Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Outline of Final Research Achievements |
We have identified the transcription factor Mkx, that is expressed in tendon/ligament and plays a important role in tendon formation. The Mkx is also expressed in annulus fibrosus of intervertebral disc. The aged Mkx knockout mice showed degeneration of annulus fibrosus. Tendon markers, such as SCX and TNMD were reduced by MKX knockdown in human annulus fibroblasts whereas SOX9, a master chondrogenesis regulator, was upregulated. Moreover, overexpression of Mkx in C3H10T1/2 cells promoted the expressions of tendon markers, and chondrogenesis and osteogenesis were inhibited.
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