Chromosomal instability and Clinico-pathologial feature in Lung Cancer

• Project/Area Number: 24659267
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: The University of Tokyo
• Principal Investigator: TAKAI Daiya 東京大学, 医学部附属病院, 講師 (90361493)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肺がん / ３'非翻訳領域 / マイクロＲＮＡ / 腫瘍検査学 / 発現制御

Research Abstract

We changed study design because several genes showed no mutation included in original study design. Thus we analyzed 3' untranslated region (3'UTR) of mRNA that was revealed to be shorten in cancers. 3'UTR is important in post transcriptional regulation of genes and it is known as a target of microRNAs. We firstly analyzed lung cancer specifically shorten mRNA using computational analyses of opened microarray data. We choose ten genes which are shortened in lung cancer and evaluated their status in clinical specimens using with quantitative RT-PCR. We revealed that increase of gene number of 3'UTR shortening is strongly associated post surgical recurrence in lung cancer.

Research Products

• [Journal Article] Combined Large Cell Neuroendocrine Carcinoma and Adenocarcinoma with Epidermal Growth Factor Receptor Mutation in a Female Patient Who Never Smoked (2014)
  • Author(s): Yoshida Y, Ota S, Murakawa T, Takai D
  • Journal Title: Annals of Thoracic and Cardiovascular Surgery Volume: 20 Issue: Supplement Pages: 582-584
  • DOI: 10.5761/atcs.cr.12.02217
  • NAID: 130003377861
  • ISSN: 1341-1098, 2186-1005
  • Peer Reviewed
• [Journal Article] Growth-associated hyperphosphatemia in young recipients accelerates aortic allograft calcification in a rat model (2013)
  • Author(s): Yamauchi H, Motomura N, Chung UI, Sata M, Takai D, Saito A, Ono M, Takamoto S.
  • Journal Title: J Thorac Car diovasc Surg Volume: 145(2) Pages: 522-530
• [Journal Article] Uridine diphosphate glucuronosyl transferase 1 family polypeptide A1 gene (UGT1A1) polymorphisms are associated with toxicity and efficacy in irinotecan monotherapy for refractory pancreatic cancer (2013)
  • Author(s): Takahara N, Nakai Y, Isayama H, Sasaki T, Satoh Y, Takai D, Hamada T, Uchino R, Mizuno S, Miyabayashi K, Mohri D, Kawakubo K, Kogure H, Yamamoto N, Sasahira N, Hirano K, Ijichi H, Tada M, Yatomi Y, Koike K.
  • Journal Title: Cancer Chemother Pharmacol Volume: 71(1) Pages: 85-92
• [Journal Article] High-grade lung adenocarcinoma with fetal-lung-like morphology–Clinicopathological, immunohistochemical, and molecular analyses of 17 cases (2013)
  • Author(s): Morita S, Yoshida A, Goto A, Ota S, Tsuta K, Yokozawa K, Asamura H, Nakajima J, Takai D, Mori M, Oka T, Tamaru J, Itoyama S, Furuta K, Fukayama M, Tsuda H.
  • Journal Title: Amer ican J of Sur g. Pathology Volume: 37(6) Pages: 924-932
• [Journal Article] FER overexpression is associated with poor postoperative prognosis and cancer-cell survival in non-small cell lung cancer (2013)
  • Author(s): Kawakami M, Morita S, Sunohara M, Amano Y, Ishikawa R, Watanabe K, Hamano E, Ohishi N, Nakajima J, Yatomi Y, Nagase T, Fukayama M, Takai D.
  • Journal Title: Int J Clin Exp Pathol Volume: 6(4) Pages: 598-612
• [Journal Article] A pilot study of salvage irinotecan monotherapy for advanced biliary tract cancer (2013)
  • Author(s): Sasaki T, Isayama H, Nakai Y, Takahara N, Satoh Y, Takai D, Kogure H, Yamamoto N, Hirano K, Tada M, Yatomi Y, Koike K.
  • Journal Title: Anticancer Res Volume: 33(6) Pages: 2619-2622
• [Journal Article] Combined Large Cell Neuroendocrine Carcinoma and Adenocarcinoma with Epidermal Growth Factor Receptor Mutation in a Female Patient Who Never Smoked (2013)
  • Author(s): Yoshida Y, Ota S, Murakawa T, Takai D, Nakajima J.
  • Journal Title: Ann Thorac Cardiovasc Sur g Volume: (Epub ahead)
  • NAID: 130003377861
• [Journal Article] Detection of novel paraja ring finger 2-fer tyrosine kinase mRNA chimeras is associated with poor postoperative prognosis in non-small cell lung cancer (2013)
  • Author(s): Kawakami M, Ishikawa R, Amano Y, Sunohara M, Watanabe K, Ohishi N, Yatomi Y, Nakajima J, Fukayama M, Nagase T, Takai D.
  • Journal Title: Cancer Sci Volume: 104(11) Pages: 1447-1454
• [Journal Article] Successful Treatment of Mediastinal Unicentric Castleman's Disease Using Video-Assisted Thoracoscopic Surgery with Preoperative Embolization (2013)
  • Author(s): Amano Y, Takai D, Ohishi N, Shinozaki-Ushiku A, Fukayama M, Akahane M, Nakajima J, Nagase T.
  • Journal Title: Case Rep Med Pages: 354507-354507
• [Journal Article] Disruption of the expression and function of microRNAs in lung cancer as a result of epigenetic changes (2013)
  • Author(s): Watanabe K & Takai D.
  • Journal Title: Frontier s in Genetics Volume: 4 Pages: 275-275
• [Journal Article] Disruption of the expression and function of microRNAs in lung cancer as a result of epigenetic changes. (2013)
  • Author(s): Watanabe K, Takai D.
  • Journal Title: Frontiers in Genetics Volume: 4 Pages: 275-275
  • Peer Reviewed
• [Journal Article] Successful Treatment of Mediastinal Unicentric Castleman's Disease Using Video-Assisted Thoracoscopic Surgery with Preoperative Embolization. (2013)
  • Author(s): Amano Y, Takai D, Ohishi N, Shinozaki-Ushiku A, Fukayama M, Akahane M, Nakajima J, Nagase T.
  • Journal Title: Case Reports in Medicine Volume: 2013 Pages: 354507-354507
  • DOI: 10.1155/2013/354507
  • Peer Reviewed
• [Journal Article] Detection of novel paraja ring finger 2-fer tyrosine kinase mRNA chimeras is associated with poor postoperative prognosis in non-small cell lung cancer. (2013)
  • Author(s): Kawakami M, Ishikawa R, Amano Y, Sunohara M, Watanabe K, Ohishi N, Yatomi Y, Nakajima J, Fukayama M, Nagase T, Takai D.
  • Journal Title: Cancer Sci Volume: 104 Issue: 11 Pages: 1447-1454
  • DOI: 10.1111/cas.12250
  • Peer Reviewed
• [Journal Article] A pilot study of salvage irinotecan monotherapy for advanced biliary tract cancer. (2013)
  • Author(s): Sasaki T, Isayama H, Nakai Y, Takahara N, Satoh Y, Takai D, Kogure H, Yamamoto N, Hirano K, Tada M, Yatomi Y, Koike K.
  • Journal Title: Anticancer Research Volume: 33 Pages: 2619-2622
  • Peer Reviewed
• [Journal Article] High-grade Lung Adenocarcinoma With Fetal Lung-like Morphology: Clinicopathologic, Immunohistochemical, and Molecular Analyses of 17 Cases (2013)
  • Author(s): Morita S, Yoshida A, Goto A, Ota S, Tsuta K, Yokozawa K, Asamura H, Nakajima J, Takai D, Mori M, Oka T, Tamaru J, Itoyama S, Furuta K, Fukayama M, Tsuda H.
  • Journal Title: Am J Surg Pathol Volume: 37(6) Issue: 6 Pages: 924-932
  • DOI: 10.1097/pas.0b013e31827e1e83
  • Peer Reviewed
• [Journal Article] FER overexpression is associated with poor postoperative prognosis and cancer-cell survival in non-small cell lung cancer. (2013)
  • Author(s): Kawakami M, Morita S, Sunohara M, Amano Y, Ishikawa R, Watanabe K, Hamano E, Ohishi N, Nakajima J, Yatomi Y, Nagase T, Fukayama M, Takai D.
  • Journal Title: International Journal of Clinical & Experimental Pathology Volume: 6 Pages: 598-612
  • Peer Reviewed
• [Journal Article] Combined Large Cell Neuroendocrine Carcinoma and Adenocarcinoma with Epidermal Growth Factor Receptor Mutation in a Female Patient Who Never Smoked. (2013)
  • Author(s): Yoshida Y, Ota S, Murakawa T, Takai D, Nakajima J.
  • Journal Title: Annals of Thoracic and Cardiovascular Surgery Volume: Epub ahead
  • NAID: 130003377861
  • Peer Reviewed
• [Journal Article] Growth-associated hyperphosphatemia in young recipients accelerates aortic allograft calcification in a rat model. (2013)
  • Author(s): Yamauchi Haruo
  • Journal Title: The Journal of Thoracic and Cardiovascular Surgery Volume: 145 Issue: 2 Pages: 522-530
  • DOI: 10.1016/j.jtcvs.2012.03.010
  • Peer Reviewed
• [Journal Article] Uridine diphosphate glucuronosyl transferase 1 family polypeptide A1 gene (UGT1A1) polymorphisms are associated with toxicity and efficacy in irinotecan monotherapy for refractory pancreatic cancer (2013)
  • Author(s): Takahara N, Nakai Y, Isayama H, Sasaki T, Satoh Y, Takai D, Miyabayashi K, Mohri D, Kawakubo K, Kogure H, Yamamoto N, Sasahira N, Hirano K, Ijichi H, Tada M, Yatomi Y, Koike K
  • Journal Title: Cancer Chemotherapy and Pharmacology Volume: 71 Issue: 1 Pages: 85-92
  • DOI: 10.1007/s00280-012-1981-0
  • Peer Reviewed
• [Journal Article] High-grade lung adenocarcinoma with fetal-lung-like morphology ? Clinicopathological, immunohistochemical, and molecular analyses of 17 cases (2013)
  • Author(s): Morita S, Yoshida A, Goto A, Ota S, Tsuta K, Yokozawa K, Asamura H, Nakajima J, Takai D, Mori M, Oka T, Tamaru J, Itoyama S, Furuta K, Fukayama M, Tsuda H
  • Journal Title: American Journal of Surgical Pathology Volume: in press
  • Peer Reviewed
• [Journal Article] FER overexpression is associated with poor postoperative prognosis and cancer-cell survival in nonsmall cell lung cancer (2013)
  • Author(s): Masanori Kawakami, Shigeki Morita, Mitsuhiro Sunohara, Yosuke Amano, Rie Ishikawa, Kousuke Watanabe, Emi Hamano, Nobuya Ohishi,Yutaka Yatomi, Takahide Nagase, Masashi Fukayama, Daiya Takai
  • Journal Title: International Journal of Clinical and Experimental Pathology Volume: 6 Pages: 598-612
  • Peer Reviewed
• [Journal Article] Genome structure-based screening identified epigenetically silenced microRNA associated with invasiveness in non-small-cell lung cancer (2012)
  • Author(s): Watanabe K, Emoto N, Hamano E, Sunohara M, Kawakami M, Kage H, Kitano K, Nakajima J, Goto A, Fukayama M, Nagase T, Yatomi Y, Ohishi N, Takai D.
  • Journal Title: Int J Cancer Volume: 130(11) Pages: 2580-2590
• [Journal Article] Pulmonary Venous Invasion, Determined by Chest Computed Tomographic Scan, as a Potential Early Indicator of Zygomycosis Infection : A Case Series (2012)
  • Author(s): Kitagawa H, Watanabe K, Kage H, Inoh S, Goto A, Fukayama M, Nagase T, Ohishi N, Takai D.
  • Journal Title: J Thorac Imaging Volume: 27(4)
• [Journal Article] Analysis for the combination expression of CK20, FABP1 and MUC2 is sensitive for the prediction of peritoneal recurrence in gastric cancer (2012)
  • Author(s): Satoh Y, Mori K, Kitano K, Kitayama J, Yokota H, Sasaki H, Uozaki H, Fukayama M, Seto Y, Nagawa H, Yatomi Y, Takai D.
  • Journal Title: J pn J Clin Oncol Volume: 42(2) Pages: 148-152
• [Journal Article] Genome structure-based screening identifiedepigenetically silenced microRNA associated with invasiveness in non-small-cell lung cancer. (2012)
  • Author(s): Watanabe K, Goto A, Takai D.
  • Journal Title: Int J Cancer. Volume: 130 Issue: 11 Pages: 2580-2590
  • DOI: 10.1002/ijc.26254
  • Peer Reviewed
• [Journal Article] Pulmonary Venous Invasion, Determined by Chest Computed Tomographic Scan, as a Potential Early Indicator of Zygomycosis Infection: A Case Series (2012)
  • Author(s): Kitagawa H, Watanabe K, Kage H, Inoh S, Goto A, Fukayama M, Nagase T, Ohishi N, Takai D
  • Journal Title: J Thorac Imaging Volume: 27 Issue: 4 Pages: W97-W99
  • DOI: 10.1097/rti.0b013e3182282d17
  • Peer Reviewed
• [Journal Article] Analysis for the combination expression of CK20, FABP1 and MUC2 is sensitive for the prediction of peritoneal recurrence in gastric cancer (2012)
  • Author(s): Satoh Y, Mori K, Kitano K, Kitayama J, Yokota H, Sasaki H, Uozaki H, Fukayama M, Seto Y, Nagawa H, Yatomi Y, Takai D
  • Journal Title: Jpnease Journal of Clinical Oncology Volume: 42 Issue: 2 Pages: 148-152
  • DOI: 10.1093/jjco/hyr179
  • Peer Reviewed
• [Presentation] Loss of PABPN1 and alternative polyadenylation yield poor prognosis in non-small cell lung cancer (2014)
  • Author(s): Ichinose J, Watanabe K, Sano A, Nagase T, Nakajima J, Fukayama M, Yatomi Y, Ohishi N, Takai D.
  • Organizer: AACR-IASLC Joint Conference on The Molecular Origins of Lung Cancer
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Can lung cancer with overexpression of RET-proto oncogene be a good candidate for vandetanib treatment? (2014)
  • Author(s): Sunohara M, Morita S, Kawakami M, Watanabe K, Kage H, Amano Y, Ishikawa R, Fukayama M, Nagase T, Ohishi N, Takai D.
  • Organizer: AACR-IASLC Joint Conference on The Molecular Origins of Lung Cancer
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Histone methylation-mediated silencing of mir-139 enhances an aggressive phenotype of non-small cell lung cancer (2014)
  • Author(s): Watanabe K, Sunohara M, Amano Y, Ishikawa R, Ichinose J, Nakajima J, Fukayama M, Yatomi Y, Nagase T, Ohishi N, Takai D.
  • Organizer: AACR-IASLC Joint Conference on The Molecular Origins of Lung Cancer
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Loss of PABPN1 and alternative polyadenylation yield poor prognosis in non-small cell lung cancer. (2014)
  • Author(s): Ichinose J, Watanabe K, Sano A, Nagase T, Nakajima J, Fukayama M, Yatomi Y, Ohishi N, Takai D
  • Organizer: AACR-IASLC Joint Conference on The Molecular Origins of Lung Cancer
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Can lung cancer with overexpression of RET-proto oncogene be a good candidate for vandetanib treatment ? (2014)
  • Author(s): Sunohara M, Morita S, Kawakami M, Watanabe K, Kage H, Amano Y, Ishikawa R, Fukayama M, Nagase T, Ohishi N, Takai D
  • Organizer: AACR-IASLC Joint Conference on The Molecular Origins of Lung Cancer
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Histone methylation-mediated silencing of mir-139 enhances an aggressive phenotype of non-small cell lung cancer. (2014)
  • Author(s): Watanabe K, Sunohara M, Amano Y, Ishikawa R, Ichinose J, Nakajima J, Fukayama M, Yatomi Y, Nagase T, Ohishi N, Takai D
  • Organizer: AACR-IASLC Joint Conference on The Molecular Origins of Lung Cancer
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Vandetanib Can Overcome the Acquired Resistance to Gefitinib Due to RET Overexpression in Lung Cancer (2012)
  • Author(s): Sunohara M, Kawakami K, Watanabe K, Morita S, Kage H, Amano Y , Fukayama M, Nagase T, N. Ohishi N, Takai D.
  • Organizer: The 24th EORTC-NCI-AACR Symposium on'Molecular Targets and Cancer Therapeutics'
  • Place of Presentation: Dublin, Island
• [Presentation] Identification of FER as a New Molecular Target in Non-small Cell Lung Cancer (2012)
  • Author(s): Kawakami M, Sunohara M, Amano Y, Ishikawa R, Watanabe K, Hamano E, Ohishi N, Nagase T, Yatomi Y, Takai D.
  • Organizer: The 24th EORTC-NCI- AACR Symposium on'Molecular Targets and Cancer Therapeutics'
  • Place of Presentation: Dublin, Island
• [Presentation] Vandetanib Can Overcome the Aquired Resistance to Gefitinib Due to RET Overexpression in Lung Cancer (2012)
  • Author(s): M. Sunohara, M. Kawakami, K. Watanabe, S. Morita, H. Kage, Y. Amano, M. Fukayama, T. Nagase, N. Ohishi, D. Takai
  • Organizer: The 24th EORTC-NCI-AACR Symposium on ‘Molecular Targets and Cancer Therapeutics'
  • Place of Presentation: Dublin, Ireland
• [Presentation] Identification of FER as a New Molecular Target in Non-small Cell Lung Cancer (2012)
  • Author(s): M. Kawakami, M. Sunohara, Y. Amano, R. Ishikawa, K. Watanabe, E. Hamano, N. Ohishi, T. Nagase, Y. Yatomi, D. Takai
  • Organizer: The 24th EORTC-NCI-AACR Symposium on ‘Molecular Targets and Cancer Therapeutics'
  • Place of Presentation: Dublin, Ireland
• [Remarks]
  • URL: http://kokyuki.umin.jp/305.html