Purification and culture of circulating tumor cells in gastrointestinal cancer patients

Research Project

Project/Area Number	24659607
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Digestive surgery
Research Institution	Osaka University
Principal Investigator	NISHIMURA Junichi 大阪大学, 医学(系)研究科(研究院), 助教 (20379209)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	遊離癌細胞 / miRNA / EpCAM-FITC / 大腸癌 / FACS
Research Abstract	We sorted circulating cells in blood of colorectal patients using Percoll treatment and FACS analysis. EpCAM+CD44+ cells were all CD45 positive. CD45-EpCAM+ cells were detected 10 cells of 4X105 cells. However, these cells were detected also in healthy volunteer, indicating that all these cells were not circulating cells originated from cancer cells. Next, we analyzed blood circulating miRNA in colorectal patients. miRNA expression of preoperative and postoperative blood samples were analyzed using microarray analysis. Four miRNAs were detected to be a colorectal cancer specific miRNA.