Differentiation of plasmacytoid dendritic cells from iPS cells and its application for Vaccine development

• Project/Area Number: 24659040
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: KAWABATA Kenji 独立行政法人医薬基盤研究所, 幹細胞制御プロジェクト, プロジェクトリーダー (50356234)
• Co-Investigator(Renkei-kenkyūsha): YAMAGUCHI Tomoko (50580130)
• Project Period (FY): 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 免疫学 / iPS細胞 / ワクチン

Research Abstract

The purpose of this study is to develop the differentiation protocols for plasmacytoid dendritic cells from induced pluripotent stem (iPS) cells. Cytokines, such as GM-CSF and Flt3L, could efficiently promote the differentiation into plasmacytoid dendritic cells from iPS cell-derived hematopoietic progenitor cells. Also, human hematopoietic progenitor cells were induced from human iPS cells by co-culture with OP9 feeder cells. These cells could express CD34 and CD43 as hematopoietic markers, suggesting that our protocols could apply to the differentiation into human plasmacytoid dendritic cells.

Research Products

• [Journal Article] Two-step differentiation of mast cells from induced pluripotent stem cells (2013)
  • Author(s): Yamaguchi T, Tashiro K, Tanaka S, Katayama S, Ishida W, Fukuda K, Fukushima A, Araki R, Abe M, Mizuguchi H, Kawabata K
  • Journal Title: Stem Cells Dev Volume: 22 Pages: 726-734
• [Journal Article] Two-step differentiation of mast cells from induced pluripotent stem cells. (2013)
  • Author(s): Yamaguchi, T., Yamaguchi, T., Tashiro, K., Tanaka, S., Katayama, S., Ishida, W., Fukuda, K., Fukushima, A., Araki, R., Abe, M., Mizuguchi, H., Kawabata, K.
  • Journal Title: Stem Cells Dev. Volume: 22 Issue: 5 Pages: 726-734
  • DOI: 10.1089/scd.2012.0339
  • Peer Reviewed
• [Journal Article] Inhibition of Lnk in mouse induced pluripotent stem cells promotes hematopoietic cell generation (2012)
  • Author(s): Tashiro K, Omori M, Kawabata K, Hirata N, Yamaguchi T, Sakurai F, Takaki S, Mizuguchi H
  • Journal Title: Stem Cells Dev Volume: 21 Pages: 3381-3390
• [Presentation] マウスiPS細胞から成熟したマスト細胞の分化誘導法の開発 (2013)
  • Author(s): 山口朋子
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://www.nibio.go.jp/Proj3HP/index.html
• [Remarks] 研究内容
  • URL: http://www.nibio.go.jp/Proj3HP/studies.html