| Project/Area Number |
24659340
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Tokai University |
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Principal Investigator |
OSAWA Motoki 東海大学, 医学部, 教授 (90213686)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Fumiko 東海大学, 医学部, 准教授 (70328128)
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| Co-Investigator(Renkei-kenkyūsha) |
HASAGAWA Iwao 東海大学, 医学部, 助教 (00433912)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 循環器 / 石灰化 / 動脈 / ゲノム / 病理学 |
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| Research Abstract |
Arterial calcification is the basic lesion in which aortic dissection and ischemic heart diseases are developed. In the present study, we attempted to establish the histological evaluation procedure of the ectopic calcification. Laboratory staining and immunohistochemistry were performed to sections of paraffin-embedded aorta tissues obtained at autopsy. As the classification, five stages were evident such as diffuse apatite particles, massive calcification accompanied by atheroma, and nucleus or marrow formation. The calcification pattern was not consistent with atherosclerotic changes according to grades of the American Heart Association. In particular, advanced subjects displaying V and VI types exhibited a variety of calcification features. In addition to the common H&E staining, Kossa staining should be suitable at laboratory for evaluation of calcification in the sections. If necessary, immunohistochemistry using anti-MGP and anti-AHSG antibodies should be employed.
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