Development of the iPS cell-based diagnostic technology for dental treatments
| Project/Area Number |
24659858
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
EGUSA Hiroshi 大阪大学, 歯学研究科(研究院), 助教 (30379078)
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| Co-Investigator(Kenkyū-buntansha) |
YATANI Hirofumi 大阪大学, 大学院歯学研究科, 教授 (80174530)
NAKANO Tamaki 大阪大学, 大学院歯学研究科, 助教 (40379079)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | iPS細胞 / 骨芽細胞 / 個体差 |
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| Research Abstract |
Induced pluripotent stem (iPS) cells, which can be generated individual patients, have potential to represent the patient-specific cell responses in vitro. In this study, we attempted to generate integration-free iPS cells from patients' gingiva using episomal plasmid vectors, and successfully generated iPS cell clones. We next evaluated optimal osteogenic induction of iPS cells using mouse gingiva-derived iPS cells, demonstrating that iPS cells can be guided to differentiate into mature osteoblasts with hydroxyapatite formation. These results suggest that the firm method to guide integration-free iPS cells to differentiate into osteoblasts may lead to a new technology for detecting individual differences in the clinical outcome of bone regeneration.
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Report
(3 results)
Research Products
(42 results)
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[Journal Article] Comparative Analysis of Mouse Induced Pluripotent Stem Cells and Mesenchymal Stem Cells during Osteogenic Differentiation in vitro.2014
Author(s)
Egusa, H., Kayashima, H., Miura, J., Uraguchi, S., Wang, F., Okawa, H., Sasaki, J.I., Saeki, M., Matsumoto, T., and Yatani, H.
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Journal Title
Stem cells and development.
Volume: 23
Issue: 18
Pages: 2156-2169
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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