| Project/Area Number |
24658012
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Breeding science
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| Research Institution | National Agriculture and Food Research Organization (2014)
National Institute of Agrobiological Sciences (2012-2013) |
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Principal Investigator |
KISHIMOTO Naoki 独立行政法人農業・食品産業技術総合研究機構, 作物研究所企画管理室, 企画チーム長 (00370651)
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| Co-Investigator(Renkei-kenkyūsha) |
MITSUHARA Ichiro 独立行政法人農業生物資源研究所, 植物科学研究領域, 上級研究員 (80370683)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | バイオテクノロジー / 植物 / 遺伝子 / サイレンシング / 育種学 |
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| Outline of Final Research Achievements |
To screen novel DNA fragments showing ability to circumvent position effects in eukaryote genomes, including transcriptional gene silencing (TGS) of transgenes, I planned to establish a method for screening DNAs suppress TGS (Anti-silencing regions: ASRs) from plant genome and to try screening of random DNA library from plant genome using the method. To develop a systematic method to screen such DNAs, I applied the concept of the screening method in Kishimoto et al. (2013), which use a silenced transgenic tobacco plant in which secondary introduced transgene shows obligatory promoter-homology dependent TGS, to this study. I developed a screening method to assay abilities of DNAs suppressing TGS, based on the phenomena that Arabidopsis FWA genomic transgene shows obligatory TGS in the genome. Using this method, I determined which region to suppress TGS most efficiently in ASR602 found by Kishimoto et al. (2013). However I could not construct random genomic library for the screening.
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