| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Germ cell-specific genes such as Ddx4, Dnd1, and Dazl play critical roles in the proliferation and survival of germ cells. The methylation levels of these genes and the pluripotency marker gene in chicken sperm were investigated in relation to gene expression. The putative promoters of these genes showed very low methylation levels in sperm, but they were highly methylated in the liver. Conversely, the Alb gene promoter was highly methylated in sperm. The results that low methylation was in germ cell-specific genes but high methylation in non-specific genes may indicate selective DNA methylation of specific genes in chicken sperm. In addition, these germ cell-specific genes and the pluripotency marker gene may be ready to express any time after fertilization.
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