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A basic study for measurements of in situ soil enzymatic activities

Research Project

Project/Area Number 24658200
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Irrigation, drainage and rural engineering/Rural planning
Research InstitutionThe University of Tokyo

Principal Investigator

DOI Ryoichi  東京大学, 農学生命科学研究科, 助教 (20587125)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsリグノセルロース分解活性 / 土壌微生物 / 原位置土壌酵素 / 土壌水分 / 開発適正技術 / 土壌酵素 / 現位置測定 / フォルマザンの毒性 / リグノセルロース分解 / 現位置土壌酵素 / デヒドロゲナーゼ / セルラーゼ / 強度 / タイ / チュニジア / 土壌カラム / デヒドロゲナーゼ活性
Outline of Final Research Achievements

A method for in situ soil enzyme activity measurement with neither the destruction of soil structure nor the alternation of soil environmental components such as pH was examined. Changes in bending strength of bamboo barbecue skewer were determined by inserting the bamboo skewer into a field soil collected in Tochigi prefecture. The soil-skewer system underwent one of the following three treatments, namely, water saturation, water-submerging, and two saturation-to-dry cycles within two weeks. The saturation-to-dry treatment resulted in the most significant reduction of bending strength, hence the applicability of the current method to activity measurements of in situ soil enzymes was indicated.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] Precise micromolar-level glucose determination using a glucose test strip for quick and approximate millimolar-level estimation2014

    • Author(s)
      Ryoichi DOI
    • Journal Title

      Anal. Methods

      Volume: 6 Issue: 23 Pages: 9509-9513

    • DOI

      10.1039/c4ay02219j

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Wet/dry Paddy Cycles to Optimize Rice Production for Total Quality of Life: Regarding Rice as a Water-resistant Plant2013

    • Author(s)
      Ryoichi DOI and Supachai PITIWUT
    • Organizer
      The 10th INWEPF Steering Meeting and Symposium
    • Place of Presentation
      Chiang Mai, Thailand
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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