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Circulating tumor-derived mutant mitochondrial DNA: a predictive biomarker of clinical prognoses in human squamous cell carcinoma

Research Project

Project/Area Number 24659885
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionChiba University

Principal Investigator

TANZAWA HIDEKI  千葉大学, 医学(系)研究科(研究院), 教授 (50236775)

Co-Investigator(Kenkyū-buntansha) KOIKE Hirofumi  千葉大学, 医学部附属病院, 助教 (10595995)
KOUZU Yukinao  千葉大学, 大学院医学研究院, 助教 (70400942)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsmtDNA / 口腔癌 / qPCR-HRMA / 予後判定 / mitochondrial DNA(mtDNA) / qPCR-HRMA法 / 癌再発の早期診断法
Research Abstract

In a variety of malignant tumors, it is difficult to detect the circulating tumor-derived molecular targets. But ,in murine xenografting human oral cancer cells, human mtDNAs could be quantitatively detected from organs and blood samples. In the present study, we used this mtDNA for patients with oral cancer, and analyzed all mitochondrial loop domains. As a result, we found that the oral cancer derived cell lines had mutation at 5 regions in mtDNA. mtDNA from normal and tumorous tissues and serum mtDNA obtained preoperatively and 4 weeks postoperatively from 61 subjects was examined at these five different regios. All patients with a poor prognosis (recurrence and/or distant metastasis cases, r/m+) had significantly (P<0.05) higher amounts of mutant mtDNAs in the tumoral tissues. These results suggested that this approach may have useful diagnosis for predictor of the prognosis.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Circulating tumor-derived mutant mitochondrial DNA : a predictive biomarker of clinical prognosis in human squamous cell carcinoma2012

    • Author(s)
      Uzawa K, Baba T, Uchida F, Yamatoji M, Kasamatsu A, Sakamoto Y, Ogawara K, Shiiba M, Bukawa H, Tanzawa H
    • Journal Title

      Oncotarget

      Volume: 3(7) Pages: 670-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Circulating tumor-derived mutant mitochondrial DNA in oral cancer2013

    • Author(s)
      Baba T, Uzawa K, Kasamatsu A, Sakamoto Y, Ogawara K, Shiiba M, Tanzawa H
    • Organizer
      The IADR 91^<st> General Session & Exhibition
    • Place of Presentation
      Seattle, Washington, USA
    • Related Report
      2013 Final Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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