Epigenetic analysis of the germ cell-specific genes in chicken germ-line specific cells
Project/Area Number |
24658246
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Basic veterinary science/Basic zootechnical science
|
Research Institution | Kyushu University |
Principal Investigator |
HATTORI Masa-aki 九州大学, (連合)農学研究科(研究院), 教授 (60175536)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | エピジェネティック / ニワトリ生殖細胞 / 精子 / 生殖細胞マーカー遺伝子 / 未分化細胞マーカー遺伝子 / メチル化遺伝子 / 生殖系列幹細胞 / 生殖細胞特異的遺伝子 / 転写開始点上流域のメチル化 / Nanog遺伝子 / アルブミン遺伝子 / メチル化酵素発現 / 国際情報交換 / フランス |
Outline of Final Research Achievements |
Germ cell-specific genes such as Ddx4, Dnd1, and Dazl play critical roles in the proliferation and survival of germ cells. The methylation levels of these genes and the pluripotency marker gene in chicken sperm were investigated in relation to gene expression. The putative promoters of these genes showed very low methylation levels in sperm, but they were highly methylated in the liver. Conversely, the Alb gene promoter was highly methylated in sperm. The results that low methylation was in germ cell-specific genes but high methylation in non-specific genes may indicate selective DNA methylation of specific genes in chicken sperm. In addition, these germ cell-specific genes and the pluripotency marker gene may be ready to express any time after fertilization.
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Report
(4 results)
Research Products
(5 results)