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Regulation of bone resorption by membrane reparing molecules: Live imaging of the specific fusion among osteoclast precursors

Research Project

Project/Area Number 24659816
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionKyushu University

Principal Investigator

KUKITA TOSHIO  九州大学, 歯学研究科(研究院), 教授 (70150464)

Co-Investigator(Kenkyū-buntansha) KUKITA Akiko  佐賀大学, 医学部, 准教授 (30153266)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords破骨細胞 / 分化 / 膜融合 / 膜修復 / MG53 / 骨吸収制御 / 膜修復分子 / 細胞融合 / 膜ナノチューブ / M53 / DC-STAMP / アポトーシス / 細胞分化
Research Abstract

When the muscle cell membrane was subjected to the damage, the membrane was immediately repaired by the action of "membrane repairing molecules". Osteoclasts are formed by fusion of mononuclear osteoclast precursors, and this process is postulated to be guarranteed by the action of "membrane reparing mechanisms". The purpose of this research is to identify MG53 or its analogue and involvement of membrane repairing mechanism" during the process of osteoclast differentiation. Unstimulated osteoclast precursors expressed MG53. However, its expression was dramatically suppressed after being stimulated to form osteoclasts. Our data suggested that the "membrane repairing molecule" could act as the negative regulator for osteoclast differentiation.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (27 results)

All 2014 2013 2012 Other

All Journal Article (21 results) (of which Peer Reviewed: 19 results,  Open Access: 1 results) Presentation (6 results)

  • [Journal Article] Mesenchymal stem cell markedly suppress inflammatory bone destruction in rats with adjuvant-induced aerthritis2014

    • Author(s)
      Takano T., Li Y-J., Kukita A., Yamaza T., Ayukawa Y., Moriyama K., Uehara N., Nomiyama H., Koyano K., Kukita T
    • Journal Title

      Lab Invest

      Volume: 94 Pages: 286-296

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mesenchymal stem cells markedly suppress inflammatory bone destruction in rats with adjuvant-induced arthritis2014

    • Author(s)
      Takano, T. Li, Y. J. Kukita, A. Yamaza, T. Ayukawa, Y. Moriyama, K. Uehara, N. Nomiyama, H. Koyano, K. and Kukita, T.
    • Journal Title

      Laboratory Investigation

      Volume: 94 Issue: 3 Pages: 286-296

    • DOI

      10.1038/labinvest.2013.152

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multifunctional properties of RANKL/RANK in cell differentiation, proliferation and metastasis2013

    • Author(s)
      Kukita A, Kukita T
    • Journal Title

      Future Oncology

      Volume: 9 Pages: 1609-1622

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immune hterapeutic potential of stem cells from human supernumerary teeth2013

    • Author(s)
      Makino Y., Yamaza H., Akiyama K., Ma L., Hoshino Y., Terada Y., Kukita T., Shi S., Yamaza T
    • Journal Title

      J Dent Res

      Volume: 92 Pages: 609-615

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tunneling nanotube formation is essential for the regulation of osteoclastogenesis2013

    • Author(s)
      Takahashi A., Kukita A., Li Y-J., Zhang JQ., Nomiyama H., Yamaza T., Ayukawa Y., Koyano K., Kukita T
    • Journal Title

      J Cell Biochem

      Volume: 114 Pages: 1238-1247

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adenosine blocks aminopterin-induced suppression of osteoclast differentiation2013

    • Author(s)
      Teramachi J., Kukita A., Wada H., Kukita, T
    • Journal Title

      J Bone Min Metab

      Volume: 31 Pages: 64-67

    • NAID

      10031168838

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multifunctional properties of RANKL/RANK in cell differentiation, proliferation and metastasis.2013

    • Author(s)
      Kukita A, Kukita T.
    • Journal Title

      Future Oncol.

      Volume: 9 Issue: 11 Pages: 1609-1622

    • DOI

      10.2217/fon.13.115

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tunneling nanotube formation is essential for the regulation of osteoclastogenesis2013

    • Author(s)
      Takahashi, A. Kukita, A. Li, Y. J. Zhang, J. Q. Nomiyama, H. Yamaza, T. Ayukawa, Y. Koyano, K. and Kukita, T.
    • Journal Title

      Journal of Cellular Biochemistry

      Volume: 114 Issue: 6 Pages: 1238-1247

    • DOI

      10.1002/jcb.24433

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adenosine blocks aminopterin-induced suppression of osteoclast differentiation.2013

    • Author(s)
      Teramachi J., Kukita A., Qu P-F., Wada N., Li Y-J., Kukita T.
    • Journal Title

      J Bone Miner Metab.

      Volume: 31 Issue: 1 Pages: 64-70

    • DOI

      10.1007/s00774-012-0388-7

    • NAID

      10031168838

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tunneling nanotube formation is essential for the regulation of osteoclastogenesis.2013

    • Author(s)
      Takahashi A., Kukita A., Li Y-J., Zhang JQ., Nomiyama H., Yamaza T., Ayukawa Y., Koyano K., Kukita T.
    • Journal Title

      J.Cell.Biochem.

      Volume: (in press) Pages: 1-11

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Adenosine blocks aminopterin-induced suppression of osteoclast differentiation.2013

    • Author(s)
      Teramachi J., Kukita A., Wada H., Kukita, T.
    • Journal Title

      J Bone Min Metab.

      Volume: 31 Pages: 64-70

    • NAID

      10031168838

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cryoprerserved dental pulp tussues of exfoliated deciduous teeth is a feasible stem cell resourced for regenerative medicine2012

    • Author(s)
      Ma L., Makino Y., Yamaza H.,Akiyama K., Hoshino Y., Somg G., Kukita T., Nonaka K., Shi S., Yamaza T
    • Journal Title

      PLoS One

      Volume: 7 Pages: 51777-51777

    • Related Report
      2013 Final Research Report
  • [Journal Article] Nordihydroguaiaretic acid suppresses osteoclastogenesis by inhibiting NFATc1 and suppresses bone destruction accompanying adjuvant arthritis in rats2012

    • Author(s)
      Li Y-J., Kukita A., Takano T., Qu P., Sanematsu K., Ninomiya Y., Kukita T
    • Journal Title

      Lab.Invest

      Volume: 92 Pages: 1777-1787

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of and identification of subpopulations of mononuclear Preosteoclasts induced by TNFαin combination with TGFα2012

    • Author(s)
      Matsubara R., Kukita T., Ichigi Y., Takigawa I., Qu P., Nonaka K., Kukita A
    • Journal Title

      PLosOne

      Volume: 7(in press) Pages: 47930-47930

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Infection of RANKL-primed RAW-D macrophages with Porphyromonas gingivalis promotes osteoclastogenesis in a TNFα-independent manner2012

    • Author(s)
      Kukita A., Ichigi Y., Takigawa I., Watanabe T., Kukita T., Miyamoto H
    • Journal Title

      PLosOne

      Volume: 7 Pages: 38500-38500

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stage-specificfunction of leukemia/ lymphoma-related factor (LRF) in the transcriptional control of osteoclast development2012

    • Author(s)
      Tsuji-Takeuchi K., Negishi- Koga T., Sumiya E. Kukita A., Kato S., Maeda T., Pandolfi PP. Moriyama K., Takayanagai H
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 109 Pages: 2561-2566

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 破骨細胞分化と機能を制御する転写因子の役割2012

    • Author(s)
      久木田明子、菖蒲池健夫、久木田敏夫
    • Journal Title

      化学と生物

      Volume: 50 Pages: 488-497

    • Related Report
      2013 Final Research Report
  • [Journal Article] Nordihydroguaiaretic acid suppresses osteoclastogenesis by inhibiting NFATc1 and suppresses bone destruction accompanying adjuvant arthritis in rats.2012

    • Author(s)
      Li Y-J., Kukita A., Takano T., Qu P., Sanematsu K., Ninomiya Y., Kukita T.
    • Journal Title

      Lab.Invest.

      Volume: 92(12) Pages: 1777-1787

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of and identification of subpopulations of mononuclear Preosteoclasts induced by TNFα in combination with TGFβ.2012

    • Author(s)
      Matsubara R., Kukita T., Ichigi Y., Takigawa I., Qu P., Nonaka K., Kukita A.
    • Journal Title

      PLosOne

      Volume: 7(10)

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Induction of RANKL-primed RAW-D macrophages with Porphyromonas gingivalis promotes osteoclastogenesis in a TNF-independent manner.2012

    • Author(s)
      Kukita A., Ichigi Y., Takigawa I., Watanabe T., Kukita T., Miyamoto H.
    • Journal Title

      PLosOne

      Volume: 7(6)

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of osteoclastogenesis through Tim-3 : A possible involvement of Tim-3/galectin-9 system in the modulation of inflammatory bone destruction

    • Author(s)
      Moriyama K., Kukita A., Li-Y-J., Uehara N., Zhang J-Q., Takahashi I. and Kukita T
    • Journal Title

      Lab Invest

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 炎症抑制ガレクチン9の膜表面受容体Tim3を介した破骨細胞形成制御2013

    • Author(s)
      森山加奈子、久木田明子、張旌旗、上原範久、高橋一郎、久木田敏夫
    • Organizer
      第55回歯科基礎医学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] 膜ナノチューブを介する破骨前駆細胞間融合の走査電顕的解析張旌旗2013

    • Author(s)
      高橋良、久木田明子、成松加奈子、上原範久、山座孝義、城戸瑞穂、久木田敏夫
    • Organizer
      第55回歯科基礎医学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] A Possible Regulation of Osteoclast Differentiation by Galectin-92013

    • Author(s)
      Kanako Moriyama, Akiko Kukita, Ichiro Takahashi, Toshio Kukita
    • Organizer
      2^<nd> Joint Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] WT1アンチセンスRNAの成熟破骨細胞に於ける高発現と分化制御機能2012

    • Author(s)
      李銀姫、久木田明子、久木田敏夫
    • Organizer
      第117回日本解剖学会
    • Place of Presentation
      山梨
    • Related Report
      2013 Final Research Report
  • [Presentation] ノルジヒドログアイアレチン酸(NDGA)によるヒト肺癌骨転移制御2012

    • Author(s)
      李銀姫、渡辺敏之、高橋良、久木田明子、瀧口総一、井口東郎、久木田敏夫
    • Organizer
      第30回日本骨代謝学会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] 伝統的秘薬NDGAによる炎症性骨破壊制御2012

    • Author(s)
      李銀姫、久木田明子、渡辺敏之、屈鵬飛、久木田敏夫
    • Organizer
      解剖学会九州地方会
    • Place of Presentation
      久留米
    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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