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Development of anti-inflammatory drugs based on genomic DNA of lactic acid bacteria

Research Project

Project/Area Number 24659060
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Environmental pharmacy
Research InstitutionFukuoka University

Principal Investigator

KASHIGE Nobuhiro  福岡大学, 薬学部, 教授 (80185751)

Co-Investigator(Kenkyū-buntansha) SATHO Tomomitsu  福岡大学, 薬学部, 助教 (90369025)
IRIE Keiichi  福岡大学, 加齢脳科学研究所, 研究員 (50509669)
Research Collaborator HIRAMATSU Yukihiro  福岡大学, 大学院・薬学研究科, 大学院生
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords乳酸菌 / ゲノムDNA / 抗炎症作用 / toll-like receptor 9 / NF-κB / オリゴデオキシヌクレオチド / TLR9 / NF-kB
Research Abstract

The inhibition of chronic inflammation by long-term use of NSAIDs, an anti-inflammatory drug, leads to the prevention and treatment of cancer. However, NSAIDs induce gastrointestinal side effects. We therefore investigated genomic DNA, which is one of the anti-inflammatory components of lactic acid bacteria (LAB). The use of component allows the application of LAB for systemic tissues except for intestine. The present study showed that genomic DNA of LAB is taken up into Caco-2 cells, and toll-like receptor 9, a specific receptor of bacterial DNA, is one of the major pathways for the anti-inflammatory effects by genomic DNA of LAB. Since the mechanisms of anti-inflammatory effects differ between genomic DNA of LAB and NSAIDs, long-term use of genomic DNA of LAB may lead to the prevention and treatment of cancer without side effects.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (11 results) Remarks (3 results)

  • [Journal Article] Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus casei inhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cells2014

    • Author(s)
      Yukihiro Hiramatsu, Masatatsu Yamamoto, Tomomitsu Satho, Keiichi Irie, Akiko Kai, Saori Uyeda, Yuki Fukumitsu, Akihisa Toda, Takeshi Miyata, Fumio Miake, Takeshi Arakawa, Nobuhiro Kashige
    • Journal Title

      BMC Biotechnology

      Volume: 印刷中

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression and secretion of cholera toxin B subunit in lactobacilli2013

    • Author(s)
      Takahiro Okuno, Nobuhiro Kashige, Tomomitsu Satho, Keiichi Irie, Yukihiro Hiramatsu, Tanjina Sharmin, Yuki Fukmits, Saori Uyeda, Seitaro Yamada, Tetsuya Harakuni, Takeshi Miyata, Takeshi Arakawa, Masumi Imoto, Akihisa Toda, Yukihiko Nakashima, Fumio Miake
    • Journal Title

      Biological Pharmaceutical Bulletin

      Volume: 36(6) Pages: 952-958

    • NAID

      130003361440

    • URL

      https://www.jstage.jst.go.jp/article/bpb/36/6/36_b12-01021/_article

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differences in TLR9-dependent inhibitory effects of H_2O_2-induced IL-8 secretion and NF-kappa B/I kappa B-alpha system activation by genomic DNA from five Lactobacillus species2013

    • Author(s)
      Yukihiro Hiramatsu, Tomomitsu Satho, Keiichi Irie, Shota Shiimura, Takahiro Okuno, Tanjina Sharmin, Yuki Fukumitsu, Saori Uyeda, Yukihiko Nakashima, Fumio Miake, Nobuhiro Kashige
    • Journal Title

      Microbes and Infection

      Volume: 15(2) Issue: 2 Pages: 96-104

    • DOI

      10.1016/j.micinf.2012.11.003

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression and secretion of cholera toxin B subunit in lactobacilli2013

    • Author(s)
      Takahiro Okuno, Nobuhiro Kashige, Tomomitsu Satho, Keiichi Irie, Yukihiro Hiramatsu, Tanjina Sharmin, Yuki Fukmits, Saori Uyeda, Seitaro Yamada, Tetsuya Harakuni, Takeshi Miyata, Takeshi Arakawa, Masumi Imoto, Akihisa Toda, Yukihiko Nakashima, Fumio Miake
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 36(6) Pages: 952-958

    • NAID

      130003361440

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression and secretion of cholera toxin B subunit in lactobacilli2013

    • Author(s)
      Takahiro Okuno, Nobuhiro Kashige, Tomomitsu Satho, et al.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 印刷中

    • NAID

      130003361440

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus casei inhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cells

    • Author(s)
      Yukihiro Hiramatsu, Masatatsu Yamamoto, Tomomitsu Satho, Keiichi Irie, Akiko Kai, Saori Uyeda, Yuki Fukumitsu, Akihisa Toda, Takeshi Miyata, Fumio Miake, Takeshi Arakawa, Nobuhiro Kashige
    • Journal Title

      BMC Biotechnology

      Volume: (In press)

    • Related Report
      2013 Final Research Report
  • [Presentation] 乳酸菌由来DNAの腸管における抗炎症作用2013

    • Author(s)
      平松征洋、佐藤朝光、入江圭一、百武美香、見明史雄、鹿志毛信広
    • Organizer
      第30回日本薬学会九州支部大会
    • Place of Presentation
      長崎国際大学
    • Year and Date
      2013-12-07
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] マウスにおけるDSS誘導性大腸炎の病態とTREM-1/TREM-2の発現量比の一致2013

    • Author(s)
      江川さおり、百武美香、中村智美、平松征洋、上田紗織、入江圭一、佐藤朝光、中島幸彦、三島健一、鹿志毛信広、見明史雄
    • Organizer
      第86回日本生化学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-09-11
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Oligodeoxynucleotide 5'- TTTTGCCG- 3' inhibits intestinal inflammation2013

    • Author(s)
      Yukihiro Hiramatsu, Tomomitsu Satho, Keiichi Irie, Shota Shiimura, Yukihiko Nakashima, Fumio Miake, Nick Carpino, Nobuhiro Kashige
    • Organizer
      World Biotechnology Congress (WBC) 2013
    • Place of Presentation
      Boston, USA
    • Year and Date
      2013-06-04
    • Related Report
      2013 Final Research Report
  • [Presentation] デキストラン硫酸ナトリウム(DSS)投与により発症するマウス大腸炎の病態に関する性差2013

    • Author(s)
      百武美香、田村幸恵、江川さおり、入江圭一、佐藤朝光、平松征洋、上田紗織、福光由起、中島幸彦、三島健一、藤原道弘、鹿志毛信広、見明史雄
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Lactobacillus caseiのゲノムDNAに含まれる抗炎症作用を持つオリゴデオキシヌクレオチドの特定と作用機構の解明2013

    • Author(s)
      平松征洋、佐藤朝光、入江圭一、椎村翔太、中島幸彦、見明史雄、鹿志毛信広
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Oligodeoxynucleotide 5'-TTTTGCCG-3' inhibits intestinal inflammation2013

    • Author(s)
      Yukihiro Hiramatsu, Tomomitsu Satho, Keiichi Irie, Shota Shiimura, Yukihiko Nakashima, Fumio Miake, Nick Carpino, Nobuhiro Kashige
    • Organizer
      World Biotechnology Congress (WBC) 2013
    • Place of Presentation
      Boston, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 乳酸菌ゲノムDNA由来の抗炎症作用を持つオリゴデオキシヌクレオチドの特定2012

    • Author(s)
      椎村翔太、平松征洋、佐藤朝光、入江圭一、宇高彩奈、上田紗織、中島幸彦、鹿志毛信広、見明史雄
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本大学
    • Year and Date
      2012-12-08
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] ヒト結腸癌由来Caco-2細胞における炎症誘発時のProgranulin発現と分泌2012

    • Author(s)
      宇高彩奈、平松征洋、佐藤朝光、椎村翔太、入江圭一、古川歩、三島健一、藤原道弘、中島幸彦、鹿志毛信広、見明史雄
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本大学
    • Year and Date
      2012-12-08
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 5種類のLactobacillus属乳酸菌由来ゲノムDNAの抗炎症作用機構に関する研究2012

    • Author(s)
      平松征洋、佐藤朝光、入江圭一、椎村翔太、中島幸彦、見明史雄、鹿志毛信広
    • Organizer
      第16回腸内細菌学会
    • Place of Presentation
      神戸市産業振興センター
    • Year and Date
      2012-06-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 5種類のLactobacillus属乳酸菌由来ゲノムDNAの抗炎症作用機構に関する研究

    • Author(s)
      平松征洋、佐藤朝光、入江圭一、椎村翔太、中島幸彦、見明史雄、鹿志毛信広
    • Organizer
      第16回腸内細菌学会
    • Place of Presentation
      神戸市産業振興センター「ハーバーホール」
    • Related Report
      2012 Research-status Report
  • [Presentation] デキストラン硫酸ナトリウム(DSS)投与により発症するマウス大腸炎の病態に関する性差

    • Author(s)
      百武美香、田村幸恵、江川さおり、入江圭一、佐藤朝光、平松征洋、上田紗織、福光由起、中島幸彦、三島健一、藤原道弘、鹿志毛信広、見明史雄
    • Organizer
      日本薬学会 第133年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.pha.fukuoka-u.ac.jp/user/microbiology/web/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 福岡大学微生物薬品化学研究室

    • URL

      http://www.pha.fukuoka-u.ac.jp/user/microbiology/web/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 福岡大学微生物薬品化学研究室

    • URL

      http://www.pha.fukuoka-u.ac.jp/user/microbiology/web/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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