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Development of the iPS cell-based diagnostic technology for dental treatments

Research Project

Project/Area Number 24659858
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Prosthetic dentistry
Research InstitutionOsaka University

Principal Investigator

EGUSA Hiroshi  大阪大学, 歯学研究科(研究院), 助教 (30379078)

Co-Investigator(Kenkyū-buntansha) YATANI Hirofumi  大阪大学, 大学院歯学研究科, 教授 (80174530)
NAKANO Tamaki  大阪大学, 大学院歯学研究科, 助教 (40379079)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsiPS細胞 / 骨芽細胞 / 個体差
Research Abstract

Induced pluripotent stem (iPS) cells, which can be generated individual patients, have potential to represent the patient-specific cell responses in vitro. In this study, we attempted to generate integration-free iPS cells from patients' gingiva using episomal plasmid vectors, and successfully generated iPS cell clones. We next evaluated optimal osteogenic induction of iPS cells using mouse gingiva-derived iPS cells, demonstrating that iPS cells can be guided to differentiate into mature osteoblasts with hydroxyapatite formation. These results suggest that the firm method to guide integration-free iPS cells to differentiate into osteoblasts may lead to a new technology for detecting individual differences in the clinical outcome of bone regeneration.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (42 results)

All 2014 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (32 results) (of which Invited: 16 results)

  • [Journal Article] Comparative Analysis of Mouse Induced Pluripotent Stem Cells and Mesenchymal Stem Cells during Osteogenic Differentiation in vitro.2014

    • Author(s)
      Egusa, H., Kayashima, H., Miura, J., Uraguchi, S., Wang, F., Okawa, H., Sasaki, J.I., Saeki, M., Matsumoto, T., and Yatani, H.
    • Journal Title

      Stem cells and development.

      Volume: 23 Issue: 18 Pages: 2156-2169

    • DOI

      10.1089/scd.2013.0344

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 歯肉を用いた医療応用に安全なiPS細胞の開発―新たな再生歯科医療技術の創成に向けて―2014

    • Author(s)
      江草宏,于冠男,鎌野優弥,大川博子,山本治毅,矢谷博文
    • Journal Title

      日歯医学会誌

      Volume: 33 Pages: 54-58

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 歯肉を用いた医療応用に安全なiPS 細胞の開発 ―新たな再生歯科医療技術の創成に向けて―2014

    • Author(s)
      江草 宏,于 冠男,鎌野優弥,大川博子,山本治毅,矢谷博文
    • Journal Title

      日歯医学会誌

      Volume: 33 Pages: 54-58

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frontier dental research on iPS cells2013

    • Author(s)
      Arakaki M, Egusa H, Otsu K, Saitoh I, Miura T, Harada H
    • Journal Title

      Journal of Oral Biosciences

      Volume: 55:4 Issue: 4 Pages: 159-164

    • DOI

      10.1016/j.job.2013.08.002

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] iPS細胞の骨生物学への応用と歯科臨床への展望2013

    • Author(s)
      江草 宏
    • Journal Title

      医学生物学電子顕微鏡技術学会誌

      Volume: 27 Pages: 67-69

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Stem cells in dentistry – Part I: Stem cell sources2012

    • Author(s)
      Egusa H, Sonoyama W, Nishimura M, Atsuta I, Akiyama K
    • Journal Title

      Journal of Prosthodontic Research

      Volume: 56 Issue: 3 Pages: 151-165

    • DOI

      10.1016/j.jpor.2012.06.001

    • NAID

      130004546652

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] (対談)iPS細胞と遺伝子診断で進化する再生医療~最先端のPersonalized Dentistry2012

    • Author(s)
      江草宏,西村一郎
    • Journal Title

      GC Circle

      Volume: 142 Pages: 4-11

    • Related Report
      2013 Final Research Report
  • [Journal Article] Stem cells in dentistry – Part II: Clinical applications2012

    • Author(s)
      Egusa H, Sonoyama W, Nishimura M, Atsuta I, Akiyama K
    • Journal Title

      Journal of Prosthodontic Research

      Volume: 56 Issue: 4 Pages: 229-248

    • DOI

      10.1016/j.jpor.2012.10.001

    • NAID

      130004546661

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 対談:iPS細胞と遺伝子診断で進化する再生医療~最先端のPersonalized Dentistry2012

    • Author(s)
      江草 宏,西村一郎
    • Journal Title

      GC Circle

      Volume: 142 Pages: 4-11

    • Related Report
      2012 Research-status Report
  • [Journal Article] iPS細胞の骨生物学への応用と歯科臨床への展望

    • Author(s)
      江草宏
    • Journal Title

      医学生物学電子顕微鏡技術学会誌

      Volume: 27(2)(印刷中)

    • Related Report
      2013 Final Research Report
  • [Presentation] Application of iPS Cells in bone biology2014

    • Author(s)
      江草宏
    • Organizer
      Kyudai Oral Bioscience 2014国際シンポジウム"Oral Stem Cells"
    • Place of Presentation
      福岡市
    • Year and Date
      2014-02-28
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS Cells in Regenerative Dental Medicine2014

    • Author(s)
      江草宏
    • Organizer
      The 5th International Symposium for Interface Oral Health Science国際シンポジウム"Cell Manipulation and Tissue Regeneration"
    • Place of Presentation
      仙台市
    • Year and Date
      2014-01-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Stem cells in regenerative dental medicine2013

    • Author(s)
      江草宏
    • Organizer
      2013 Thailand International Conference on Oral Biology国際シンポジウム"Stem Cells and Tissue Engineering"
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2013-11-28
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS細胞が可能にする歯科医療とは2013

    • Author(s)
      江草宏
    • Organizer
      平成25年度日本補綴歯科学会関西支部学術大会生涯学習公開セミナー「歯科におけるバイオサイエンスの基礎と応用」
    • Place of Presentation
      大阪市
    • Year and Date
      2013-11-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 歯肉から作るiPS細胞が可能にする歯科医療とは2013

    • Author(s)
      江草宏
    • Organizer
      第25回日本小児口腔外科学会学術大会シンポジウム「小児口腔外科と再生医療」
    • Place of Presentation
      東京都
    • Year and Date
      2013-11-02
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS細胞を用いたスキャフォールドフリー骨組織再生2013

    • Author(s)
      江草宏
    • Organizer
      第55回歯科基礎医学会学術大会シンポジウム「Biodental Engineering~バイオ研究と材料科学の融合~」
    • Place of Presentation
      岡山市
    • Year and Date
      2013-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS細胞の骨生物学への応用と歯科臨床への展望2013

    • Author(s)
      江草宏
    • Organizer
      第29回医学生物学電子顕微鏡技術学会特別講演
    • Place of Presentation
      横須賀市
    • Year and Date
      2013-06-09
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS細胞の骨形成能と歯科臨床への展望2013

    • Author(s)
      江草宏
    • Organizer
      第67回日本口腔科学会学術集会シンポジウム「歯周組織における再生医療」
    • Place of Presentation
      宇都宮市
    • Year and Date
      2013-05-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of gingiva -derived iPS cell technologies in regenerative dental medicine2013

    • Author(s)
      江草宏
    • Organizer
      International Symposium on Human Resource Development towards Global Initiative国際シンポジウム"Regenerative Dental Medicine"
    • Place of Presentation
      Cha-am, Thailand
    • Year and Date
      2013-02-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of gingiva -derived iPS cell technologies in dental research2012

    • Author(s)
      江草宏
    • Organizer
      第60回国際歯科研究学会日本部会(JADR)学術大会国際シンポジウム"Clinically Relevant Basic Research in the Prosthodontic Field"
    • Place of Presentation
      新潟市
    • Year and Date
      2012-12-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of gingiva -derived iPS cells in bone biology2012

    • Author(s)
      江草宏
    • Organizer
      The 2nd International Anatomical Sciences and Cell Biology Conference国際シンポジウム"Stem Cells and Tissue Engineering"
    • Place of Presentation
      Chiang Mai, Thailand
    • Year and Date
      2012-12-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 歯肉から作る万能細胞(iPS細胞)が可能にする歯科医療とは2012

    • Author(s)
      江草宏
    • Organizer
      神奈川歯科大学学会研究談話会講演
    • Place of Presentation
      横須賀市
    • Year and Date
      2012-11-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 歯肉由来iPS細胞の歯科医学への応用2012

    • Author(s)
      江草宏
    • Organizer
      第54回歯科基礎医学会学術大会シンポジウム「口腔領域におけるiPS研究の現状と展望」
    • Place of Presentation
      郡山市
    • Year and Date
      2012-09-14
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS細胞の骨生物学への応用2012

    • Author(s)
      江草宏
    • Organizer
      硬組織再生生物学会第21回学術大会特別講演
    • Place of Presentation
      名古屋市
    • Year and Date
      2012-08-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Gingiva-derived iPS cells : A promising tool for dental applications2012

    • Author(s)
      江草宏
    • Organizer
      大阪大学歯学部・大学院歯学研究科「口の難病から挑むライフ・イノベーション」プロジェクト日本・韓国国際シンポジウム"Development and Regeneration of Oro-Facial Structures"
    • Place of Presentation
      吹田市
    • Year and Date
      2012-07-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Gingiva-derived iPS cells : A promising tool for dental applications2012

    • Author(s)
      江草宏
    • Organizer
      The 90th IADR General Session国際シンポジウム"Stem Cell Niches and Pluripotent Stem Cells for Craniofacial Regeneration"
    • Place of Presentation
      Iguacu Falls, Brazil
    • Year and Date
      2012-06-20
    • Related Report
      2013 Final Research Report
  • [Presentation] iPS細胞の骨形成能と歯科臨床への展望

    • Author(s)
      江草 宏
    • Organizer
      第67回 日本口腔科学会学術集会 シンポジウム「歯周組織における再生医療」
    • Place of Presentation
      宇都宮市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] iPS細胞の骨生物学への応用と歯科臨床への展望

    • Author(s)
      江草 宏
    • Organizer
      第29回 医学生物学電子顕微鏡技術学会 特別講演
    • Place of Presentation
      横須賀市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] iPS細胞を用いたスキャフォールドフリー骨組織再生

    • Author(s)
      江草 宏
    • Organizer
      第55回 歯科基礎医学会学術大会 シンポジウム「Biodental Engineering ~バイオ研究と材料科学の融合~」
    • Place of Presentation
      岡山市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 歯肉から作るiPS細胞が可能にする歯科医療とは

    • Author(s)
      江草 宏
    • Organizer
      第25回 日本小児口腔外科学会学術大会 シンポジウム「小児口腔外科と再生医療」
    • Place of Presentation
      東京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 歯肉から作るiPS細胞が可能にする歯科医療とは

    • Author(s)
      江草 宏
    • Organizer
      日本補綴歯科学会関西支部学術大会 生涯学習公開セミナー「歯科におけるバイオサイエンスの基礎と応用」
    • Place of Presentation
      大阪市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Stem cells in regenerative dental medicine

    • Author(s)
      江草 宏
    • Organizer
      2013 Thailand International Conference on Oral Biology 国際シンポジウム“Stem Cells and Tissue Engineering”
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] iPS Cells in Regenerative Dental Medicine

    • Author(s)
      江草 宏
    • Organizer
      The 5th International Symposium for Interface Oral Health Science 国際シンポジウム“Cell Manipulation and Tissue Regeneration”
    • Place of Presentation
      仙台市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Application of iPS Cells in bone biology

    • Author(s)
      江草 宏
    • Organizer
      Kyudai Oral Bioscience 2014 国際シンポジウム “Oral Stem Cells”
    • Place of Presentation
      福岡市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Gingiva-derived iPS cells: A promising tool for dental applications

    • Author(s)
      江草 宏
    • Organizer
      The 90th IADR General Session 国際シンポジウム
    • Place of Presentation
      Iguacu Falls, Brazil
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Gingiva-derived iPS cells: A promising tool for dental applications

    • Author(s)
      江草 宏
    • Organizer
      大阪大学歯学部・大学院歯学研究科 日本・韓国国際シンポジウム
    • Place of Presentation
      大阪府吹田市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] iPS細胞の骨生物学への応用

    • Author(s)
      江草 宏
    • Organizer
      硬組織再生生物学会 第21回学術大会 特別講演
    • Place of Presentation
      名古屋市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 歯肉由来iPS細胞の歯科医学への応用

    • Author(s)
      江草 宏
    • Organizer
      第54回 歯科基礎医学会学術大会 シンポジウム
    • Place of Presentation
      郡山市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 歯肉から作る万能細胞(iPS細胞)が可能にする歯科医療とは

    • Author(s)
      江草 宏
    • Organizer
      神奈川歯科大学学会 研究談話会講演
    • Place of Presentation
      横須賀市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Application of gingiva-derived iPS cells in bone biology

    • Author(s)
      江草 宏
    • Organizer
      The 2nd International Anatomical Sciences and Cell Biology Conference 国際シンポジウム
    • Place of Presentation
      Chiang Mai, Thailand
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Application of gingiva-derived iPS cell technologies in dental research

    • Author(s)
      江草 宏
    • Organizer
      第60回 国際歯科研究学会 日本部会(JADR)学術大会 国際シンポジウム
    • Place of Presentation
      新潟市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Application of gingiva-derived iPS cell technologies in regenerative dental medicine

    • Author(s)
      江草 宏
    • Organizer
      International Symposium on Human Resource Development towards Global Initiative 国際シンポジウム
    • Place of Presentation
      Cha-am, Thailand
    • Related Report
      2012 Research-status Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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