Examination of possible mutation bias due to nutritional conditions

• Project/Area Number: 24658060
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: The University of Tokyo
• Principal Investigator: FUJIWARA Toru 東京大学, 農学生命科学研究科, 教授 (80242163)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 栄養進化 / 変異 / ホウ素過剰 / DNA損傷 / 栄養条件 / 進化 / ＤＮＡ損傷

Outline of Final Research Achievements

High boron treatment induces DNA damage. The present study aimed to examine effect of high boron treatment on introduction of mutation in Arabidopsis genome. Arabidopsis have been successively cultivated for several generations with or without high boron treatment. High boron treated plants exhibited severe necrosis, indicating the treatment was done properly. Genome sequence analysis of the successive generation indicated that the rate of mutation per generation is not significantly different between lines cultivated under high boron and lines cultivated under the control condition. There was a tendency that high boron treatment induce deletion more frequently. The list of the gene to which the mutations were introduced was not biased to the genes related to responses to boron conditions.