Preeclampsia and placenta-specific microRNA

Research Project

Project/Area Number	24659737
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Obstetrics and gynecology
Research Institution	Showa University
Principal Investigator	SAITO HIROSHI 昭和大学, 医学部, 教授 (30119193)
Co-Investigator(Kenkyū-buntansha)	SEKIZAWA Akihiko 昭和大学, 医学部, 教授 (10245839) 四元淳子昭和大学, 医学部, 特別研究生 (30553648)
Co-Investigator(Renkei-kenkyūsha)	YOTSUMOTO Junko 昭和大学, 医学部, 特別研究生 (30553648)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	miRNA / 絨毛細胞 / 胎盤形成期 / 妊娠高血圧症候群 / 胎盤形成 / 妊娠初期
Research Abstract	To investigate whether there is a distinctive signature of microRNAs in the early stages of placentation by profiling the microRNA expression in chorionic villous samples at 6 and 11 weeks of gestation. Villous samples were collected from women at 6 and 11 weeks of gestation. Microarray approach was carried out to demonstrate differential miRNA expression between villous samples at 11 vs. 6 weeks of gestation. RT-PCR analysis was used to confirme the miRNA differential expression. Microarray identified 10 up-regulated miRNAs and 9 down-regulated miRNAs at 11 weeks compared with 6 weeks. Interestingly, 55% of those down-regulated miRNAs belong to the miRNA cluster coded in the 19q13.42. RT-PCR validated the lower expression of miR-520b, -520c and -520e and the higher expression of miR-30a-5p, -100-5p and -362-5p at 11 weeks. In conclusion, alterations on the expression of these miRNAs during placental development might have a role in later complications of pregnancy.