The mechanism and functional significance of the nuclear translocation of metabolic enzymes.

• Project/Area Number: 24657135
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: YONEDA YOSHIHIRO 大阪大学, 生命機能研究科, 教授 (80191667)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 代謝酵素 / 核輸送 / importin / transaldolase / 核タンパク質 / 細胞内局在 / メタボロミクス

Research Abstract

In this study, we examined the mechanism and functional significance of the nuclear translocation of transaldolase. Our results show that transaldolase possesses a nuclear localization signal (NLS) at N-terminus, and is imported into the nucleus by multi-subfamily of importin-alpha in conjunction with importin-beta. Further, our analysis revealed that the overexpression of the nuclear or cytoplasmic transaldolase differentially affects the cellular metabolism.

Research Products

• [Journal Article] The role of Importin-βs in the maintenance and lineage commitment of mouse embryonic stem cells (2014)
  • Author(s): Sangel P, Oka M, Yoneda Y.
  • Journal Title: FEBS Open Bio. Volume: 4 Issue: 1 Pages: 112-120
  • DOI: 10.1016/j.fob.2014.01.001
  • Peer Reviewed
• [Journal Article] Importin alpha subtypes determine differential transcription factor localization in embryonic stem cells maintenance (2013)
  • Author(s): Yasuhara N, Yamagishi R, Arai Y, Mehmood R, Kimoto C, Fujita T, Touma K, Kaneko A, Kamikawa Y, Moriyama T, Yanagida T, Kaneko H, Yoneda Y.
  • Journal Title: Dev. Cell Volume: 26 Issue: 2 Pages: 123-135
  • DOI: 10.1016/j.devcel.2013.06.022
  • Peer Reviewed
• [Journal Article] Quantitative regulation of nuclear pore complex components by O-GlcNAcylation (2013)
  • Author(s): Mizuguchi-Hata C, Ogawa Y, Oka M and Yoneda Y.
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research Volume: 1833 Issue: 12 Pages: 2682-2689
  • DOI: 10.1016/j.bbamcr.2013.06.008
  • Peer Reviewed
• [Journal Article] Cell Density-dependent Nuclear Accumulation of ELK3 is Involved in Suppression of PAI-1 Expression (2013)
  • Author(s): Tanaka S, Nakano K, Sekimoto T, Oka M and Yoneda Y.
  • Journal Title: Cell Structure and Function Volume: 38 Issue: 2 Pages: 145-154
  • DOI: 10.1247/csf.13007
  • NAID: 130004053896
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I (2013)
  • Author(s): Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K, Ohkawa Y
  • Journal Title: Nucleic Acids Res Volume: 41(14) Issue: 14 Pages: 7060-72
  • DOI: 10.1093/nar/gkt414
  • Peer Reviewed
• [Journal Article] Differential role for transcription factor Oct4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells (2013)
  • Author(s): Oka, M., Moriyama, T., Asally, M., Kawakami, K., and Yoneda, Y.
  • Journal Title: The Journal of Biological Chemistry Volume: 288 Issue: 21 Pages: 15085-15097
  • DOI: 10.1074/jbc.m112.448837
  • Peer Reviewed
• [Journal Article] Improvement in protocol to generate homogeneous glutamatergic neurons from mouse embryonic stem cells reduced apoptosis. (2013)
  • Author(s): Fujiki, R., et al.
  • Journal Title: Biochem Biophys Res Commun. Volume: 430 Issue: 2 Pages: 604-609
  • DOI: 10.1016/j.bbrc.2012.11.106
  • Peer Reviewed
• [Journal Article] The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza (2013)
  • Author(s): Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R, Watanebe T, Sakabe S, Daidoji T, Nakamura S, Kadowaki A, Ohto T, Nakanishi H, Taguchi R, Nakaya T, Murakami M, Yoneda Y, Arai H, Kawaoka Y, Penninger JM, Arita M, Imai Y.
  • Journal Title: Cell Volume: 153 Issue: 1 Pages: 112-125
  • DOI: 10.1016/j.cell.2013.02.027
  • Peer Reviewed
• [Journal Article] The Nuclear import factor importin  can protect against oxidative stress. (2013)
  • Author(s): Young JC, Ly-Huynh JD, Lescesen H, Miyamoto Y, Browne C, Yoneda Y, Koopman P, Loveland KL, Jans DA.
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research Volume: 1833 Issue: 10 Pages: 2348-2356
  • DOI: 10.1016/j.bbamcr.2013.06.007
  • Peer Reviewed
• [Journal Article] Downregulation of the small GTPase Ras-related nuclear protein accelerates cellular ageing. (2012)
  • Author(s): Nagai, M. and Yoneda, Y.
  • Journal Title: Biochim Biophys Acta. Volume: 1830 Issue: 3 Pages: 2813-2819
  • DOI: 10.1016/j.bbagen.2012.11.001
  • Peer Reviewed
• [Journal Article] Synchronizing nuclear import of ribosomal proteins with ribosome assembly. (2012)
  • Author(s): Kressler, D., et al.
  • Journal Title: Science Volume: 338 Issue: 6107 Pages: 667-671
  • DOI: 10.1126/science.1226960
  • Peer Reviewed
• [Journal Article] The interaction between importin-alpha and Nup153 promotes importin-alpha/beta-mediated nuclear import. (2012)
  • Author(s): Ogawa, Y., et al.
  • Journal Title: Traffic Volume: 13 Issue: 7 Pages: 934-946
  • DOI: 10.1111/j.1600-0854.2012.01367.x
  • Peer Reviewed
• [Presentation] Importin αによるヒストン結合タンパクRBBP4の核内移行 (2013)
  • Author(s): 辻井 聡, 盛山 哲嗣, 岡 正啓, 米田 悦啓
  • Organizer: 第６５回日本細胞生物学会
  • Place of Presentation: 愛知
• [Presentation] Estrogen receptor の核－細胞質間移動機構とその生理的意義の解明 (2013)
  • Author(s): 盛山 哲嗣，岡 正啓，米田 悦啓
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Importinαによるヒストン結合タンパクRBBP4の核内移行と細胞老化 (2013)
  • Author(s): 辻井 聡，盛山 哲嗣，岡 正啓，米田 悦啓
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Nucleocytoplasmic shuttling of estrogen receptor α. (2012)
  • Author(s): Moriyama T, 他
  • Organizer: 第６４回日本細胞生物学会
  • Place of Presentation: 神戸
• [Presentation] Recognition mode of basic NLS sequence by importin α1. (2012)
  • Author(s): Kimoto C, 他
  • Organizer: 第６４回日本細胞生物学会
  • Place of Presentation: 神戸
• [Presentation] Differential role for Oct4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells. (2012)
  • Author(s): Oka M, 他
  • Organizer: 第６４回日本細胞生物学会
  • Place of Presentation: 神戸
• [Presentation] 核―細胞質間タンパク質輸送システムによる幹細胞のエピゲノム制御 (2012)
  • Author(s): 安原徳子 他
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] importin α1による塩基性核局在シグナル配列の認識様式 (2012)
  • Author(s): 木本千裕 他
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Estrogen receptor α Subcellular Distribution and Functions. (2012)
  • Author(s): Moriyama T, Oka M, Yoneda Y
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] The novel role of the nucleocytoplasmic transport system in cell differentiation. (2012)
  • Author(s): Yasuhara N 他
  • Organizer: 染色体ワークショップ・核ダイナミクス研究会
  • Place of Presentation: 淡路島
  • Invited
• [Presentation] エストロゲンレセプターの核－細胞質間移動機構とその生理的意義の解明
  • Author(s): 盛山 哲嗣, 岡 正啓, 米田 悦啓
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Importinαによるヒストン結合タンパクRBBP4の核内移行
  • Author(s): 辻井 聡, 盛山 哲嗣, 岡 正啓, 米田 悦啓
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Nup98融合遺伝子による細胞がん化のメカニズム
  • Author(s): 岡 正啓, 米田 悦啓
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜