Medium-to-small vessel vasculitis induced by NKT cells that react with vascular endothelial cells
| Project/Area Number | 24659171 |
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Hokkaido University |
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Principal Investigator |
ISHIZU Akihiro 北海道大学, 保健科学研究院, 教授 (60321957)
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| Project Period (FY) |
2012-04-01 – 2014-03-31(Estimated)
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| Project Status |
Completed(Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost : ¥3,000,000、Indirect Cost : ¥900,000)
Fiscal Year 2013 : ¥1,560,000 (Direct Cost : ¥1,200,000、Indirect Cost : ¥360,000)
Fiscal Year 2012 : ¥2,340,000 (Direct Cost : ¥1,800,000、Indirect Cost : ¥540,000)
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| Keywords | 血管炎 / 血管内皮細胞 / 自己免疫 / NKT細胞 / 自己反応性 |
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| Research Abstract |
A T cell clone reactive with rat vascular endothelial cells (REC) was established from the rat model of medium-to-small vessel vasculitis and named VASC-1. Intravenous injection of VASC-1 induced small vessel vasculitis (SVV) in normal recipients. After interaction with REC, several cytokines were produced from VASC-1, which was inhibited by blocking of CD1d on the REC surface. The collective findings indicated VASC-1 as an NKT cell clone. NKT cells are classified into type I and II. Type I NKT cells are characterized by expression of semi-invariant TCRs and potential to bind to marine sponge-derived alpha-galactosylceramide (a-GalCer) loaded on CD1d; whereas, type II NKT cells do not manifest the characteristics. VASC-1 exhibited a usage of TCR other than the type I invariant TCR and did not bind to a-GalCer-loaded CD1d; therefore, it was regarded as a type II NKT cell clone. These evidences suggested that REC-reactive type II NKT cells could be involved in the pathogenesis of SVV.
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Report
(3results)
Research Products
(61results)
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[Journal Article] Abnormal conformation and impaired degradation of NETs induced by propylthiouracil: Implication of disordered NETs in MPO-ANCA-associated vasculitis2012
Author(s)
Nakazawa, D., Tomaru, U., Suzuki, A., Hasegawa, R., Kobayashi, T., Nishio, S., Kasahara, M. and Ishizu, A
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Journal Title
Arthritis Rheum
Volume : 64(11)
Pages : 3779-3787
DOI
Related Report
Peer Reviewed
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[Journal Article] Decreased proteasomal activity causes age-related phenotypes and promotes the development of metabolic abnormalities2012
Author(s)
Tomaru U, Takahashi S, Ishizu A, Miyatake Y, Gohda A, Suzuki S, Ono A, Ohara J, Baba T, Murata S, Tanaka K, Kasahara M
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Journal Title
Am.J.Pathol.
Volume : 180
Pages : 963-972
DOI
Related Report
Peer Reviewed
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[Journal Article] Decrease of peripheral and intestinal NKG2A-positive T cells in patients with ulcerative colitis2012
Author(s)
Katsurada, T., Kobayashi, W., Tomaru, U.,Baba, T., Furukawa, S., Ishizu, A., Takeda, K., Sakamoto, N., Asaka, M., Takeda, H. and Kasahara, M
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Journal Title
DOI
Related Report
Peer Reviewed
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[Presentation] Prediction of prognosis by gene expression profiling of peripheral blood in Japanese patients with MPO-ANCA-associated vasculitis after standard treatment : JMAAV subanalysis2012
Author(s)
Ishizu A, Tomaru U, Murai T, Yamamoto T, Yoshiki T, Yumura W, Yamagata K, Yamada H, Kumagai S, Kurokawa MS, Suka M, Makino H, Ozaki S.
Organizer
The Asia Pacific Meeting of Vasculitis and ANCA Workshop 2012
Place of Presentation
Shinagawa, Tokyo, Japan
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