A development of novel fluorescence microscopy toward 20 color simultaneous imaging.

Research Project

Project/Area Number	24657112
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Biophysics
Research Institution	Institute of Physical and Chemical Research
Principal Investigator	OZAKI Yuichi 独立行政法人理化学研究所, 生命システム研究センター, 研究員 (70345114)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	バイオイメージング
Research Abstract	In this study, I have developed a novel polychromatic fluorescence microscopy to overcome the difficulty of linear-unmixing technique that prerequisite reference spectra. I have developed a blind unmixing algorithm, and shown that the algorithm can decompose all fluorescence spectra accurately from a fading sequence of spectral images. I also developed a high speed spectral imaging microscope system on the basis of laser scanning microscope.

Report

(3 results)