Infection of hepatitis C virus-related non-primate hepacivirus in Japan and analysis on its replication mechanism
Project/Area Number |
24659204
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Virology
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Research Institution | University of Yamanashi |
Principal Investigator |
MORIISHI Kohji 山梨大学, 医学工学総合研究部, 教授 (90260273)
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Co-Investigator(Renkei-kenkyūsha) |
YAMASHITA Atsuya 山梨大学, 医学工学総合研究部, 助教 (00334871)
KASAI Hirotake 山梨大学, 医学工学総合研究部, 助教 (20324189)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | C型肝炎ウイルス / 人獣共通感染症 / 非霊長類ヘパシウイルス / フラビウイルス / ヘパシウイルス |
Research Abstract |
The objective of this study is to clarify if non-primate hepacivirus (NPHV) infects dogs or other animals in Japan. NPHV has been recently reported as a close homologue of hepatitis C virus (HCV). We prepared nose swabs from 11 dogs but did not find NPHV gene in those samples by RT-PCR. We also attempted to identify the virus from Japanese-born horses. Sera were prepared from 30 horses and were subjected to genetic analyses using RT-PCR. We identified equine hepacivirus (EHcV) in 30% preparations and determined a whole genome sequence. Non-translated regions and closed regions exhibit a similar structure to those of HCV, although 3'untranslated region of EHcV had not been reported. These results suggest that EHcV infects Japanese-born horses at a high rate in Japan compared to other countries.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Ca(2+) /S100 proteins regulate HCV virus NS5A-FKBP8/FKBP38 interaction and HCV virus RNA replication2013
Author(s)
Tani J, Shimamoto S, Mori K, Kato N, Moriishi K, Matsuura Y, Tokumitsu H, Tsuchiya M, Fujimoto T, Kato K, Miyoshi H, Masaki T, Kobayashi R.
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Journal Title
Liver Int
Volume: 33
Issue: 7
Pages: 1008-1018
DOI
Related Report
Peer Reviewed
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[Journal Article] Understanding the biological context of NS5A-host interactions in HCV infection: a network-based approach.2013
Author(s)
Tripathi LP, Kambara H, Chen YA, Nishimura Y, Moriishi K, Okamoto T, Morita E, Abe T, Mori Y, Matsuura Y, Mizuguchi K
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Journal Title
J Proteome Res
Volume: 12
Issue: 6
Pages: 2537-2551
DOI
Related Report
Peer Reviewed
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[Journal Article] Psammaplin A inhibits hepatitis C virus NS3 helicase2013
Author(s)
Salam, K.A.; Furuta, A.; Noda, N.; Tsuneda, S.; Sekiguchi, Y.; Yamashita, A.; Moriishi, K.; Nakakoshi, M.; Tsubuki, M.; Tani, H.; Tanaka, J.; Akimitsu, N
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Journal Title
J. Nat. Med. in press
Volume: 67
Issue: 4
Pages: 765-772
DOI
NAID
Related Report
Peer Reviewed
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[Presentation]2013
Author(s)
Tanaka T, Kasai H, Yamashita A, Moriishi K.
Organizer
20^<th> International Symposium on Hepatitis C virus and related viruses
Place of Presentation
Melbourne, Australia
Related Report
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[Presentation] FKBP6 plays an important role in HCV replication through binding to HCV NS5A2012
Author(s)
Kasai H., Kawakami, K., Yamashita, A., Ikeda, M., Kato, N., Enomoto, N., Matsuura, Y., Kusunoki, M., Moriishi, K.
Organizer
19^<th> International Symposium on Hepatitis C virus and related viruses
Place of Presentation
Venice, Italy
Related Report
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