Structure-function of bacterial mitoNEET homologs

• Project/Area Number: 24659202
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Nippon Medical School
• Principal Investigator: IWASAKI TOSHIO 日本医科大学, 医学部, 講師 (40277497)
• Co-Investigator(Kenkyū-buntansha): KUSANO Teruo 日本医科大学, 医学部, 助教 (30434129) ; KUMASAKA Takashi 高輝度光科学研究センター, 利用研究促進部門, 副主席研究員 (30291066) ; IWASAKI Hideo 早稲田大学, 理工学術院, 教授 (00324393)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: mitoNEET / 鉄硫黄クラスター / 金属蛋白質 / 構造機能生理 / 好熱菌 / シアノバクテリア / レドックス

Research Abstract

MitoNEET is a mammalian mitochondrial outer membrane iron-sulfur protein with a potential pharmacological and clinical target of pioglitazone, an insulin-sensitizer for the treatment of type II diabetes. In this study, we conducted the phenotypal analyses of a deletion strain and several mutant strains of a bacterial mitoNEET homolog (TthNEET) of Thermus thermophilus HB8 in comparison with the wild-type strain, and re-analyzed the available metabolome and microarray datasets of this thermophile. We also obtained the high-resolution X-ray diffraction datasets of mammalian mitoNEET in complex with several drug compounds.

Research Products

• [Journal Article] Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin (2012)
  • Author(s): Iwasaki, T., Fukazawa, R., Miyajima- Nakano, Y., Baldansuren, A., Matsushita, S., Lin, M. T., Gennis, R.B., Hasegawa, K., Kumasaka, T., Dikanov, S.A.
  • Journal Title: J. Am. Chem. Soc Volume: 134 Pages: 19731-19738
  • Peer Reviewed
• [Journal Article] Dissection of hydrogen bond interaction network around an iron-sulfur cluster by site-specific isotope labeling of hyperthermophilic archaeal Rieske-type ferredoxin (2012)
  • Author(s): Iwasaki, T., Fukazawa, R., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Lin, M. T., Gennis, R.B., Hasegawa, K., Kumasaka, T., Dikanov, S.A.
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Issue: 48 Pages: 19731-19738
  • DOI: 10.1021/ja308049u
  • Peer Reviewed
• [Presentation] 2D pulsed EPR analysis of histidine ligand residue(s) of the thermophile Rieske and mitoNEET type iron-sulfur proteins. (Mz76) (2013)
  • Author(s): Matsushita, S., Fukazawa, R., Iwasaki, T., Taguchi, A., Baldansuren, A., Dikanov, S.A.
  • Organizer: 16th International Conference on Bioinorganic Chemistry
  • Place of Presentation: Grenoble, France
• [Presentation] Application of 2D pulsed EPR (HYSCORE) in the structural analysis of hydrogen bond network around a biological iron-sulfur cluster. (Mz54) (2013)
  • Author(s): Iwasaki, T., Miyajima-Nakano, Y., Fukazawa, R., Hagiuda, E., Matsushita, S., Hasegawa, K., Kumasaka, T., Baldansuren, A., Dikanov, S.A.
  • Organizer: 16th International Conference on Bioinorganic Chemistry
  • Place of Presentation: Grenoble, France
• [Presentation] Expression host strains for selective isotope labeling of thermophile enzymes. (#AP.P 15) (2013)
  • Author(s): Fukazawa, R., Lin, M. T., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Dikanov, S.A., Gennis, R.B., Iwasaki, T.
  • Organizer: Thermophiles 2013, 12th International Meeting at the University of Regensburg
  • Place of Presentation: Regensburg, Germany
• [Presentation] Thermophile homolog of mitoNEET from Thermus thermophilus HB8. (#FT.O 02) (2013)
  • Author(s): Iwasaki, T., Hagiuda, E., Fukazawa, R., Hayashi-Iwasaki, Y., Oshima, T., Dikanov, S. A., Hasegawa, K., Kumasaka, T.
  • Organizer: Thermophiles 2013, 12th International Meeting at the University of Regensburg
  • Place of Presentation: Regensburg, Germany
• [Presentation] 2D pulsed EPR analysis of histidine ligand residue(s) of the thermophile Rieske and mitoNEET type iron-sulfur proteins. (2013)
  • Author(s): Matsushita, S., Fukazawa, R., Iwasaki, T., Taguchi, A., Baldansuren, A., and Dikanov, S. A.
  • Organizer: 16th International Conference on Bioinorganic Chemistry
  • Place of Presentation: Grenoble, France
• [Presentation] Application of 2D pulsed EPR (HYSCORE) in the structural analysis of hydrogen bond network around a biological iron-sulfur cluster. (2013)
  • Author(s): Iwasaki, T., Miyajima-Nakano, Y., Fukazawa, R., Hagiuda, E., Matsushita, S., Hasegawa, K., Kumasaka, T., Baldansuren, A., and Dikanov, S. A.
  • Organizer: 16th International Conference on Bioinorganic Chemistry
  • Place of Presentation: Grenoble, France
• [Presentation] Expression host strains for selective isotope labeling of thermophile enzymes. (2013)
  • Author(s): Fukazawa, R., Lin, M. T., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Dikanov, S. A., Gennis, R. B., and Iwasaki, T.
  • Organizer: Thermophiles 2013, 12th International Meeting at the University of Regensburg
  • Place of Presentation: Regensburg, Germany
• [Presentation] Thermophile homolog of mitoNEET from Thermus thermopiles HB8 (selected as oral presentation). (2013)
  • Author(s): Iwasaki, T., Hagiuda, E., Fukazawa, R., Hayashi-Iwasaki, Y., Oshima, T., Dikanov, S. A., Hasegawa, K., and Kumasaka, T.
  • Organizer: Thermophiles 2013, 12th International Meeting at the University of Regensburg
  • Place of Presentation: Regensburg, Germany
• [Presentation] 代謝系進化と補酵素・補欠分子族の関わり (2013)
  • Author(s): 岩崎 俊雄
  • Organizer: 電気通信大学シンポジウム「生命システム原材料の起源と進化: 生化学的禁制律」
  • Place of Presentation: 調布
  • Invited
• [Presentation] 生命進化と鉄硫黄クラスター : 蛋白質骨格による機能チューニング (2012)
  • Author(s): 岩崎俊雄
  • Organizer: 東京理科大学・基礎工学研究科・生物工学専攻TB院公開セミナー
  • Place of Presentation: 野田(野田キャンパス講義棟)
  • Year and Date: 2012-10-19
• [Presentation] 代謝系進化と補酵素・補欠分子族の関わり (2012)
  • Author(s): 岩崎俊雄
  • Organizer: 電気通信大学シンポジウム「生命システム原材料の起源と進化:生化学的禁制律」
  • Place of Presentation: 調布(電気通信大学B棟)
  • Year and Date: 2012-10-19
• [Presentation] Characterization of the extremophile homologs of mitoNEET. (#P117) (2012)
  • Author(s): Hagiuda, E., Miyajima-Nakano, Y., Ohmori, D., Kusano, T., Matsushita, S., Dikanov, S.A., Kumasaka, T., Iwasaki, T.
  • Organizer: 9th International Congress on Extremophiles
  • Place of Presentation: Sevilla, Spain
• [Presentation] Selective isotope labelling of extremophile metalloproteins. (#P119) (2012)
  • Author(s): Fukazawa, R., Lin, M.T., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Choi, S. K., Dikanov, S.A., Gennis, R.B., Iwasaki, T.
  • Organizer: 9th International Congress on Extremophiles
  • Place of Presentation: Sevilla, Spain
• [Presentation] Characterization of the extremophile homologs of mitoNEET (2012)
  • Author(s): Hagiuda, E., Miyajima-Nakano, Y., Ohmori, D., Kusano, T., Matsushita, S., Dikanov, S.A., Kumasaka, T., Iwasaki, T.
  • Organizer: 9th International Congress on Extremophiles
  • Place of Presentation: Sevilla, Spain
• [Presentation] Structural basis for metallosulfur recognition of archaeal Rieske protein scaffold (2012)
  • Author(s): Iwasaki, T., Kumasaka, T.
  • Organizer: 9th International Congress on Extremophiles
  • Place of Presentation: Sevilla, Spain
• [Presentation] Selective isotope labelling of extremophile metalloproteins (2012)
  • Author(s): Fukazawa, R., Lin, M. T., Miyajima-Nakano, Y., Baldansuren, A., Matsushita, S., Choi, S.K., Dikanov, S.A., Gennis, R.B., Iwasaki, T.
  • Organizer: 9th International Congress on Extremophiles
  • Place of Presentation: Sevilla, Spain
• [Presentation] Active site structure of hyperthermophilic archaeal Rieske-type ferredoxin (ARF) (2012)
  • Author(s): Hasegawa, K., Matsushita, S., Konosu, A., Baldansuren, A., Fukazawa, R., Dikanov, S.A., Iwasaki, T., Kumasaka, T.
  • Organizer: 9th International Congress on Extremophiles
  • Place of Presentation: Sevilla, Spain
• [Remarks]
  • URL: http://www.nms.ac.jp/fesworld/top.html
• [Remarks] 岩崎 俊雄 グループ ホームページ
  • URL: http://www.nms.ac.jp/fesworld/top.html