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A development of novel fluorescence microscopy toward 20 color simultaneous imaging.

Research Project

Project/Area Number 24657112
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

OZAKI Yuichi  独立行政法人理化学研究所, 生命システム研究センター, 研究員 (70345114)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsバイオイメージング
Research Abstract

In this study, I have developed a novel polychromatic fluorescence microscopy to overcome the difficulty of linear-unmixing technique that prerequisite reference spectra. I have developed a blind unmixing algorithm, and shown that the algorithm can decompose all fluorescence spectra accurately from a fading sequence of spectral images. I also developed a high speed spectral imaging microscope system on the basis of laser scanning microscope.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2012

All Presentation (2 results) Patent(Industrial Property Rights) (2 results)

  • [Presentation] 超多重蛍光顕微鏡法の開発2012

    • Author(s)
      尾崎裕一
    • Organizer
      定量生物の会第5回年会
    • Place of Presentation
      東京大学生産技術研究所
    • Related Report
      2013 Final Research Report
  • [Presentation] 超多重蛍光顕微鏡法の開発2012

    • Author(s)
      尾崎 裕一
    • Organizer
      量生物の会第5回年会
    • Place of Presentation
      東京大学生産技術研究所
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 多重蛍光画像の画像解析のための装置,システム,方法,およびプログラム2012

    • Inventor(s)
      尾崎 裕一、上田 昌弘
    • Industrial Property Rights Holder
      独立行政法人理化学研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-084643
    • Filing Date
      2012-04-03
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 多重蛍光画像の画像解析のための装置, システム, 方法, およびプログラム2012

    • Inventor(s)
      尾崎裕一、上田昌宏
    • Industrial Property Rights Holder
      独立行政法人理化学研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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