Identification of glossodynia-associated saliva microRNA with the use of next generation sequencing

• Project/Area Number: 24659897
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Ehime University
• Principal Investigator: NAKASHIRO Koichi 愛媛大学, 医学(系)研究科(研究院), 准教授 (90314880)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 舌痛症 / 唾液 / microRNA / 次世代シーケンサー

Research Abstract

In this study, we attemptedIn this study, we attempted to identify a specific microRNA (miRNA) in saliva derived from patients with glossodynia. By next generation sequencing, no specific miRNA was detected in saliva from glossodynia patients. In microarray analysis, we found 6 increased miRNAs in saliva from glossodynia patients. These miRNAs may be useful for diagnosis of glossodynia.

Research Products

• [Journal Article] Immuno- chemoradiotherapy for patients with oral squamous cell carcinoma : augmentation of OK-432-induced helper T cell 1 response by 5-FU and X-ray irradiation (2013)
  • Author(s): Tano T, Okamoto M, Kan S, Bando T, Goda H, Nakashiro K, Shimodaira S, Koido S, Homma S, Fujita T, Sato M, Yamashita N, Hamakawa H, Kawakami Y
  • Journal Title: Neoplasia Volume: 15(7) Pages: 805-14
  • Peer Reviewed
• [Journal Article] Targeting Aurora kinase A suppresses the growth of human oral squamous cell carcinoma cells in vitro and in vivo (2013)
  • Author(s): Tanaka H, Nakashiro K, Iwamoto K, Tokuzen N, Fujita Y, Shirakawa R, Oka R,Goda H, Hamakawa H
  • Journal Title: Oral Oncol Volume: 49(6) Pages: 551-9
  • Peer Reviewed
• [Journal Article] Prognostic impact of expression of bcl-2 and bax genes in circulating immune cells derived from patients with head and neck carcinoma (2013)
  • Author(s): Tano T, Okamoto M, Kan S, Nakashiro K, Shimodaira S, Koido S, Homma S, Sato M,Fujita T, Kawakami Y, Hamakawa H
  • Journal Title: Neoplasia Volume: 15(3) Pages: 305-14
  • Peer Reviewed
• [Journal Article] Targeting Aurora kinase A suppresses the growth of human oral squamous cell carcinoma cells in vitro and in vivo (2013)
  • Author(s): Tanaka H, Nakashiro K, Iwamoto K, Tokuzen N, Fujita Y, Shirakawa R, Oka R, Goda H, Hamakawa H
  • Journal Title: Oral Oncology Volume: 49 Issue: 6 Pages: 551-559
  • DOI: 10.1016/j.oraloncology.2013.02.002
  • Peer Reviewed
• [Journal Article] Premetastatic vasculogenesis in oral squamous cell carcinoma xenograft-draining lymph nodes (2012)
  • Author(s): Mayorca-Guiliani AE, Yano H, Nakashiro K, Hamakawa H, Tanaka J
  • Journal Title: Oral Oncol Volume: 48(8) Pages: 663-70
  • Peer Reviewed
• [Journal Article] Growth inhibition and apoptosis by an active component of OK-432, a streptococcal agent, via Toll-like receptor 4 in human head and neck cancer cell lines (2012)
  • Author(s): Tano T, Okamoto M, Kan S, Nakashiro K, Shimodaira S, Yamashita N, Kawakami Y, Hamakawa H
  • Journal Title: Oral Oncol Volume: 48(8) Pages: 678-85
  • Peer Reviewed
• [Journal Article] One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma (2012)
  • Author(s): Goda H
  • Journal Title: Oral Oncology Volume: 48 Issue: 10 Pages: 958-63
  • DOI: 10.1016/j.oraloncology.2012.03.026
  • Peer Reviewed
• [Presentation] 口腔扁平上皮癌におけるmRNA/microRNA発現の統合解析 (2014)
  • Author(s): 中城公一、岩本和樹、徳善紀彦、田中宏史、浜川裕之
  • Organizer: 第32回日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 札幌
• [Presentation] 口腔扁平上皮癌の転移を支持する分子基盤の解明 (2013)
  • Author(s): 中城公一、田中宏史、徳善紀彦、岩本和樹、日野聡史、浜川裕之
  • Organizer: 第50回日本口腔組織培養学会学術大会
  • Place of Presentation: 東京
• [Presentation] Identification of a novel tumor suppressive microRNA in human oral cancer cells (2013)
  • Author(s): Iwamoto K, Nakashiro K, Tanaka H, Oka R, Tokuzen N, Hamakawa H
  • Organizer: 21^<st> International Conference of Oral and Maxillofacial Surgery
  • Place of Presentation: Barcelona, Spain
• [Presentation] 口腔扁平上皮癌におけるmicroRNA-629の発現と機能 (2013)
  • Author(s): 岩本和樹、中城公一、田中宏史、徳善紀彦、岡亮太、浜川裕之
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 口腔扁平上皮癌センチネルリンパ節生検へのOSNA法応用の試み (2013)
  • Author(s): 中城公一、岩本和樹、岡亮太、合田啓之、浜川裕之
  • Organizer: 第15回SNNS研究会学術集会
  • Place of Presentation: 釧路
• [Presentation] 口腔悪性腫瘍に対する抗癌剤感受性試験の有用性 (2013)
  • Author(s): 中城公一、岩本和樹、岡亮太、田中宏史、浜川裕之
  • Organizer: 第67回日本口腔科学会学術集会
  • Place of Presentation: 宇都宮
• [Presentation] MicroRNA-133a/bのヒト口腔癌細胞の増殖における役割 (2013)
  • Author(s): 田中宏史、中城公一、岩本和樹、徳善紀彦、浜川裕之
  • Organizer: 第67回日本口腔科学会学術集会
  • Place of Presentation: 宇都宮
• [Presentation] ヒト口腔癌細胞におけるmicroRNA-629*の増殖抑制効果 (2012)
  • Author(s): 岩本和樹、中城公一、田中宏史、岡亮太、浜川裕之
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] ヒト口腔癌細胞における microRNA-629* の増殖抑制効果 (2012)
  • Author(s): 岩本和樹、中城公一、他
  • Organizer: 第 71 回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Patent(Industrial Property Rights)] 頭頸部癌の腫瘍マーカー (2013)
  • Inventor(s): 中城公一、浜川裕之
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2013-11-29
• [Patent(Industrial Property Rights)] Akt遺伝子に特異的なsiRNA (2013)
  • Inventor(s): 中城公一、浜川裕之
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2013-04-05
• [Patent(Industrial Property Rights)] ADAT1遺伝子に特異的なsiRNA (2012)
  • Inventor(s): 中城公一、浜川裕之
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2012-10-12
• [Patent(Industrial Property Rights)] 頭頸部癌の腫瘍マーカー (2012)
  • Inventor(s): 中城公一、浜川裕之
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2012-04-13
• [Patent(Industrial Property Rights)] アンドロゲン受容体遺伝子に特異的なsiRNA (2012)
  • Inventor(s): 中城公一、浜川裕之
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2012-04-06