Identification and analysis of antigen for TRA98 which specifically recognizes germ cells

Research Project

Project/Area Number	24659713
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Urology
Research Institution	Osaka University
Principal Investigator	HIROSHI KIUCHI 大阪大学, 医学(系)研究科(研究院), 講師 (70403053)
Co-Investigator(Kenkyū-buntansha)	TANAKA Hiromitsu 長崎国際大学, 薬学部, 准教授 (10263310) TSUJIMURA Akira 順天堂大学, 医学部, 准教授 (40294053) MIYAGAWA Yasushi 大阪大学, 医学系研究科, 准教授 (70362704)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords	Germ Cell / Spermatogonia / Sperm / Spermatogenesis / Spermatocyte / Spermatid / PGC / ES / Germ　Cell / TRA98 / 生殖細胞
Outline of Final Research Achievements	TRA98, which specifically recognizes the germ cell nucleus, has been used as a marker of germ cells by many researchers at home and abroad. Nuclear antigen molecule that is recognized by the antibody (GENA) was unknown. We proceeded to identify the gene encoding the antigen recognized by the TRA98, and succeeded in cloning the gene. We examined knockout mice of the gene encoding the GENA antigen gene. In the female, fertility has been confirmed, but males showed infertility without spermatogonia. We found that the gene knockout mice are very important male infertility mice model.