Development of new cancer therapy using the exosomes from patients.

Research Project

Project/Area Number	24659166
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Human pathology
Research Institution	Tokyo Medical University
Principal Investigator	NAKAYA Takeo 東京医科大学, 医学部, 助教 (80512277)
Co-Investigator(Kenkyū-buntansha)	KURODA Masahiko 東京医科大学, 医学部, 教授 (80251304)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	がん / エキソソーム / miRNA / CD8 T細胞 / 腫瘍抑制 / microRNA / T細胞 / がん治療開発
Research Abstract	We developed the effective culture system of human CD8 T cells and effective collection methods of CD8 T cell generating exosomes. We also invented the accurate measurement system of T cell exosomes using the surface molecules of CD8 T cells. We also developed the cancer cell targeting exosomes. We investigated the tumor suppressive effects of cancer cell targeting exosomes in mice with human lung cancer cells or sarcoma cells. We observed how the miRNA including exosomes suppress the luciferase expression in luciferase expressing mice. We also investigated the methods that only hepatic cells can intake exosomes using HBV derived surface molecules.