Development of YAP-dependent hepatic tumor formation and analysis of global expression of related microRNAs
| Project/Area Number |
24659030
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NISHINA Hiroshi 東京医科歯科大学, 難治疾患研究所, 教授 (60212122)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | YAP / 肝癌 / 細胞競合 / アポトーシス / cDNA / マイクロRNA / HTVi法 / 網羅的遺伝子解析 / 肝がん |
|---|
| Research Abstract |
Recently, YAP was shown to play an important role in organ size control and to be inhibited by the Hippo signaling pathway. Either transgenic overexpression of YAP or knockout of Hippo pathway genes in mouse liver results in enlargement of this organ and the eventual development of hepatic tumors. In this study, we used the method of hydrodynamic tail vein injection (HTVi) that is specific for gene transfer into liver. As a results, we succeeded in establishing an efficient system of liver cancer induction due to gain of function of the active YAP. Secondly, we analyzed the gene expression by cDNA microarray and identified more than 20 genes whose expression is increased by active YAP. Thus, our results could contribute to elucidate the mechanismof human liver cancer.
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Report
(2 results)
Research Products
(52 results)
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[Journal Article] Cancer Susceptibility andembryonic lethality in Mob1A/1Bdouble mutant mice2012
Author(s)
Miki Nishio, Koichi Hamada, Kohichi Kawahara, Masato Sasaki, Fumihito Noguchi, Shuhei Chiba, Kensaku Mizuno, Satoshi O. Suzuki, Youyi Dong, Masaaki Tokuda, Takumi Morikawa, Hiroki Hikasa, Jonathan Eggenschwiler, Norikazu Yabuta, Hiroshi Nojima, Kentaro Nakagawa, Yutaka Hata, Hiroshi Nishina, Koshi Mimori, Masaki Mori, Takehiko Sasaki, Tak W. Mak, Toru Nakano, Satoshi Itami, and Akira Suzuki
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Journal Title
J. Clin. Invest
Volume: 122(12)
Pages: 4505-4518
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