Role of astrocyte metabolism in the constitution of long-term memory and the molecular pharmacological effect of anesthetics

• Project/Area Number: 24659703
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Aichi Medical University
• Principal Investigator: KINOSHITA Hiroyuki 愛知医科大学, 医学部, 教授 (70291490)
• Co-Investigator(Kenkyū-buntansha): NARITA Minoru 星薬科大学, 薬学部, 教授 (40318613) ; HATAKEYAMA Noboru 愛知医科大学, 医学部, 教授 (70251907) ; NAKAHATA Katsutoshi 和歌山県立医科大学, 医学部, 講師 (70332971)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 揮発性麻酔薬 / 脳 / 驚愕反応 / 薬理学

Outline of Final Research Achievements

Whether sevoflurane inhalation modulates the fear memory in young adult mice, have not been studied although neonatal exposure to this volatile anesthetic induces neurodegeneration resulting in deficits related to social behavior and/or learning disabilities. Since mice brain completes the growth during the first two weeks after birth, the current study was designed to examine whether sevoflurane exposure to four-weeks old young adult mice modifies the long-term fear memory learning ability, and if it is the case, whether F-actin constitution in the hippocampus relates to the consolidation of fear memory. Sevoflurane exposure to young adult mice accelerated the long-term horror learning ability with increased F-actin constitution in the hippocampus. These results suggest that sevoflurane amnesia may enhance fear memory consolidation resulting in psychological stress like posttraumatic stress disorder in young adults of humans.

Research Products

• [Presentation] 若年成体マウスへのセボフルラン暴露は恐怖記憶定着を促進する (2015)
  • Author(s): 中村絵美、橋本篤、ハロッド美佐子、椋田崇、藤原祥裕、木下浩之
  • Organizer: 日本麻酔科学会第62回学術集会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2015-05-28 – 2015-05-30
• [Presentation] Sevoflurane inhalation accelerates the long-term horror learning ability in young adult mice. (2014)
  • Author(s): Kinoshita H, Nakamura E, Feng G-G, MD, Li J, Jiang J, Sakakibara K, Fujiwara Y
  • Organizer: Annual Meeting of American Society of Anesthesiologist
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2014-10-11 – 2014-10-15