Development of biopolymer compound to elucidate the effect of glucan on innate immune system
| Project/Area Number |
24659841
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pathobiological dentistry/Dental radiology
|
|---|
| Research Institution | Kyushu Dental College |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ARIYOSHI Wataru 九州歯科大学, 歯学部, 准教授 (40405551)
沖永 敏則 九州歯科大学, 歯学部, 助教 (60582773)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
OKINAGA Toshinori 九州歯科大学, 歯学部, 助教 (60582773)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | マクロファージ / β1-3グルカン / dectin-1 / インフラマソーム |
|---|
| Research Abstract |
Several immune system cell-surface receptors are reported to be associated with osteoclastogenesis. Dectin-1, a lectin receptor for beta-glucan, is found predominantly on cells of the myeloid lineage. In the present study, we examined the effect of dectin-1 agonist, curdlan, on osteoclastogenesis. In dectin-1 over-expressing RAW 264.7 cells (d-RAW), curdlan suppressed receptor activator of nuclear factor-kappaB (NF-kappaB) ligand (RANKL)-induced osteoclast differentiation, bone resorption and actin-ring formation. Furthermore, curdlan inhibited RANKL-induced nuclear factor of activated T-cell cytoplasmic 1 (NFATc1) expression.
In conclusion, our results demonstrate that curdlan potentially inhibits osteoclast differentiation, especially NFATc1 expression. This suggests activation of curdlan-dectin-1 interaction critically regulates the genes required for osteoclastogenesis.
|
Report
(3 results)
Research Products
(95 results)
-
[Journal Article] Factors associated with dry mouth in dependent Japanese elderly2014
Author(s)
Kakudate N, Muramatsu T, Endoh M, Satomura K, Koseki T, Sato Y, Ito K, Ogasawara T, Nakamura S, Kishimoto E, Kashiwazaki H, Yamashita Y, Uchiyama K, Nishihara T, Kiyohara Y, Kakinoki Y
-
Journal Title
Gerodontology
Volume: 31(1)
Issue: 1
Pages: 11-8
DOI
Related Report
Peer Reviewed
-
[Journal Article] Inflammatory bone loss in experimental periodontitis induced by Aggregatibacter actinomycetemcomitans in IL-1Ra knockout mice.2014
Author(s)
Izawa A, Ishihara Y, Mizutani H, Kobayashi S, Goto H, Okabe E, Takeda H, Ozawa Y, Kamiya Y, Sugita Y, Kubo K, Kamei H, Kikuchi T, Mitani A, Hayashi J, Nishihara T, Maeda H, Noguchi T.
-
Journal Title
Infect Immun
Volume: 82
Issue: 5
Pages: 1904-1913
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Lipopolysaccharide of Aggregatibacter actinomycetemcomitans up-regulates inflammatory cytokines, prostaglandin E2 synthesis and osteoclast formation in interleukin-1 receptor antagonist-deficient mice2013
Author(s)
Mizutani H, Ishihara Y, Izawa A, Fujihara Y, Kobayashi S, Gotou H, Okabe E, Takeda H, Ozawa Y, Kamiya Y, Kamei H, Kikuchi T, Yamamoto G, Mitani A, Nishihara T, Noguchi T.
-
Journal Title
J Periodont Res
Volume: 48
Issue: 6
Pages: 748-756
DOI
Related Report
Peer Reviewed
-
[Journal Article] Mechanisms of G1 cell cycle arrest and apoptosis in myeloma cells induced by hybrid-compound histone deacetylase inhibitor2013
Author(s)
Fujii, S., Okinaga, T., Ariyoshi, W., Takahashi, O., Iwanaga, K., Nishino, N., Tominaga, K., Nishihara, T
-
Journal Title
Biochem Biophys Res Commun
Volume: 434
Issue: 3
Pages: 413-20
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Classification of periodontal disease patients by FT-IR.2013
Author(s)
SATOSHI FUJII, KEISUKE FUKUDA、SHINOBU SATO, TOSHINORI OKINAGA, WATARU ARIYOSHI, KEISUKE NAKASHIMA, TATSUJI NISHIHARA, SHIGEORI TAKENAKA
Organizer
Asia-Pacific Conference in Fukuoka 2013- International Symposium on Oral Education and Research in Kitakyushu
Place of Presentation
Kitakyushu, Japan
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
