Development of Novel monitoring method of autophagy

• Project/Area Number: 24657089
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: NODA Takeshi 大阪大学, 歯学研究科(研究院), 教授 (00290908)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: オートファジー

Research Abstract

Autophagy is a process that the cell degrades its own components and reutilize them. Several environmental stress such as starvation induces autophagy and proceeds gradually and finally it is terminated. As the method for monitoring autophagy, I previously developed ALP assay in the yeast and the methods is still widely used. ALP assay enables quantitative measurement of autophagy based on the enzymatic activity of alkaline phosphatase. This time, I tried to developed similar method to ALP assay in mammalian cells. I generated various version of acid phosphatase to test if their activity is correlated with the progression of autophagy, but finally successful one could not be obtained. On the other hand, we succeeded in the development of genome wide ALP assay.

Research Products

• [Journal Article] Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin (2013)
  • Author(s): Fujita N, Morita E, (equally contributed) Itoh T, Tanaka A, Nakaoka M, Osada Y, Umemoto T, Saitoh T, Nakatogawa H, Kobayashi S, Haraguchi T, Guan JL, Iwai K, Tokunaga F, Saito K, Ishibashi K, Akira S, Fukuda M, Noda T, Yoshimori T
  • Journal Title: The Journal of Cell Biology Volume: 203(1) Pages: 115-128
• [Journal Article] Autophagy sequesters damaged lysosomes to control lysosomal biogenesis and kidney injury (2013)
  • Author(s): Maejima I, Takahashi A, Omori H, Kimura T, Takabatake Y, Saitoh T, Yamamoto A, Hamasaki M, Noda T, Isaka Y, Yoshimori T
  • Journal Title: EMBO journal Pages: 2336-2347
• [Journal Article] TRAPPIII is responsible for the vesicular transport from early endosomes to the Golgi apparatus that facilitates Atg9 cycling in autophagy (2013)
  • Author(s): Shirahama-Noda K, Kira S, Yoshimori T, Noda T
  • Journal Title: J Cell Sci. Volume: 126 Pages: 4963-73
  • DOI: 10.1242/jcs.131318
  • Peer Reviewed / Open Access
• [Journal Article] Three-Axis Model for Atg Recruitment in Autophagy against Salmonella (2012)
  • Author(s): Noda T, Kageyama S, Fujita N, Yoshimori T.
  • Journal Title: Int J Cell Biol. Volume: 2012 Pages: 1-6
  • DOI: 10.1155/2012/389562
  • Peer Reviewed
• [Journal Article] TRAPPIII is responsible for the vesicular transport from early endosomes to the Golgi apparatus that facilitates Atg9 cycling in autophagy
  • Author(s): Shirahama-Noda K, Kira S, Yoshimori T, Noda T
  • Journal Title: Journal of Cell Science Volume: 126 Pages: 4963-4973
• [Presentation] 長時間飢餓条件下におけるオートファジー停止機構の研究 (2013)
  • Author(s): 野口雅史、吉良新太郎、吉森保、野田健司
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-03
• [Presentation] オートファジーとTORC1キナーゼの制御機構におけるNpr2の役割 (2013)
  • Author(s): 吉良新太郎、田端桂介、吉森保、野田健司
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2013-06-19
• [Presentation] Critical role of PtdIns3P turn over in autophagy regulation (2013)
  • Author(s): T NODA
  • Organizer: The 90^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Tower Hall Funahori, Tokyo
  • Year and Date: 2013-03-29
• [Presentation] TRAPPIII is responsible for the vesicular transport from early endosomes to the Golgi apparatus that facilitates Atg9 cycling in autophagy (2013)
  • Author(s): Shirahama-Noda K, Kira S, Yoshimori T, Noda T
  • Organizer: Gordon research conference molecular membrane biology
  • Place of Presentation: アメリカニューハンプシャーProctor Academy Andover
• [Presentation] Regulatory Mechanism of Autophagy in yeast (2013)
  • Author(s): T NODA
  • Organizer: 3rd China-Japan symposium on autophagy
  • Place of Presentation: Dunhuang China
• [Presentation] TRAPPIII is responsible for vesicular transport from early endosomes to Golgi, facilitating Atg9 cycling in autophagy (2013)
  • Author(s): 野田健司
  • Organizer: 第7回オートファジー研究会
  • Place of Presentation: 掛川
• [Presentation] Critical role of PtdIns3P turn over in autophagy regulation (2013)
  • Author(s): Takeshi NODA
  • Organizer: 日本生理学会
  • Place of Presentation: 船掘ホール 東京
  • Invited
• [Presentation] オートファジー制御機構の包括的理解への試み (2012)
  • Author(s): 吉良 新太郎 野田 佳苗 吉森 保 野田 健司
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Invited
• [Remarks]
  • URL: http://web.dent.osaka-u.ac.jp/~cfos/index.html