Analysis of molecular mechanisms in disk formation and homeostasis
Project/Area Number |
24659669
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Orthopaedic surgery
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
HIROSHI Asahara 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (70294460)
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | Mohawk / 椎間板 / 腱 / 靭帯 |
Outline of Final Research Achievements |
We have identified the transcription factor Mkx, that is expressed in tendon/ligament and plays a important role in tendon formation. The Mkx is also expressed in annulus fibrosus of intervertebral disc. The aged Mkx knockout mice showed degeneration of annulus fibrosus. Tendon markers, such as SCX and TNMD were reduced by MKX knockdown in human annulus fibroblasts whereas SOX9, a master chondrogenesis regulator, was upregulated. Moreover, overexpression of Mkx in C3H10T1/2 cells promoted the expressions of tendon markers, and chondrogenesis and osteogenesis were inhibited.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Targeted gene deletion of miRNAs in mice by TALEN system.2013
Author(s)
Takada S, Sato T, Ito Y, Yamashita S, Kato T, Kawasumi M, Kanai-Azuma M, Igarashi A, Kato T, Tamano M, Amahara H.
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Journal Title
PLoS One.
Volume: 8
Issue: 10
Pages: e76004-e76004
DOI
Related Report
Peer Reviewed
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[Journal Article] MAML1 enhances the transcriptional activity of Runx2 and plays a role in bone development.2013
Author(s)
Watanabe T, Oyama T, Asada M, Harada D, Ito Y, Inagawa M, Suzuki Y, Sugano S, Katsube K, Karsenty G, Komori T, Kitagawa M, Asahara H.
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Journal Title
PloS Genetics
Volume: 9(1)
NAID
Related Report
Peer Reviewed
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