Microtubule-dependent dorsal determination in vertebrate embryogenesis

• Project/Area Number: 24657152
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Nagoya University
• Principal Investigator: HIBI Masahiko 名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 体軸形成 / ゼブラフィッシュ / 胚発生 / 遺伝学 / 微小管 / 背側決定 / モータータンパク質 / Wntシグナル / 変異体 / 小胞輸送

Research Abstract

In amphibian and teleost embryos, dorsal determinants are initially localized to the vegetal pole and then transported to the dorsal side of the embryo along a microtubule array. The dorsal determinants activate the canonical Wnt pathway that is required for the formation of dorsal tissues. Using transgenic zebrafish lines expressing GFP-Tubulin and EB1-GFP, we found that a parallel microtubule array formed at the vegetal pole. The plus end of microtubule array was directed to the future dorsal side. The microtubules themselves moved to the future dorsal side and the vesicles moved along with vegetal microtubules. We found that Syntabulin, which is required for dorsal determination, surrounded vesicles and moved along with them to the dorsal side. Syntabulin interacted with Grip2a and Wntelss. Our data suggest that Syntabulin interacts with Grip2a and Wntless to recruit the vesicles containing the dorsal determinants onto the microtubules to transport them to the dorsal side.

Research Products

• [Journal Article] Sox5 functions as a fate switch in medaka pigment cell development (2014)
  • Author(s): Nagao Y, Suzuki T, Shimizu A, Kimura T, Seki R, Adachi T, Inoue C, Omae Y, Kamei Y, Hara I, Taniguchi Y, Naruse K, Wakamatsu Y, Kelsh RN, Hibi M, Hashimoto H.
  • Journal Title: PLoS Genetics Volume: 10
  • Peer Reviewed
• [Journal Article] Sox5 functions as a fate switch in medaka pigment cell development. (2014)
  • Author(s): Nagao Y, Suzuki T, Shimizu A, Kimura T, Seki R, Adachi T, Inoue C, Omae Y, Kamei Y, Hara I, Taniguchi Y, Naruse K, Wakamatsu Y, Kelsh RN, Hibi M, Hashimoto H
  • Journal Title: PLoS genetics Volume: 10(4) Issue: 4 Pages: e1004246-e1004246
  • DOI: 10.1371/journal.pgen.1004246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish (2013)
  • Author(s): Kwon HB, Fukuhara S, Asakawa K, Ando K, Kashiwada T, Kawakami K, Hibi M, Kwon YG, Kim KW, Alitalo K, Mochizuki, N.
  • Journal Title: Development Volume: 140 Pages: 4081-4090
  • Peer Reviewed
• [Journal Article] The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish (2013)
  • Author(s): Kwon HB, Fukuhara S, Asakawa K, Ando K, Kashiwada T, Kawakami K Hibi M, Kwon YG, Kim KW, Alitalo K, Mochizuki N.
  • Journal Title: Development Volume: 140 Issue: 19 Pages: 4081-4090
  • DOI: 10.1242/dev.091702
  • Peer Reviewed
• [Journal Article] Requirement of zebrafish ataxin-7 in differentiation of photoreceptors and cerebellar neurons (2012)
  • Author(s): Yanicostas C, Barbieri E, Hibi M, Brice A, Stevanin G, Soussi-Yanicostas N.
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] Dynamic microtubules are the vegetal cortex predict the embryonic axis in zebrafish (2012)
  • Author(s): Tran LD, Hino H, Quach H, Lim S, Shindo A, Mimori-Kiyosue Y, Mione M, Ueno N, Winkler C, Hibi M, Sampath K.
  • Journal Title: Development Volume: 139 Pages: 3644-3652
  • Peer Reviewed
• [Journal Article] Lesion-induced generation of interneuron cell types in specific dorso-ventral domains in the spinal cord of adult zebrafish (2012)
  • Author(s): Kuscha V, Fraser SL, Dias TB, Hibi M, Becker T, Becker CG.
  • Journal Title: J. Comp. Neurol Volume: 530 Pages: 3604-3616
  • Peer Reviewed
• [Journal Article] Requirement of zebrafish ataxin-7 in differentiation of photoreceptors and cerebellar neurons. (2012)
  • Author(s): Yanicostas, C., Barbieri, E., Hibi, M., Brice, A., Stevanin, G., and Soussi-Yanicostas, N.
  • Journal Title: PLoS One Volume: 7 Pages: 250705-250705
  • Peer Reviewed
• [Journal Article] Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish. (2012)
  • Author(s): Tran, L.D.
  • Journal Title: Development Volume: 139 Issue: 19 Pages: 3644-3652
  • DOI: 10.1242/dev.082362
  • Peer Reviewed
• [Journal Article] Lesion-induced generation of interneuron cell types in specific dorso-ventral domains in the spinal cord of adult zebrafish (2012)
  • Author(s): Kuscha, V., Fraser, S. L., Dias, T. B., Hibi, M., Becker, T., and Becker, C. G.
  • Journal Title: J. Comp. Neurol Volume: (in press) Issue: 16 Pages: 3604-3616
  • DOI: 10.1002/cne.23115
  • Peer Reviewed
• [Journal Article] The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution. (2012)
  • Author(s): Yuuta Moriyama
  • Journal Title: Current Biology Volume: 21 Issue: 7 Pages: 601-607
  • DOI: 10.1016/j.cub.2012.01.063
  • Peer Reviewed
• [Presentation] Microtubule-mediated dorsal determination (2014)
  • Author(s): Hibi M, Hino H, Seki R, Shimizu T, Pelegri F.
  • Organizer: 6^<th> Asia Oceania Zebrafish Meeting
  • Place of Presentation: 香港、中国
  • Year and Date: 2014-01-21
  • Invited
• [Presentation] Moving microtubule-mediated dorsal determination (2013)
  • Author(s): Hino H, Seki R, Shimizu T, Pelegri F, Hibi M.
  • Organizer: 第19回小型魚類研究会
  • Place of Presentation: 仙台
  • Year and Date: 2013-09-20
• [Presentation] Microtubule-Dependent Dorsal Determination in Zebrafish (2013)
  • Author(s): Hibi M.
  • Organizer: CDB Symposium 2013
  • Place of Presentation: 理化学研究所神戸
  • Year and Date: 2013-03-05
  • Invited
• [Presentation] Involvement of microtubule-dependent transport of Wnt signaling components in zebrafish dorsal determination (2013)
  • Author(s): Hino H, Seki R, Shimizu T, Long TD, Sampath K, Hibi M.
  • Organizer: 第46回日本発生生物学会大会
  • Place of Presentation: 松江
• [Presentation] Involvement of microtubule-dependent transport of Wnt signaling components in zebrafish dorsal determination. (2013)
  • Author(s): Hino H, Seki R, Shimizu T, Long TD, Sampath K, Hibi M.
  • Organizer: 第46回日本発生生物学会大会
  • Place of Presentation: 松江
• [Presentation] Microtubule-dependent dorsal determination in zebrafish (2012)
  • Author(s): Hino H, Seki R, Long TD, Sampath K, Shimizu T, Hibi M.
  • Organizer: 第45回日本発生生物学会合同大会
  • Place of Presentation: 神戸
• [Presentation] Syntabulin-mediated transport of Wnt signaling machinery in zebrafish dorsal determination (2012)
  • Author(s): Seki R, Hino H, Takeda S, Pelegri F, Hibi M.
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都
• [Presentation] Formation of microtubule array for dorsal determination in zebrafish (2012)
  • Author(s): Hino H, Seki R, Long TD, Sampath K, Hibi M.
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都
• [Presentation] Role of basement membrane in axogenesis of cerebellar granule cells in zebrafish (2012)
  • Author(s): 日比正彦
  • Organizer: Asia-Pacific Developmental Biology Conference
  • Place of Presentation: 台北（台湾）
• [Presentation] Microtubule-mediated dorsal determination. 6th Asia Oceania Zebrafish Meeting.
  • Author(s): Hibi M, Hino H, Seki R, Shimizu T, Pelegri F.
  • Organizer: 6th Asia Oceania Zebrafish Meeting
  • Place of Presentation: 香港、中国
  • Invited
• [Presentation] Moving microtubule-mediated dorsal determination.
  • Author(s): Hino H, Seki R, Shimizu T, Pelegri F, Hibi M.
  • Organizer: 第19回小型魚類研究会
  • Place of Presentation: 仙台
• [Presentation] Microtubule-Dependent Dorsal Determination in zebrafish.
  • Author(s): 日比正彦
  • Organizer: CDB symposium 2013
  • Place of Presentation: 理化学研究所（兵庫県）
  • Invited
• [Presentation] 真骨魚類における小脳神経回路形成
  • Author(s): 日比正彦
  • Organizer: 総合研究大学院大学融合「脳の進化」研究会
  • Place of Presentation: 総合研究大学院大学（神奈川県）
  • Invited
• [Presentation] Formation of cerebellar neural circuits in zebrafish.
  • Author(s): 日比正彦
  • Organizer: Swiss-Japanese Developmental Biology Meeting
  • Place of Presentation: 京都ガーデンパレス（京都府）
• [Presentation] Genetic control for neural circuit formation in teleost cerebellum.
  • Author(s): 日比正彦
  • Organizer: 第45回日本発生生物学会合同大会
  • Place of Presentation: 神戸国際会議場（兵庫県）
  • Invited
• [Remarks] 名古屋大学生物機能開発利用研究センター動物器官機能研究分野
  • URL: http://bbc.agr.nagoya-u.ac.jp/~junkei/