Studies on oligotrophic mutants of an aerobic heterotrophic bacterium
Project/Area Number |
24658068
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Applied microbiology
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Research Institution | Tohoku University |
Principal Investigator |
NAGATA Yuji 東北大学, 生命科学研究科, 准教授 (30237531)
|
Co-Investigator(Kenkyū-buntansha) |
TSUDA Masataka 東北大学, 大学院生命科学研究科, 教授 (90172022)
OHTSUBO Yoshiyuki 東北大学, 大学院生命科学研究科, 助教 (40342761)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 炭酸固定 / 独立栄養細菌 / 環境細菌 / 極貧有機炭素源環境 / 微生物機能開発 |
Research Abstract |
An aerobic and heterotrophic bacterium Sphingobium japonicum UT26 shows oligotrophic growth phenotype, i.e., growth on minimal salt medium without the supply of any carbon source, by the over expression of the adhX gene. Transposon mutagenesis toward the UT26 variant over-expressing the adhX gene and the subsequent analysis of the mutants demonstrated that the putative acetyl-CoA synthetase, pyruvate/phosphate dikinase, and isocitrate lyase genes are necessary for the oligotrophic phenotype. By combining with the genome information of UT26, it was strongly suggested that glyoxylate cycle accompanying with the CO2-fixation process catalyzed by phosphoenolpyruvate carboxylase is involved in the oligotrophic phenotype of the UT26 variant.
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Report
(3 results)
Research Products
(85 results)
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[Journal Article] Complete genome sequence of the thermophilic PCB degrader Geobacillus sp. JF8 (NBRC 109937)2014
Author(s)
Shintani, M., Y. Ohtsubo, K. Fukuda, A. Hosoyama, S. Ohji, A. Yamazoe, N. Fujita, Y. Nagata, M. Tsuda, T. Hatta, and K. Kimbara
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Journal Title
Genome Announcements
Volume: 2
Issue: 1
DOI
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Peer Reviewed
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[Journal Article] ParI, an orphan ParA family protein from Pseudomonas putida KT2440-specific genomic island, interferes with the partition system of IncP-7 plasmids2012
Author(s)
Miyakoshi, M., M. Shintani, K. Inoue, T. Terabayashi, F. Sai, M. Ohkuma, H. Nojiri, Y. Nagata, M. Tsuda
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Journal Title
Environ Microbiol
Volume: 14
Issue: 11
Pages: 2946-2959
DOI
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Peer Reviewed
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[Presentation] 土壌微生物の移植メタゲノム2014
Author(s)
加藤広海, 森宙史, 丸山史人, 豊田敦, 永山浩史, 大坪嘉行, 永田裕二, 藤山秋佐夫, 黒川顕, 津田雅孝
Organizer
第8回日本ゲノム微生物学会年会
Place of Presentation
東京
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[Presentation] Isolation of phenol-catabolic genes by cultivation-independent functional screening from metagenome of soil artificially polluted by aromatic hydrocarbons2013
Author(s)
Hirofumi Nagayama, Tomonori Sugawara, Ryo Endo, Hiromi Kato, Yoshiyuki Ohtsubo, Yuji Nagata, Hiroshi Mori, Ken, Kurokawa, and Masataka Tsuda
Organizer
2nd Thunen Symposium on Soil Metagenomics: mining and learning from metagenomes plus workshop on bioinformatic tools for soil microbiologists
Place of Presentation
Braunschweig, German
Related Report
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[Presentation] Design and experimental validation of a novel non-degenerate universal primer set that amplifies prokaryotic 16S rRNA genes with a low possibility to amplify eukaryotic rRNA genes2013
Author(s)
Hiroshi Mori, Fumito Maruyama, Hiromi Kato, Atsushi Toyoda, Ayumi Dozono, Yoshiyuki Ohtsubo, Yuji Nagata, Asao Fujiyama, Masataka Tsuda, and Ken Kurokawa
Organizer
2nd Thunen Symposium on Soil Metagenomics: mining and learning from metagenomes plus workshop on bioinformatic tools for soil microbiologists
Place of Presentation
Braunschweig, German
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