| Project/Area Number |
24659204
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
MORIISHI Kohji 山梨大学, 医学工学総合研究部, 教授 (90260273)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMASHITA Atsuya 山梨大学, 医学工学総合研究部, 助教 (00334871)
KASAI Hirotake 山梨大学, 医学工学総合研究部, 助教 (20324189)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | C型肝炎ウイルス / 人獣共通感染症 / 非霊長類ヘパシウイルス / フラビウイルス / ヘパシウイルス |
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| Research Abstract |
The objective of this study is to clarify if non-primate hepacivirus (NPHV) infects dogs or other animals in Japan. NPHV has been recently reported as a close homologue of hepatitis C virus (HCV). We prepared nose swabs from 11 dogs but did not find NPHV gene in those samples by RT-PCR. We also attempted to identify the virus from Japanese-born horses. Sera were prepared from 30 horses and were subjected to genetic analyses using RT-PCR. We identified equine hepacivirus (EHcV) in 30% preparations and determined a whole genome sequence. Non-translated regions and closed regions exhibit a similar structure to those of HCV, although 3'untranslated region of EHcV had not been reported. These results suggest that EHcV infects Japanese-born horses at a high rate in Japan compared to other countries.
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