Cancer stem cell assay by a new immunodeficient mouse model harboring a complete macrophage tolerance
| Project/Area Number |
24659463
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kyushu University |
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Principal Investigator |
AKASHI KOICHI 九州大学, 医学(系)研究科(研究院), 教授 (80380385)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | がん幹細胞 / 異種移植アッセイ / マクロファージ / SIRPA / 異種移植モデル / 免疫不全マウス / マクロファージ寛容 / CD47 |
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| Outline of Final Research Achievements |
To evaluate human cancer stem cells in xenotransplant models, a variety of immunodeficient mouse lines have been developed. We found that in xenograft engraftment, inhibition of phagocytic reaction of mouse macrophages is essential in addition to lymphoid depletion. Murine signal regulatory protein alpha (mSIRPA) on macrophages cannot bind to human CD47 (hCD47), resulting phagocytic reaction against human cells. Here, we have established a new immunodeficient BRGhS mouse line, B6.Rag2(null)Il2rg(null), harboring a complete macrophage tolerance. BRGhS mouse line was introduced by human SIRPA, which allows to bind human CD47, resulting in inhibition of phagocytosis against human cell graft. BRGhS strain should be very useful in future xenotransplant experiments of human cancer stem cells.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor.2015
Author(s)
Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, Shima T, Kikushige Y, Takenaka K, Miyamoto T, Akashi K.
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Journal Title
Stem Cells
Volume: 33
Issue: 3
Pages: 976-87
DOI
Related Report
Peer Reviewed
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[Journal Article] The ordered acquisition of Class II and Class I mutations directs formation of human t(8;21) acute myelogenous leukemia stem cell.2014
Author(s)
Shima T, Miyamoto T, Kikushige Y, Yuda J, Tochigi T, Yoshimoto G, Kato K, Takenaka K, Iwasaki H, Mizuno S, Goto N, Akashi K.
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Journal Title
Exp. Hematol.
Volume: 42
Issue: 11
Pages: 955-65
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Contribution of bone marrow-derived hematopoietic stem/progenitor cells to the generation of donor-marker(+) cardiomyocytes in vivo.2013
Author(s)
Fukata M, Ishikawa F, Najima Y, Yamauchi T, Saito Y, Takenaka K, Miyawaki K, Shimazu H, Shimoda K, Kanemaru T, Nakamura K, Odashiro K, Nagafuji K, Harada M, Akashi K.
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Journal Title
PLoS One
Volume: 8
Issue: 5
Pages: e62506-e62506
DOI
Related Report
Peer Reviewed
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[Journal Article] Reciprocal expression of enteric antimicrobial proteins in intestinal graft-versus-host disease.2013
Author(s)
Eriguchi Y, Uryu H, Nakamura K, Shimoji S, Takashima S, Iwasaki H, Miyamoto T, Shimono N, Hashimoto D, Akashi K, Ayabe T, Teshima T.
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Journal Title
Biol Blood Marrow Transplant
Volume: 19
Issue: 10
Pages: 1525-1529
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Monitoring of minimal residual disease (MRD) is useful to predict prognosis of adult patients with Ph-negative ALL : results of a prospective study (ALL MRD2002 Study)2013
Author(s)
Nagafuji K, Miyamoto T, Eto T, Kamimura T, Taniguchi S, Okamura T, Ohtsuka E, Yoshida T, Higuchi M, Yoshimoto G, Fujisaki T, Abe Y, Takamatsu Y, Yokota S, Akashi K, Harada M. (16名, 6番目)
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Journal Title
J Hematol Oncol
Volume: 6
Issue: 1
Pages: 14-14
DOI
Related Report
Peer Reviewed
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[Presentation] 造血器腫瘍幹細胞2014
Author(s)
赤司 浩一
Organizer
第101回近畿血液学地方会
Place of Presentation
大阪
Year and Date
2014-06-28
Related Report
Invited
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[Presentation] 造血器腫瘍幹細胞2014
Author(s)
赤司 浩一
Organizer
第3回日本血液学会東海地方会
Place of Presentation
名古屋
Year and Date
2014-04-26
Related Report
Invited
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