Mechanism analysis of the extracellular polysaccharide formation form cyanobacteria

• Project/Area Number: 24658090
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Tohoku University
• Principal Investigator: Uozumi Nobuyuki 東北大学, 工学(系)研究科(研究院), 教授 (40223515)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: バイオフィルム / 光合成 / 多糖 / ポリアミン / ラン藻 / 輸送体 / 浸透圧 / 環境ストレス / Synechocystis / 塩ストレス / 糖分泌 / 二成分制御系 / 概日性 / カリウム / オペロン / 二成分系

Outline of Final Research Achievements

Cyanobacteria are prokaryotic microorganisms that perform oxygenic photosynthesis and are adapted to a regular cycle of light and dark periods. We found that high salt stress led to the biofilm formation of the bacteria from planktonic cells. The morphological change reflects to the adaptation to the environmental changes. To better understand the molecular mechanism, we have examined the production of polyamine, which is related with cell growth, and signal transduction pathway. The polyamine synthetic enzymes were found to be involved in the biofilm formation. Some of histidine kinases also participate in the pathway to regulate the formation of biofilm. One of the histidine kinases in K uptake transport system acted as a component in response with salt stress. The expressions were regulated by biological clock, which that help to contribute to the survival of the cells living in a stressful, light-dark cycle environment.

Research Products

• [Int'l Joint Research] Padova University(Italy)
• [Journal Article] Ion channels in plant bioenergetic organelles chloroplast and mitochondria: from molecular identification to function. (2016)
  • Author(s): Carraretto, L., Teardo, E., Checchetto, V., Finazzi, G., Uozumi, N., and Szabo, I.
  • Journal Title: Mol. Plant Volume: 2016 Issue: 3 Pages: 371-395
  • DOI: 10.1016/j.molp.2015.12.004
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] HKT transporters mediate salt stress resistance in plants: from structure and function to the field. (2015)
  • Author(s): Hamamoto, S., Horie, T., Hauser, F., Deinlein, U., Schroeder J.I. and Uozumi, N.
  • Journal Title: Current Opinion in Biotechnology Volume: 32 Pages: 113-120
  • DOI: 10.1016/j.copbio.2014.11.025
  • Peer Reviewed / Open Access
• [Journal Article] Comparative analysis of kdp and ktr mutants reveals distinct roles of the potassium transporters in the model cyanobacterium Synechocystis sp. PCC 6803 (2014)
  • Author(s): Nanatani, K., Shijuku, T., Takano, Y., Zulkifli, L., Yamazaki, Y., Tominaga, A., Souma, S., Onai, K., Morishita, M., Ishiura, M., Hagemann, M., Suzuki, I., Maruyama, H., Arai, F., Uozumi, N.
  • Journal Title: J. Bacteriology Volume: - Issue: 4 Pages: 676-87
  • DOI: 10.1128/jb.02276-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp. PCC 6803 (2013)
  • Author(s): Nanatani K, Shijuku T, Akai M, Yukutake Y, Yasui M, Onai K, Morishita M, Ishiura M, Uozumi N
  • Journal Title: Channels Volume: 7 Issue: 4 Pages: 238-242
  • DOI: 10.4161/chan.25350
  • Peer Reviewed
• [Journal Article] Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp (2011)
  • Author(s): Akai, M., Onai, K., Kusano, M., Sato, M., Redestig, H., Toyooka, K., Morishita, M., Miyake, H., Hazama, A., Checchetto, V., Szabo, I., Matsuoka, K., Saito, K., Yasui, M., Ishiura, M., and Uozumi, N
  • Journal Title: PCC 6803. J. Biol Volume: 286 Issue: 28 Pages: 25224-25235
  • DOI: 10.1074/jbc.m111.236380
  • Peer Reviewed
• [Presentation] シアノバクテリアのポリアミンを介したバイオフィルム誘導機構の 解明 (2015)
  • Author(s): 西坂敦、解良康太、七谷圭、佐伯千香、Nur Illany binti Mohd Siran、牧野恒平、魚住信之
  • Organizer: 藍藻の分子生物学2015
  • Place of Presentation: かずさアカデミアホール 木更津市
  • Year and Date: 2015-11-16
• [Presentation] Synechocystis sp. PCC6803の高塩ストレス誘導型バイオフィルム形成におけるヒスチジンキナーゼhik43の解析 (2015)
  • Author(s): 吉澤優一朗，永山達也，解良康太，七谷圭，鈴木石根，魚住信之
  • Organizer: 藍藻の分子生物学2015
  • Place of Presentation: かずさアカデミアホール 木更津市
  • Year and Date: 2015-11-16
• [Presentation] シアノバクテリアSynechocystis sp. PCC6803の塩ストレス誘導性バイオフィルム形成に必要なヒスチジンキナーゼの同定とシグナル伝達機構 (2015)
  • Author(s): 永山達也、久米井智裕、牧野恒平、井田智章、赤池孝章、鈴木石根、七谷 圭、魚住信之
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2015-03-28 – 2015-03-30
• [Presentation] ラン藻の塩誘導性バイオフィルム形成に関与する二成分系c-di-GMP合成酵素の同定 (2014)
  • Author(s): 牧野恒平，七谷圭，井田智章，澤智裕，佐伯千香，鈴木石根，兵藤守，早川芳宏，赤池孝章，魚住信之
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] シアノバクテリア Synechocystis sp. PCC 6803の塩ストレスによるバイオフィルム形成誘導メカニズムの解析 (2014)
  • Author(s): 牧野恒平, 富永昴, 堀籠智子, 三浦のぞみ, 七谷圭, 相馬聡, 茅森俊介, 佐伯千香, 鈴木石根, 魚住信之
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 川崎市
• [Presentation] Synechocystis sp. PCC6803の細胞外多糖生産を調節する機構解析 (2013)
  • Author(s): 富永昴、三浦のぞみ、佐伯千香、七谷圭、魚住信之
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台市
• [Presentation] Synechocystis sp. PCC 6803 の浸透圧調節に関与するKdp系Kトランスポーターの機能解析 (2012)
  • Author(s): 七谷圭、四十九俊彰、高野洋佑、山崎智子、Lalu Zulkifli、赤井政郎、飯塚龍、松本秀之、丸山央峰、新井史人、魚住信之
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸市
• [Presentation] Membrane transport system conferring the salinity tolerance to bacteria and plant cells. (2012)
  • Author(s): Uozumi, N., Nanatani K., Hamamoto, S.
  • Organizer: 6th Japan-Finland Biotechnology Symposium
  • Place of Presentation: 仙台市