Epigenetic regulation of circadian clock development during ES cell differentiation

• Project/Area Number: 24657096
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TSUCHIYA Yoshiki 京都府立医科大学, 医学(系)研究科(研究院), 講師 (30456777)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 概日リズム / ES細胞

Research Abstract

I demonstrated that the methylation state of histone H3 at the promoter region of Per2 gene in mouse ES cells is different between before and after ES cell differentiation. I also found that histone deacetylase activity is involved in the repression of Per2 expression in undifferentiated ES cells. These results suggest that epigenetic regulation of Per2 expression underlies development of the circadian clock during ES cell differentiation.