Mechanism of resistance to osmotic stress by translation inhibitors
| Project/Area Number |
24658066
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Hirosaki University |
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Principal Investigator |
HYOUTA Himeno 弘前大学, 農学生命科学部, 教授 (80208785)
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| Co-Investigator(Renkei-kenkyūsha) |
MUTO Akira 弘前大学, 農学生命科学部, 研究員 (80034635)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 翻訳阻害剤 / 塩ストレス / 浸透圧ストレス / リボソーム / 翻訳 / シグマE / ppGpp |
|---|
| Research Abstract |
Effects of various kinds of drugs on Escherichia coli cell were examined and we found inhibitors of protein synthesis such as chloramphenicol, kasgamycin or tetracycline increase resistance to osmotic (salt) stress. By using qRT-PCR, we also found that these protein synthesis inhibitors prematurely and transiently activate these membrane-related sigma factor, sigma E.
Resistance to osmotic (salt) stress is also provided by serine hydroxamate, which produces ppGpp. We finally found that there are two pathways for salt resistance, ppGpp-dependent and ppGpp-independent pathways.
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process.2013
Author(s)
Guo, Q., Goto, S., Chen, Y., Feng, B., Xu, Y., Muto, A., Himeno, H., Deng, H., Lei, J., Gao, N.
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Journal Title
Nucleic Acids Res.
Volume: 41
Issue: 4
Pages: 2609-2620
DOI
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Peer Reviewed
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[Presentation] Recognition of mRNA length on the ribosome by tmRNA andSmpB.2013
Author(s)
Kurita, D., Miller, M.R., Muto, A., Buskirk, A.R., Himeno, H.
Organizer
Ribosomes Conference 2013
Place of Presentation
Napa Valley, California
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[Presentation] The first translocation in trans-translation.2012
Author(s)
Takada, K., Kurita, D., Naoe, C., Yokogawa, T., Himeno, H., Takemoto, C.
Organizer
第14回日本RNA学会年会
Place of Presentation
仙台
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