Development of universal method for genome modification using zinc finger nuclease
Project/Area Number |
24657156
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
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Research Institution | Hiroshima University |
Principal Investigator |
YAMAMOTO Takashi 広島大学, 理学(系)研究科(研究院), 教授 (90244102)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | バイオテクノロジー / ゲノム編集 / 発生・分化 / 人工ヌクレアーゼ |
Outline of Final Research Achievements |
In this study, I tried to establish the method for gene modifications such as targeted gene knockout and gene knock-in in various animal species using artificial endonucleases (ZFN and TALEN). Using ZFN protein targeting sea urchin HesC gene generated by E. coli, I observed mutations in early stage of sea urchin embryo, indicating that microinjection of ZFN protein can efficiently introduce mutations in target gene compared to that of ZNF mRNA. Moreover, I established construction system for a highly active TALEN (Platinum TALEN) and demonstrated efficient targeted gene disruption using Platinum TALENs in various animal species such as roundworm (C. elegans), fruit fly (D. melanogaster), sea squirt (C. intestinalis), sea urchin (H. pulcherrimus), frog (Xenopus laevis) and newt (P. waltl)
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration.2014
Author(s)
Hayashi T, Sakamoto K, Sakuma T, Yokotani N, Inoue T, Kawaguchi E, Agata K, Yamamoto T, Takeuchi T
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Journal Title
Development, Growth and Differentiation
Volume: 56
Issue: 1
Pages: 115-121
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases2014
Author(s)
Sakane, Y., Sakuma, T., Kashiwagi, K., Kashiwagi, A., Yamamoto, T. and Suzuki, K. T.
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Journal Title
Dev Growth Differ
Volume: 56(1)
Issue: 1
Pages: 108-114
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Tissue-specific and ubiquitous gene knockouts in Ciona by electroporating TALENs provide new approaches to investigate gene functions2014
Author(s)
Treen, N., Yoshida, K., Sakuma, T., Sasaki, H., Kawai, N., Yamamoto, T., Sasakura, Y.
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Journal Title
Development
Volume: 141
Issue: 2
Pages: 481-487
DOI
Related Report
Peer Reviewed
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[Journal Article] TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS (MVA) syndrome.2014
Author(s)
Ochiai H, Miyamoto T, Kanai A, Hosoba K, Sakuma T, Kudo Y, Asami K, Ogawa A, Watanabe A, Kajii T, Yamamoto T, Matsuura S.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 111(4)
Issue: 4
Pages: 1461-6
DOI
Related Report
Peer Reviewed
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[Journal Article] Efficient TALEN construction and evaluation methods for human cell and animal applications.2013
Author(s)
Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S, Yamamoto T.
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Journal Title
Genes to Cells
Volume: (In press)
Issue: 4
Pages: 315-326
DOI
Related Report
Peer Reviewed
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[Journal Article] Production of Sry knockout mouse using TALEN via oocyte injection.2013
Author(s)
Kato T, Miyata K, Sonobe M, Yamashita S, Tamano M, Miura K, Kanai Y, Miyamoto S, Sakuma T, Yamamoto T, Inui M, Kikusui T, Asahara H, Takada S.
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Journal Title
Scientific reports
Volume: 3
Issue: 1
Pages: 3136-3136
DOI
Related Report
Peer Reviewed
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[Journal Article] Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity.2013
Author(s)
Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
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Journal Title
Sci Rep.
Volume: 3
Issue: 1
Pages: 3379-3379
DOI
Related Report
Peer Reviewed
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[Journal Article] Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases.2012
Author(s)
Watanabe T, Ochiai H, Sakuma T, Horch HW, Hamaguchi N, Nakamura T, Bando T, Ohuchi H, Yamamoto T, Noji S, Mito T.
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Journal Title
Nature Communications
Volume: 3
Issue: 1
Pages: 1017-1017
DOI
Related Report
Peer Reviewed
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