Analysis of N-glycans associated with diabetic retinopathy

• Project/Area Number: 24659754
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Hokkaido University
• Principal Investigator: ISHIDA Susumu 北海道大学, 医学(系)研究科(研究院), 教授 (10245558)
• Co-Investigator(Kenkyū-buntansha): NISHIMURA Shin-ichiro 北海道大学, 先端生命科学研究科, 教授 (00183898) ; AMANO Maho 北海道大学, 創成研究機構, 特任助教 (80365808)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 糖尿病網膜症 / 糖鎖 / グライコブロッティング

Research Abstract

Glycans are biopolymers bearing biological information and regarded as the third major class of cellular macromolecules, following nucleic acids and proteins. Glycosylation is an important process of post-translational modification that changes the functions of proteins. In this study, we analyzed the profile of N-glycans in human vitreous fluid obtained from patients with non-proliferative vitreoretinal diseases such as epiretinal membrane and macular hole (n=20). The concentration of N-glycans in vitreous samples was significantly lower compared with those in plasma samples. In both samples, predominant N-glycan was common and the structure is [(Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3(GlcNAc)2].

Research Products

• [Journal Article] A Comparison of N-glycan Profiles in Human Plasma and Vitreous Fluid
  • Author(s): Saori Inafuku, Kousuke Noda, Maho Amano, Tetsu Ohashi, Chikako Yoshizawa, Wataru Saito, Atsuhiro Kanda, Shin-ichiro Nishimura, and Susumu Ishida
  • Journal Title: Graefe's Archive for Clinical and Experimental Ophthalmology Volume: (in press)
  • Peer Reviewed
• [Presentation] Profile analysis of glycans in human vitreous (2013)
  • Author(s): Takashina S, Noda K, Amano M, Saito W, Nishimura S, Ishida S
  • Organizer: APVRS (8th Asia Pacific Vitreo-retina Society) Congress / 52nd Annual Meeting of Japanese Retina and Vitreous Society
  • Place of Presentation: Nagoya, Japan
• [Presentation] Profile analysis of glycans in human vitreous (2013)
  • Author(s): Takashina S, Noda K, Amano M, Saito W, Nishimura S, Ishida S.
  • Organizer: The 8th Asia Pacific Vitreo-retina Society Congress/52nd Annual Meeting of Japanese Retina and Vitreous Society
  • Place of Presentation: Nagoya Congress Center (Nagoya)