Role of INTS6 in the adipose differentiation
| Project/Area Number |
24659445
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Metabolomics
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ＩＮＴＳ６ / 脂肪細胞 / 代謝 / 分化 / 肥満 / INTS6 / 糖尿病 / 代謝調節 / インスリン抵抗性 |
|---|
| Research Abstract |
We demonstrated that expression levels ofIntS6 and IntS11, subunits ofthe Integrator complex, were increased in 3T3-L1 cells in the period when the cells reach edconfluenceand differentiated into adipocytes, while being reduced tobasal levels after the completion ofdifferentiation. Suppre ssion of IntS6 or IntS11 expression using siRNAs in3T3-L1 pre-adipocytes markedly inhibited differentiation into mature adipocytes. These findings demonstrate that increased expression of Integrator complex subunits is an indispensable event in adipose differentiation. Although further study is necessary to elucidate the underlying mechanism, the processing of U1, U2 small nuclear RNAs may be involved in cell differentiation steps.
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Report
(3 results)
Research Products
(75 results)
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Hashikata A, Yamashita A, Suzuki S, Nagayasu S, Shinjo T, Taniguchi A, Fukushima M, Nakai Y, Nin K, Watanabe N, Asano T, Abiko Y, Kushiyama A, Nagasaka S, Nishimura F.
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Nephrol Dial Transplant.
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Okubo H, Sakoda H, Kushiyama A, Fujishiro M, Nakatsu Y, Fukushima T, Matsunaga Y, Kamata H, Asahara T, Yoshida Y, Chonan O, Iwashita M, Nishimura F, Asano T.
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Am J Physiol Gastrointest Liver Physiol.
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[Journal Article] Par14 protein associates with insulin receptor substrate 1 (IRS-1), thereby enhancing insulin-induced IRS-1 phosphorylation and metabolic actions.2013
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Zhang J, Nakatsu Y, Shinjo T, Guo Y, Sakoda H, Yamamotoya T, Otani Y, Okubo H, Kushiyama A, Fujishiro M, Fukushima T, Tsuchiya Y, Kamata H, Iwashita M, Nishimura F, Katagiri H, Takahashi S, Kurihara H, Uchida T, Asano T.
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J Biol Chem.
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[Journal Article] Resistin-like molecule β is abundantly expressed in foam cells and is involved in atherosclerosis development.2013
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Kushiyama A, Sakoda H, Oue N, Okubo M, Nakatsu Y, Ono H, Fukushima T, Kamata H, Nishimura F, Kikuchi T, Fujishiro M, Nishiyama K, Aburatani H, Kushiyama S, Iizuka M, Taki N, Encinas J, Sentani K, Ogonuki N, Ogura A, Kawazu S, Yasui W, Higashi Y, Kurihara H, Katagiri H, Asano T.
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[Journal Article] Valsartan restores inflammatory response by macrophages in adipose and hepatic tissues of LPS-infused mice.2013
Author(s)
Iwashita M, Nakatsu Y, Sakoda H, Fujishiro M, Kushiyama A, Fukushima T, Kumamoto S, Shinjo T, Kamata H, Nishimura F, Asano T.
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Journal Title
Adipocyte
Volume: 2
Pages: 28-32
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[Journal Article] Integrator complex plays an essential role in adipose differentiation.2013
Author(s)
Otani Y, Nakatsu Y, Sakoda H, Fukushima T, Fujishiro M, Kushiyama A, Okubo H, Tsuchiya Y, Ohno H, Takahashi S, Nishimura F, Kamata H, Katagiri H, Asano T.
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Biochem Biophys Res Commun.
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[Journal Article] Valsartan, independently of AT1 receptor or PPAR, suppresses LPS-induced macrophage activation and improves insulin resistance in co-cultured adipocytes2012
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Iwashita, M., Sakoda, H., ,Nakatsu, Y., Asano, T.
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Am. J. Physiol. Endo. Metab
Volume: 302
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