Elucidation of the MAML-focused network system of hypertrophic differentiation in chondrocytes.

• Project/Area Number: 24659667
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: ONO Takashi 東京大学, 医学部附属病院, 届出診療医 (00506248)
• Co-Investigator(Kenkyū-buntansha): INUI Hiroshi 東京大学, 医学部附属病院, 助教 (60583119)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 軟骨細胞 / 肥大分化 / 軟骨分化 / 軟骨細胞肥大分化 / 変形性関節症

Research Abstract

We checked the expression patterns of MAML family in mouse embryonic limb cartilage and found that MAML1 and MAML2 expressed in chondrocytes in hypertrophic zone. We further examined the expression patterns of MAML family by real-time RT-PCR during differentiation of cultured mouse chondrogenic cell line ATDC5 in the presence of insulin. The m-RNA levels of MAML1 and MAML2 were increased in their terminal differentiation stages. Overexression of MAML1 and MAML2 in ATDC5 cells and immature murine articular chondrocytes stimulated Mmp13 expression.