| Project/Area Number |
24658256
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Gifu University |
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Principal Investigator |
FUKUSHI Hideto 岐阜大学, 応用生物科学部, 教授 (10156763)
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| Co-Investigator(Kenkyū-buntansha) |
OHYA Kenji 岐阜大学, 応用生物科学部, 准教授 (50402219)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | アンチセンス鎖 / ヘルペスウイルス / 遺伝子発現制御 / アンチセンスRNA |
|---|
| Research Abstract |
Genetic information is transcribed from DNA to RNA as mRNA. And the RNA called anti-sense RNA is transcribed from the antisense strand of DNA. The purpose of this study was to clarify the function of the anti-sense RNA found in equine herpesvirus infected cells. The miRNA was investigated and found that expression of viral miRNA was almost nothing. On the other hand, miRNA derived from the host genome showed variation of expression level according to the infection of virus. Transcription initiation sites were identified including the anti-sense RNAs. Double stranded RNA was extracted from the infected cells but their nucleotide sequences were not determined in the present study.
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