Construction of novel platform for development of functional food products based on next-generation integrated omics approach

• Project/Area Number: 24658129
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: Keio University
• Principal Investigator: FUKUDA Shinji 慶應義塾大学, 政策・メディア研究科, 特任准教授 (80435677)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 腸内細菌 / 統合オミクス / プレバイオティクス / 腸内エコシステム / オリゴ糖 / 機能性食品 / メタボローム / IgA / 腸内環境 / オミクス / プロバイオティクス

Research Abstract

Fructooligosaccharide (FOS), a prebiotic well known for its health-promoting properties, can improve the human gut ecosystem most likely through changes in its microbial composition. However, the detailed mechanism(s) of action of FOS in the modulation of the gut ecosystem remains obscure. Taking advantage of our novel microbe-metabolite correlation approach, combined with fecal IgA measurements, we found that the induction of mucosal IgA by FOS supplementation correlated with the presence of specific gut microbes in human trials. Furthermore, specific metabolic changes were observed positively or negatively correlated with IgA profiles. IgA is well known as an important molecule not only to prevent intestinal pathogenic infection, but also to regulate commensal gut microbiota; therefore, induction of mucosal IgA by FOS supplementation is considered to be one of the beneficial effect of prebiotics through gut ecosystem modification.

Research Products

• [Journal Article] Multiple omics uncovers host-gut microbial mutualism during prebiotic fructooligosaccharide supplementation (2014)
  • Author(s): Kato, T., Fukuda, S., Fujiwara, A., Suda, W., Hattori, M., Kikuchi, J., Ohno, H.
  • Journal Title: DNA Res. Volume: (in press)
  • Peer Reviewed
• [Journal Article] In vitro evaluation method for screening of candidate prebiotic foods (2014)
  • Author(s): Date, Y., Nakanishi, Y., Fukuda, S., Nuijima, Y., Kato, T., Umehara, M., Ohno, H., and Kikuchi, J.
  • Journal Title: Food Chem. Volume: 152 Pages: 251-260
  • Peer Reviewed
• [Journal Article] Gut microbiome and metabolic diseases (2014)
  • Author(s): Fukuda, S. and Ohno, H.
  • Journal Title: Semin. Immunopathol Volume: 36 Pages: 103-114
  • Peer Reviewed
• [Journal Article] Multiple omics uncovers host-gut microbial mutualism during prebiotic fructooligosaccharide supplementation (2014)
  • Author(s): Kato, T., Fukuda, S. et al.
  • Journal Title: DNA Research Volume: 21
  • Peer Reviewed
• [Journal Article] In vitro evaluation method for screening of candidate prebiotic foods (2014)
  • Author(s): Date, Y., Nakanishi, Y., Fukuda, S. et al.
  • Journal Title: Food Chemistry Volume: 152 Pages: 251-260
  • DOI: 10.1016/j.foodchem.2013.11.126
  • Peer Reviewed
• [Journal Article] Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells (2013)
  • Author(s): Furusawa, Y., Obata, Y., Fukuda, S., Endo, T. A., Nakato, G., Takahashi, D., Nakanishi, Y., Uetake, C., Kato, K., Kato, T., Takahashi, M., Fukuda, N.N., Murakami, S., Miyauchi, E., Hino, S., Atarashi, K., Onawa, S., Fujimura, Y., Lockett, T., Clarke, J.M., Topping, D.L., Tomita, M., Hori, S., Ohara, O., Morita, T., Koseki, H., Kikuchi, J., Honda, K., Hase, K., Ohno, H.
  • Journal Title: Nature Volume: 504 Pages: 446-450
  • Peer Reviewed
• [Journal Article] Robustness of gut microbiota of healthy adults in response to probiotic intervention revealed by high-throughput pyrosequencing (2013)
  • Author(s): Kim, S.W., Suda, W., Kim, S., Oshima, K., Fukuda, S., Ohno, H., Morita, H., Hattori, M.
  • Journal Title: DNA Res. Volume: 20 Pages: 241-253
  • Peer Reviewed
• [Journal Article] Microbiota-derived lactate accelerates colon epithelial cell turnover in starvation-refed mice (2013)
  • Author(s): Okada, T., Fukuda, S., Hase, K., Nishiumi, S., Izumi, Y., Yoshida, M., Hagiwara, T., Kawashima, R., Yamazaki, M., Oshio, T., Otsubo, T., Inagaki, O. K., Kakimoto, K., Higuchi, K., Kawamura, Y. I., Ohno, H., Dohi, T.
  • Journal Title: Nat. Commun. Volume: 4 Pages: 1654-1654
  • NAID: 40020315108
  • Peer Reviewed
• [Journal Article] 統合オミクスによる腸エコシステムの全容理解に向けた取り組み (2013)
  • Author(s): 福田真嗣、大野博司
  • Journal Title: 細胞工学 Volume: 32 Pages: 1138-1142
• [Journal Article] 腸内環境とアンチエイジング (2013)
  • Author(s): 福田真嗣、村上慎之介
  • Journal Title: 日本抗加齢医学会雑誌 Volume: 9 Pages: 717-722
• [Journal Article] Robustness of gut microbiota of healthy adults in response to probiotic intervention revealed by high-throughput pyrosequencing. (2013)
  • Author(s): Kim, SW.
  • Journal Title: DNA Research Volume: 20(in press) Issue: 3 Pages: 241-253
  • DOI: 10.1093/dnares/dst006
  • Peer Reviewed
• [Journal Article] Microbiota-derived lactate accelerates colon epithelial cell turnover in starvation-refed mice (2013)
  • Author(s): Okada T
  • Journal Title: Nat. Commun Volume: 4巻 Issue: 1 Pages: 1654-1654
  • DOI: 10.1038/ncomms2668
  • Peer Reviewed
• [Journal Article] Thermophile-fermented compost as a possible scavenging feed additive to prevent peroxidation. (2013)
  • Author(s): Miyamoto, H.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 115(in press) Issue: 2 Pages: 203-208
  • DOI: 10.1016/j.jbiosc.2013.01.024
  • Peer Reviewed
• [Journal Article] Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters (2012)
  • Author(s): Fukuda, S., Toh, H., Taylor, T. D., Ohno, H., and Hattori, M.
  • Journal Title: Gut Microbes Volume: 3 Pages: 449-454
  • Peer Reviewed
• [Journal Article] 統合オミクスによる腸エコシステムの理解 (2012)
  • Author(s): 福田真嗣、大野博司
  • Journal Title: 実験医学 Volume: 30 Pages: 3255-3260
• [Journal Article] Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations. (2012)
  • Author(s): Kobayashi A, Donaldson DS, Kanaya T, Fukuda S, Baillie JK, Freeman TC, Ohno H, Williams IR, Mabbott NA.
  • Journal Title: DNA Res. Volume: 19(5) Issue: 5 Pages: 407-22
  • DOI: 10.1093/dnares/dss022
  • Peer Reviewed
• [Journal Article] Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters. (2012)
  • Author(s): Fukuda, S.
  • Journal Title: Gut Microbes Volume: 3 Issue: 5 Pages: 449-454
  • DOI: 10.4161/gmic.21214
  • Peer Reviewed
• [Journal Article] The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells (2012)
  • Author(s): Kanaya, T., Hase, K., Takahashi, D., Fukuda, S., Hoshino, K., Sasaki, I., Hemmi, H., Knoop, K. A., Kumar, N., Sato, M., Katsuno, T., Yokosuka, O., Toyooka, K., Nakai, K., Sakamoto, A., Kitahara, Y., Jinnohara, T., McSorley, S. J., Kaisho, T., Williams, I. R., and Ohno, H.
  • Journal Title: Nat Immunol Volume: 13 Issue: 8 Pages: 729-736
  • DOI: 10.1038/ni.2352
  • Peer Reviewed
• [Journal Article] Exposure to probiotic Lactobacillus acidophilus L-92 modulates gene expression profiles of epithelial Caco-2 cells. (2012)
  • Author(s): Yanagihara, S.
  • Journal Title: Journal of Medicinal Food Volume: 15 Issue: 6 Pages: 511-519
  • DOI: 10.1089/jmf.2012.0040
  • Peer Reviewed
• [Presentation] 統合オミクスによる宿主-腸内細菌間相互作用の理解 (2014)
  • Author(s): 福田真嗣
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-30
  • Invited
• [Presentation] Understanding the complex gut ecosystem via integrated omics approach (2014)
  • Author(s): 福田真嗣
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-26
  • Invited
• [Presentation] 腸内エコシステムの理解に慶應義塾大学・政策・メディア研究科・特任よる新たな健康増進ストラテジー (2014)
  • Author(s): 福田真嗣
  • Organizer: 第3回アンチエイジングセミナー
  • Place of Presentation: 東京
  • Year and Date: 2014-01-23
  • Invited
• [Presentation] 統合オミクスによる腸内エコシステムの理解 (2013)
  • Author(s): 福田真嗣
  • Organizer: 第29回日本微生物生態学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2013-11-24
  • Invited
• [Presentation] Human Microbiomeと生体応答の理解による新たな健康増進ストラテジー (2013)
  • Author(s): 福田真嗣
  • Organizer: 第13回抗加齢医学の実際
  • Place of Presentation: 東京
  • Year and Date: 2013-09-16
  • Invited
• [Presentation] Comprehensive understanding of gut ecosystem via multiple omics approach (2013)
  • Author(s): Fukuda, S
  • Organizer: International Conference of Matryoshka-type Evolution of Eukaryotic Cells
  • Place of Presentation: Kyoto
  • Year and Date: 2013-07-25
  • Invited
• [Presentation] マルチオミクスによる腸内共生システムの理解 (2012)
  • Author(s): 福田真嗣
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
  • Invited
• [Presentation] Multiple omics approach identified acetate produced by probiotic bifidobacteria to protect host from enteropathogenic infection (2012)
  • Author(s): Fukuda, S
  • Organizer: International Conference and Exhibition of Probiotics
  • Place of Presentation: San Antonio, TX
  • Year and Date: 2012-11-19
  • Invited
• [Presentation] Understanding the gut ecosystem via multiple omics approach (2012)
  • Author(s): Fukuda, S
  • Organizer: The International Scientific Conference on Probiotics and Prebiotics 2012
  • Place of Presentation: Kosice, Slovakia
  • Year and Date: 2012-06-12
  • Invited
• [Presentation] Multiple omics approach identified acetate produced by probiotic bifidobacteria to protect host from enteropathogenic infection. (2012)
  • Author(s): Fukuda, S.
  • Organizer: International Conference and Exhibition of Probiotics
  • Place of Presentation: San Antonio, USA
  • Invited
• [Presentation] Understanding the gut ecosystem via multiple omics approach. (2012)
  • Author(s): Fukuda, S.
  • Organizer: The International Scientific Conference on Probiotics and Prebiotics 2012
  • Place of Presentation: Kosice, Slovakia
  • Invited
• [Presentation] 腸内エコシステムの理解に向けたマルチオーミクス解析技術の構築 (2012)
  • Author(s): 福田真嗣
  • Organizer: 第16回腸内細菌学会
  • Place of Presentation: 神戸
  • Invited
• [Presentation] 腸内エコシステムの理解による新たな健康増進ストラテジー
  • Author(s): 福田真嗣
  • Organizer: 第3回アンチエイジングセミナー
  • Place of Presentation: 東京
  • Invited
• [Book] 微生物機能学 (2012)
  • Author(s): 福田真嗣
  • Publisher: 三共出版
• [Remarks]
  • URL: http://www.iab.keio.ac.jp/jp/