A novel regulatory mechanism of blood glucose level in hepatocytes independent of hormonal control and its pharmacological application

Research Project

Project/Area Number	24659448
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Metabolomics
Research Institution	Osaka Medical College
Principal Investigator	WATANABE Fusao 大阪医科大学, 医学部, 准教授 (40183719)
Project Period (FY)	2012-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	グルコキナーゼ / グルコキナーゼ調節蛋白質 / 核‐細胞質間蛋白質輸送 / 核-細胞質間蛋白資質輸送 / 核-細胞質間蛋白質輸送 / グルコースセンサー / glucokinase / 細胞内局在性 / 肝細胞 / HepG2 / HEK293 / GKRP
Outline of Final Research Achievements	In response to hypoglycemia and hyperglycemia, hepatic glucokinase (GK) is localized to the nucleus and the cytoplasm, respectively. The intracellular localization of GK regulates the blood glucose level. In this study, I elucidate that the glucose sensing system or the GK transport mechanism in hepatocytes is impaired in HEK293 cells derived from human kidney. Moreover, antibodies were raised against the proteins involved in GK transport to clarify the protein-protein interaction with GK