| Project/Area Number |
24659333
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Public health/Health science
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AOYAMA Ikuko 大阪府立公衆衛生研究所, その他部局等, 研究員 (90332452)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Shota (90432434)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 蚊 / ウイルス / 不明熱 / 中和抗体 / 公衆衛生 |
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| Research Abstract |
In this study, we tried the analysis of the unknown virus isolated from a patient with persistent high fever by using a C6/36 mosquito cell line. As the results, we confirmed the proliferation of viruses in cultured mosquito cells and the capability of forming plaques. We could not confirm the pathogenicity in mice, although the propagation in a mammalian cell line (mouse glioma F10) was observed. An IgM antibody was also detected in the serum of the patient. The observation with the transmission electron microscope revealed two kinds of viral particles (spindle or polyhedron-like shape). In the genetic analysis, we determined 5,263 nt sequences of the viral genome and BLAST analysis suggested the novelty of the isolated virus.
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