In order to increase the probability of forming crystal kernels for proteins, we develop a novel method by using chemical material. In this method, we use a new material which is regular and functional two-dimensional organic-inorganic hybrid material. We have got succeeded to bind peptides on it in regular arrangement and link them to proteins. We tried to crystallize Trypsin and Rpf2-Rrs1 complex by using this new material with regular peptides, and found a way to develop different new materials further.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (2 results) Remarks (2 results)

  • [Journal Article] Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis2015

    • Author(s)
      Nozomi Asano, Koji Kato, Akiyoshi Nakamura, Keisuke Komoda, Isao Tanaka, Min Yao
    • Journal Title

      Nucleic Acids Research

      Volume: 印刷中 Issue: 9 Pages: 4746-4757

    • DOI

      10.1093/nar/gkv305

    • NAID

      120005649121

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of ribosome assembly factors: the Rpf2-Rrs1 complex2014

    • Author(s)
      Nozomi Asano, Akiyoshi Nakamura, Keisuke Komoda, Koji Kato, Isao Tanaka and Min Yao
    • Journal Title

      Acta Crystallographica Section F

      Volume: 70 Issue: 12 Pages: 1649-1652

    • DOI

      10.1107/s2053230x14024182

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of alpha-glucosidase HaG from Halomonas sp. strain H112014

    • Author(s)
      X. Shen, W. Saburi, Z.-Q. Gai, K. Komoda, J. Yu, T. Ojima-Kato, Y. Kido, H. Matsui, H. Mori and M. Yao
    • Journal Title

      Acta Crystallographica Section F

      Volume: F70 Issue: 4 Pages: 464-466

    • DOI

      10.1107/s2053230x14001940

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional two-dimensional organic-inorganic hybrid materials with regular peptides2013

    • Author(s)
      Xue Shao
    • Journal Title

      Colloids and Surfaces A: Physicochem. Eng. Aspects

      Volume: 424 Pages: 66-73

    • DOI

      10.1016/j.colsurfa.2013.02.011

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 真核生物リボソーム生合成関連因子Rpf2とRrs1の構造機能解析2015

    • Author(s)
      朝野希美,加藤公児,中村彰良,薦田圭介,田中勲,姚閔
    • Organizer
      第3回RIBOSOME MEETING
    • Place of Presentation
      ANAホリデイ・インリゾート宮崎(宮崎市)
    • Year and Date
      2015-03-17 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] リボソーム成熟に必須なタンパク質複合体の結晶構造解析へのチャレンジ2014

    • Author(s)
      朝野希美,中村彰良,薦田圭介,加藤公児,田中勲,姚閔
    • Organizer
      平成26年度日本結晶学会年会及び総会
    • Place of Presentation
      東京大学農学部(東京都文京区)
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Annual Research Report
  • [Remarks] 北海道大学 X線構造生物学研究室 ホームページ

    • URL

      http://altair.sci.hokudai.ac.jp/g6/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 北海道大学 X線構造生物学研究室 ホームページ

    • URL

      http://altair.sci.hokudai.ac.jp/g6/

    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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