A novel ultrasound imaging for pancreatic cancer using tumor directed-contrast media

• Project/Area Number: 24659372
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Sapporo Medical University
• Principal Investigator: KATO Junji 札幌医科大学, 医学部, 教授 (20244345)
• Co-Investigator(Kenkyū-buntansha): TAKIMOTO Rishu 札幌医科大学, 医学部, 准教授 (10336399) ; HAYASHI Tsuyoshi 札幌医科大学, 医学部, 講師 (60381258) ; ISHIWATARI Hirotoshi 札幌医科大学, 医学部, 助教 (90468083)
• Project Period (FY): 2012 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 超音波造影剤 / 膵癌 / フコース / ナノキャリア / ペルフルブタン / リポソーム

Research Abstract

Patients with pancreatic ductal carcinoma (PDAC) are dismal prognosis due to absence of effective modality to diagnose at early stage. We have recently developed a novel targeting nanoparticle for PDAC using fucose receptor mediated-endocytosis (PLoS ONE, 2012). Our nanoparticles carrying anti-cancer drugs could specifically delivered agents to PDAC cells and suppressed tumor growth. We then utilized this strategy for diagnosing PDAC as a novel ultrasound imaging. We encapsulated perfulbutane (PFB) into our nanoparticles and succeeded to image PDAC as a tumor in xenografted rat model using ultrasound sonography.

Research Products

• [Journal Article] Fucosylated TGF-β receptors transduces a signal for epithelialmesenchymal transition in colorectal cancer cells. (2014)
  • Author(s): Hirakawa M, Takimoto R, Hayashi T, Kobune M, Kato J, et al.
  • Journal Title: Br J Cancer Volume: 110 Pages: 156-63
  • Peer Reviewed
• [Journal Article] A novel strategy inducing autophagic cell death in Burkitt's lymphoma cells with anti-CD19-targeted liposomal rapamycin. (2014)
  • Author(s): Ono K, Hayashi T, Miyanishi K, Sato Y, Takimoto R, Kobune M, Kato J, et al.
  • Journal Title: Blood Cancer J Volume: 7
  • Peer Reviewed
• [Journal Article] Fucosylated TGF-ß receptors transduces a signal for epithelial-mesenchymal transition in colorectal cancer cells. (2014)
  • Author(s): Hirakawa M, Takimoto R, Tamura F, Yoshida M, Ono M, Murase K, Sato Y, Osuga T, Sato T, Iyama S, Miyanishi K, Takada K, Hayashi T, Kobune M, Kato J.
  • Journal Title: Br J Cancer Volume: 110 Issue: 1 Pages: 156-163
  • DOI: 10.1038/bjc.2013.699
  • Peer Reviewed / Open Access
• [Journal Article] A novel strategy inducing autophagic cell death in Burkitt’s lymphoma cells with anti-CD19-targeted liposomal rapamycin. (2014)
  • Author(s): Ono K, Sato T, Iyama S, Tatekoshi A, Hashimoto A, Kamihara Y, Horiguchi H, Kikuchi S, Kawano Y,Takada K, Hayashi T, Miyanishi K, Sato Y, Takimoto R, Kobune M, Kato J.
  • Journal Title: Blood Cancer J Volume: 7 Issue: 2 Pages: e180-e180
  • DOI: 10.1038/bcj.2014.2
  • Peer Reviewed / Open Access
• [Journal Article] A phase II study of neoadjuvant combination chemotherapy with docetaxel, cisplatin, and S-1 for locally advanced resectable gastric cancer : nucleotide excision repair (NER) as potential chemoresistance marker. (2013)
  • Author(s): Hirakawa M, Hayashi T , Sato T ,Miyanishi K,Takimoto R,Kobune M,Hirata K,Kato J, et al.
  • Journal Title: Cancer Chemother Pharmacol Volume: 71 Pages: 789-797
  • Peer Reviewed
• [Journal Article] Increased hepatic oxidative DNA damage in patients with nonalcoholic steatohepatitis who develop hepatocellular carcinoma. (2013)
  • Author(s): Tanaka S, Hayashi T,Sato T,Sato Y,Takimoto R,Kato J, et al.
  • Journal Title: J Gastroenterol Volume: 28 Pages: 656-663
  • Peer Reviewed
• [Journal Article] A case of idiopathic encapsulating peritoneal sclerosis with intractable ileus successfully treated by surgery and steroid therapy. (2013)
  • Author(s): Ikeda Y, Hayashi T,Sato T,Miyanishi K,Takimoto R,Kohune M,Nobuoka T,Noguchi H,Oi M,Honma H,Hirata K,Hasegawa T,Kato J, et al.
  • Journal Title: Clin J Gastroenterol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Rapid on-site evaluation by endosonographer during endoscopic ultrasound-guided fine needle aspiration for pancreatic solid masses. (2013)
  • Author(s): Hayashi T, Ishiwatari H, Takimoto R, Mitsuhashi T, Asanuma H, Ogino J, Hasegawa T, Sonoda T, Kato J, et al.
  • Journal Title: J Gastroenterol Hepatol Volume: 28 Pages: 656-663
  • Peer Reviewed
• [Journal Article] Treatment of pancreatic fibrosis with siRNA against a collagen-specific chaperone in vitamin A-coupled liposomes. (2013)
  • Author(s): Ishiwatari H, Takimoto R, Kato J, Niitsu Y, et al.
  • Journal Title: Gut Volume: 62 Pages: 1328-39
  • Peer Reviewed
• [Journal Article] Diagnostic validity of CT gastrography versus gastroscopy for primary lesions in gastric cancer : evaluating the response to chemotherapy, a retrospective analysis. (2013)
  • Author(s): Takahashi S, Takimoto R, Kobune M, Kato J, et al.
  • Journal Title: Gastric Cancer Volume: 16 Pages: 543-548
  • Peer Reviewed
• [Journal Article] A retrospective clinical analysis of Japanese patients with peripheral T-cell lymphoma not otherwise specified : Hokkaido Hematology Study Group. (2013)
  • Author(s): Torimoto Y, Takimoto R, Tanaka J, Yamamoto S, Kohgo Y, Fukuhara T, et al.
  • Journal Title: Int J Hematol Volume: 98 Pages: 171-8
  • Peer Reviewed
• [Journal Article] Treatment of pancreatic fibrosis with siRNA against a collagen-specific chaperone in vitamin A-coupled liposomes. (2013)
  • Author(s): Ishiwatari H, Sato Y, Murase K, Yoneda A, Fujita R, Nishita H, Birukawa NK, Hayashi T, Sato T, Miyanishi K, Takimoto R, Kobune M, Ota S, Kimura Y, Hirata K, Kato J, Niitsu Y.
  • Journal Title: Gut Volume: 62(9) Issue: 9 Pages: 1328-39
  • DOI: 10.1136/gutjnl-2011-301746
  • Peer Reviewed
• [Journal Article] A phase II study of neoadjuvant combination chemotherapy with docetaxel, cisplatin, and S-1 for locally advanced resectable gastric cancer: nucleotide excision repair (NER) as potential chemoresistance marker. (2013)
  • Author(s): Hirakawa M, Sato Y, Ohnuma H, Takayama T, Sagawa T, Nobuoka T, Harada K, Miyamoto H, Sato Y, Takahashi Y, Katsuki S, Hirayama M, Takahashi M, Ono M, Maeda M, Takada K, Hayashi T, Sato T, Miyanishi K, Takimoto R, Kobune M, Hirata K, Kato J
  • Journal Title: Cancer Chemother Pharmacol Volume: 71 Issue: 3 Pages: 789-797
  • DOI: 10.1007/s00280-013-2073-5
  • Peer Reviewed
• [Journal Article] Rapid on-site evaluation by endosonographer during endoscopic ultrasound-guided fine needle aspiration for pancreatic solid masses. (2013)
  • Author(s): Hayashi T, Ishiwatari H, Yoshida M, Ono M, Sato T, Miyanishi K, Sato Y, Kobune M, Takimoto R, Kato J, et al.
  • Journal Title: J Gastroenterol Hepatol. Volume: 28 Issue: 4 Pages: 656-663
  • DOI: 10.1111/jgh.12122
  • Peer Reviewed
• [Journal Article] Epidemiology and treatment outcome of invasive fungal infections in patients with hematological malignancies. (2012)
  • Author(s): Kurosawa M, Takimoto R, Torimoto Y, Mori A, Takahashi T, Iizuka S, Ishida T, Kobayashi R, Oda T, Sakai H, Yamamoto S, Takahashi F, Fukuhara T, et al.
  • Journal Title: Int J Hematol Volume: 96 Pages: 748-57
  • Peer Reviewed
• [Journal Article] Targeting anticancer drug delivery to pancreatic cancer cells using a fucose-bound nanoparticle approach. (2012)
  • Author(s): Yoshida M, Takimoto R, Murase K, Sato Y, Hirakawa M, Tamura F, Sato T, Iyama S, Osuga T, Miyanishi K, Takada K, Hayashi T, Kobune M, Kato J.
  • Journal Title: PLoS One Volume: 7(7) Issue: 7 Pages: e39545-e39545
  • DOI: 10.1371/journal.pone.0039545
  • Peer Reviewed
• [Journal Article] Prognostic significance of serum-ferritin level at diagnosis in myelodysplastic syndrome (2012)
  • Author(s): Kobune M, et al. 2^<nd>
  • Journal Title: Int J Hematol Volume: (In press) Issue: 5 Pages: 527-534
  • DOI: 10.1007/s12185-012-1048-3
  • NAID: 10030633185
  • Peer Reviewed
• [Presentation] Targeting Pancreatic Cancer Stem Like Cells Using a Nanoparticle Modified by L-Fucose. (2013)
  • Author(s): Takimoto R,Yoshida M,Murase K,Sato Y,Hirakawa M,Tamura F,Sato T,Osuga T,Miyanishi K,Takada K,Hayash T,Kobune M,Kato J.
  • Organizer: The American Association for the Study of Liver Diseases-Digestive Disease Week (DDW 2013)
  • Place of Presentation: Orlando, U.S.A.
• [Presentation] Iron chelation therapy improves oxidative DNA damage in hematopoietic cells derived from transfusion-dependent myelodysplastic syndrome. (2013)
  • Author(s): Kobune M,Kikuchi S,Iyama S,Takada K,Murase K,Ono K,Horiguchi H,Kamihara Y,Hayashi T,Sato T,Miyanishi K,Sato Y,Takimoto R,Kato J .
  • Organizer: Fifth Congress of the International BioIron Society (IBIS) Biennial World Meeting (BioIron2013)
  • Place of Presentation: London, UK.
• [Presentation] Targeting anticancer drug delivery to pancreatic cancer cells using a fucose-bound nanoparticle approach. (2013)
  • Author(s): Takimoto R, Yoshida M, Sato Y, Hirakawa M, Tamura F, Sato T, Iyama S, Osuga T, Miyanishi K, Takada K, Hayashi T, Kobune M, Kato J.
  • Organizer: Ninth AACR-Japanese Cancer Association Joint Conference : Breakthroughs in Basic and Translational Cancer Research
  • Place of Presentation: Maui,USA.
• [Presentation] Targeting Pancreatic Cancer Stem Like Cells Using a Nanoparticle Modified by L-Fucose． (2013)
  • Author(s): Takimoto R，Yoshida M，Murase K，Sato Y，Hirakawa M，Tamura F，Sato T，Osuga T，Miyanishi K，Takada K，Hayash T，Kobune M，Kato J．
  • Organizer: The American Association for the Study of Liver Diseases－Digestive Disease Week
  • Place of Presentation: Orlando, U.S.A