| Project/Area Number |
24659046
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
FUJII Nobutaka 京都大学, 薬学研究科(研究院), 教授 (60109014)
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| Co-Investigator(Renkei-kenkyūsha) |
OHNO Hiroaki 京都大学, 大学院薬学研究科, 教授 (30322192)
OISHI Shinya 京都大学, 大学院薬学研究科, 講師 (80381739)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 天然物 / 化合物ライブラリー / スクリーニング / アッセイ / 生物活性化合物 / 鏡像体 / 天然有機化合物 |
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| Outline of Final Research Achievements |
Development of a novel screening approach was achieved toward effective uses of chiral compound libraries for drug discovery. Two cancer-related proteins (MDM2, MDMX) with the native structures and functions were prepared via a standard solid-phase peptide synthesis and appropriate folding conditions. Using these synthetic proteins for screening of in-house chemical library and natural product library, a number of hit compounds were identified via a primary chemical array-mediated screening and the subsequent fluorescence polarization assay.
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