A development of novel fluorescence microscopy toward 20 color simultaneous imaging.
| Project/Area Number |
24657112
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
OZAKI Yuichi 独立行政法人理化学研究所, 生命システム研究センター, 研究員 (70345114)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | バイオイメージング |
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| Research Abstract |
In this study, I have developed a novel polychromatic fluorescence microscopy to overcome the difficulty of linear-unmixing technique that prerequisite reference spectra. I have developed a blind unmixing algorithm, and shown that the algorithm can decompose all fluorescence spectra accurately from a fading sequence of spectral images. I also developed a high speed spectral imaging microscope system on the basis of laser scanning microscope.
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Report
(3 results)
Research Products
(4 results)
