| Project/Area Number |
24659455
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Endocrinology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | FXYD5 / Dysadherin / 甲状腺癌 / Eカドヘリン / 5XDYD |
|---|
| Research Abstract |
FXYD5 (Dysadherin) is a cancer-associated cell membrane glycoprotein and believed to down-regulate the expression of E-cadherin, the prime mediator of cell-cell adhesion in epithelial cells, by a post-transcriptional mechanism and to promote the metastasis of carcinoma cells. To evaluate the proteins associated with FXYD5, transfection and RNA interference (RNAi) of FXYD5 were performed in thyroid carcinoma cell lines. 10 clones of FXYD5 knocked down were gained. Features of the clones were morphologically changed epithelial, and migration potency was promoted. Proteomics analysis was performed, using the 10 clones. It was revealed that S-100 protein and RAS protein were overexpressed. The results indicated that FXYD5 was associated with migration potential and down-regulation of S-100 protein and RAS protein in the thyroid carcinoma.
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