| Project/Area Number |
24659583
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
HOSHINO Isamu 千葉大学, 医学部附属病院, 医員 (10400904)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | Fra-1 / 食道扁平上皮癌 / HMGA1 / HMMR / EMT / 上皮間葉移行 / 足場非依存性増殖 |
|---|
| Research Abstract |
We have reported that Fra-1 was correlated with invasion and metastasis in esophageal squamous cell carcinoma(ESCC). Herein, we tried to clarify the mechanism of the Fra-1 expression in ESCC. We constructed Fra-1 knock down cells using siRNA in Fra-1 high expressed cells, TE10 and TE11. Fra-1 knock down induced reducing of cell proliferation, migration, and invasion potentials. Then, we explored the Fra-1 regulated genes using microarray and they were analyzed by BIOBASE Upstream Analysis. HMGA1 was a candidate which was regulated by Fra-1 and HMMR was detected as a gene which was regulated by HMGA1. Moreover, immunohistochemcal staining using clinical ESCC samples showed that HMMR was a significant prognostic factor in ESCC patients.
|
