| Project/Area Number |
24659202
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUSANO Teruo 日本医科大学, 医学部, 助教 (30434129)
KUMASAKA Takashi 高輝度光科学研究センター, 利用研究促進部門, 副主席研究員 (30291066)
IWASAKI Hideo 早稲田大学, 理工学術院, 教授 (00324393)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | mitoNEET / 鉄硫黄クラスター / 金属蛋白質 / 構造機能生理 / 好熱菌 / シアノバクテリア / レドックス |
|---|
| Research Abstract |
MitoNEET is a mammalian mitochondrial outer membrane iron-sulfur protein with a potential pharmacological and clinical target of pioglitazone, an insulin-sensitizer for the treatment of type II diabetes. In this study, we conducted the phenotypal analyses of a deletion strain and several mutant strains of a bacterial mitoNEET homolog (TthNEET) of Thermus thermophilus HB8 in comparison with the wild-type strain, and re-analyzed the available metabolome and microarray datasets of this thermophile. We also obtained the high-resolution X-ray diffraction datasets of mammalian mitoNEET in complex with several drug compounds.
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