Analyses of transcriptional factor Fra-1 function with a focus on EMT

• Project/Area Number: 24659583
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Chiba University
• Principal Investigator: HOSHINO Isamu 千葉大学, 医学部附属病院, 医員 (10400904)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: Fra-1 / 食道扁平上皮癌 / HMGA1 / HMMR / EMT / 上皮間葉移行 / 足場非依存性増殖

Research Abstract

We have reported that Fra-1 was correlated with invasion and metastasis in esophageal squamous cell carcinoma(ESCC). Herein, we tried to clarify the mechanism of the Fra-1 expression in ESCC. We constructed Fra-1 knock down cells using siRNA in Fra-1 high expressed cells, TE10 and TE11. Fra-1 knock down induced reducing of cell proliferation, migration, and invasion potentials. Then, we explored the Fra-1 regulated genes using microarray and they were analyzed by BIOBASE Upstream Analysis. HMGA1 was a candidate which was regulated by Fra-1 and HMMR was detected as a gene which was regulated by HMGA1. Moreover, immunohistochemcal staining using clinical ESCC samples showed that HMMR was a significant prognostic factor in ESCC patients.

Research Products

• [Journal Article] The molecular role of Fra-1 and its prognostic significance in human esophageal squamous cell carcinoma. (2012)
  • Author(s): Usui A., Matsubara H., et al.
  • Journal Title: Cancer Volume: 118 Issue: 13 Pages: 3387-96
  • DOI: 10.1002/cncr.26652
  • Peer Reviewed
• [Presentation] 食道扁平上皮癌におけるFra-1制御遺伝子の網羅的検索 (2013)
  • Author(s): 豊住武司、星野敢、阿久津泰典, ほか
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2013-10-03
• [Presentation] 食道扁平上皮癌におけるFra-1制御遺伝子の網羅的検索 (2013)
  • Author(s): 豊住武司、星野敢、阿久津泰典, ほか
  • Organizer: 第34回癌免疫外科研究会
  • Place of Presentation: 岡山
  • Year and Date: 2013-05-17
• [Presentation] 食道扁平上皮癌におけるFra-1制御遺伝子の網羅的検索 (2013)
  • Author(s): 豊住武司、星野敢、阿久津泰典, ほか
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2013-04-12
• [Presentation] 食道扁平上皮癌におけるFra-1制御遺伝子の網羅的検索 (2013)
  • Author(s): 豊住 武司, 星野 敢, 阿久津 泰典, 羽成 直行, 森 幹人, 西森 孝典, 村上 健太郎, 赤沼 直毅, 遠藤 悟史, 磯崎 由佳, 竹下 修由, 丸山 哲朗, 小松 亜紀, 松原 久裕
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 食道扁平上皮癌におけるFra-1 制御遺伝子の網羅的検索 (2013)
  • Author(s): 豊住 武司, 星野 敢, 阿久津 泰典, 羽成 直行, 森 幹人, 西森 孝典, 村上 健太郎, 赤沼 直毅, 遠藤 悟史, 磯崎 由佳, 竹下 修由, 丸山 哲朗, 小松 亜紀, 松原 久裕
  • Organizer: 第34回癌免疫外科研究会
  • Place of Presentation: 岡山プラザホテル