| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Human TMJ cells were isolated and cultured from surgically resected mandibular condyles, and were transfected with either NF-kB decoy, which are constituted of synthetic double stranded oligodeoxynucleotide (17bp) including consensus sequence of NF-kB binding site (7bp), or mutant type decoy using the HVJ (hemagglutinating virus of Japan)-liposome method. Transfected and non-transfected cells were stimulated by TNF-alpha (-100ng/ml) and examined for uPA and MMP-1 mRNAs and protein expression by a Northern blot analysis and ELISA. The expression of uPA and MMP-1 mRNAs of TMJ cells was enhanced by TNF-alpha stimulation in a dose dependent manner. While uPA and MMP-1 mRNAs and protein expression by cultured cells were not affected by the treatment with either HVJ-liposome containing mutant type decoy or empty one, transfection of wild type decoy decreased uPA and MMP-1 mRNAs expression to 30% and proteins to about 40% as compared with non-transfected and TNF-alpha stimulated cells.
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