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Somatic cell to CMs by miRNA

Research Project

Project/Area Number 24659594
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

GOJO Satoshi  京都府立医科大学, 医学(系)研究科(研究院), 教授 (90316745)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
KeywordsncRNA / miRNA / 再生医療 / 心筋分化誘導 / 心筋分化
Outline of Final Research Achievements

Non-coding RNAs (ncRNAs) are involved in the regulation of most biological processes such as cancer, embryonic development, and cellular reprogramming. However, little is known about their roles in iPSC reprogramming. To elucidate the changing of non-coding RNAs during iPSC reprogramming, human fibroblasts TIG1 were infected with OCT4, SOX2, KLF4, and c-MYC (OSKM). Total RNAs from OSKM-TIG1 were analyzed for the ncRNAs expression.Expression of Y-RNA1 transiently increased during early stage (Day 0 to 9 after the transduction), and the localization was cellular cytoplasm. To evaluate the effect of the selected ncRNA, Y-RNA1, we assessed the efficacy of iPSCs reprogramming by the knockdown and over-expression experiments. The siRNA for Y-RNA1 was introduced to TIG1, where Y-RNA1 expression lessens to less than 10%, compared with conventional iPSC reprogramming. Our data demonstrate that Y-RNA1 is essential role for iPSC reprogramming, and works at the early stage of the process.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2015 2014 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (10 results)

  • [Journal Article] Pleiotropic functions of magnetic nanoparticles for ex vivo gene transfer2014

    • Author(s)
      Daisuke Kami, Tomoya Kitani, Tsunao Kishida, Osam Mazda, Masashi Toyoda, Asahi Tomitaka, Satoshi Ota, Ryuga Ishii, Yasushi Takemura, Masatoshi Watanabe, Akihiro Umezawa, and Satoshi Gojo.
    • Journal Title

      Nanomedicine

      Volume: In press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of a novel semi-quantitative analysis system for ultramicroscale samples by fluorescent capillary isoelectric focusing2014

    • Author(s)
      Daisuke Kami, Makoto Takahashi, Satoshi Gojo, Masashi Toyoda, Ryoko Aso, Ryuichi Sekizawa, Masatoshi Watanabe
    • Journal Title

      Biosensors & Bioelectronics

      Volume: 54 Pages: 656-660

    • DOI

      10.1016/j.bios.2013.11.047

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Placenta to Cartilage: Direct conversion of human placenta to chondrocytes with transformation by defined factors2012

    • Author(s)
      Kami D, Ishii R, Toyoda M, Makino H, Gojo S, Ishii T, Umezawa A
    • Journal Title

      Mol Biol Cell.

      Volume: 23(18) Issue: 18 Pages: 3511-21

    • DOI

      10.1091/mbc.e11-10-0869

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Amniotic membrane-derived mesenchymal stromal cell transplantation.2012

    • Author(s)
      Kimura M, Toyoda M, Gojo S3 Itakura Y, Kami D, Miyoshi S, Kyo S, Ono M, Umezawa A.
    • Journal Title

      J Stemcells & Regenerative Med

      Volume: 8 Pages: 171-180

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Non-coding Y-RNA1 is essential for iPS cell reprogramming2015

    • Author(s)
      Kami D., Kitani T., Toyoda M., Gojo S.
    • Organizer
      International Society for Stem Cell Research 13th Annual Meeting.
    • Place of Presentation
      Stockholm
    • Year and Date
      2015-06-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 磁性ナノ粒子を用いたex vivo gene therapyの開発2014

    • Author(s)
      上大介, 木谷友哉, 大多哲史, 冨高あさひ, 渡邉昌俊, 竹村泰司, 五條理志.
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] 磁性ナノ粒子を用いたsmall non-coding RNAの導入と細胞分離2014

    • Author(s)
      石田良, 河崎貴宣, 上大介, 五條理志
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] Efficient Transfection method
using the Magnetic nanoparticles and Episomal vector.2012

    • Author(s)
      Daisuke Kami, Masashi Toyoda, Atsushi Kishida, Osam Mazda, Akihiro Umezawa, Masatoshi Watanabe, Hiroaki Matsubara, Satoshi Gojo.
    • Organizer
      9th International Conference on the Scientific and Clinical Use of Magnetic Carriers.
    • Place of Presentation
      University of Minnesota
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒト右心耳由来心筋幹細胞の遺伝子導入による心筋分化誘導2012

    • Author(s)
      上大介, 豊田雅士, 板倉陽子, 松原弘明, 梅澤明弘, 五條理志
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] iPS細胞の早期・効率的取得を目指したキャピラリー等電点電気泳動法の開発2012

    • Author(s)
      上大介、高橋慎、豊田雅士、松原弘明、関澤隆一、渡邉昌俊、梅澤明弘、五條理志
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] CONSTRUCTION OF LARGE AMOUNTS CULTURE SYSTEM BY AN AUTOMATED CELL PROCESSING MACHINE FOR THE CELL TRANSPLANTATION2012

    • Author(s)
      Daisuke Kami, Masashi Toyoda, Mayu Yamazaki-Inoue, Naofumi Takehara, Takehiro Ogata, Hiroaki Matsubara, Akihiro Umezawa, Satoshi Gojo.
    • Organizer
      International Society for Stem Cell Research 10th Annual Meeting
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2012 Research-status Report
  • [Presentation] BASIC RESEARCH OF THE IMMUNO ASSAY BY THE CAPILLARY ISOELECTRIC FOCUSING FOR EFFICIENT ACQUISITION OF THE IPS CELL.2012

    • Author(s)
      Daisuke Kami, Makoto Takahashi, Masashi Toyoda, Ryuichi Sekizawa, Hiroaki Matsubara, Akihiro Umezawa, Satoshi Gojo.
    • Organizer
      International Society for Stem Cell Research 10th Annual Meeting
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2012 Research-status Report
  • [Presentation] DEVELOPMENT OF HIGH EFFICIENT GENE DELIVERY SYSTEM USING EPISOMAL VECTOR AND MAGNETIC NANOPARTICLES FOR PRODUCTION OF IPSCS.2012

    • Author(s)
      Daisuke Kami, Masashi Toyoda, Atsushi Kishida, Osam Mazda, Hiroaki Matsubara,Akihiro Umezawa, Satoshi Gojo.
    • Organizer
      International Society for Stem Cell Research 10th Annual Meeting
    • Place of Presentation
      Pacifico Yokohama
    • Related Report
      2012 Research-status Report
  • [Presentation] PLACENTA TO CARTILAGE: DIRECT CONVERSION OF HUMAN PLACENTA TO CHONDROCYTES WITH TRANSFORMATION BY DEFINED FACTORS.2012

    • Author(s)
      Daisuke Kami, Ryuga Ishii, Masashi Toyoda, Hatsune Makino, Satoshi Gojo, Akihiro Umezawa.
    • Organizer
      ISSCR-Roddenberry International Symposium on Cellular Reprogramming.
    • Place of Presentation
      GLADSTONE INSTITUTES
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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