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Development of a novel cancer vaccine that targets oral cancer stem cells

Research Project

Project/Area Number 24659901
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionSapporo Medical University

Principal Investigator

HIRATSUKA Hiroyoshi  札幌医科大学, 医学部, 教授 (50165180)

Co-Investigator(Kenkyū-buntansha) DEHARI Hironari  札幌医科大学, 医学部, 講師 (00381260)
MIYAZAKI Akihiro  札幌医科大学, 医学部, 准教授 (10305237)
OGI Kazuhiro  札幌医科大学, 医学部, 講師 (40433114)
MICHIFURI Yoshitaka  札幌医科大学, 医学部, 研究員 (00457722)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords口腔がん / がん幹細胞 / がんワクチン / ALDH1 / ワクチン / 幹細胞
Outline of Final Research Achievements

Oral cancer stem cells were isolated from oral cancer cell lines as aldehyde dehydrogenase 1 bright (ALDH1br) cells using the ALDEFLUOR assay. Expression of cancer stem cell-associated genes is increased in ALDH1br cells with a strong carcinogenesis and a high proliferative capacity. The small proline-rich protein-1B (SPRR1B) gene was shown to be overexpressed in ALDH1br cells. SPRR1B gene knockdown decreased the ALDH1br cell population and its proliferative capacity. Expression of the tumor suppressor gene Ras association domain family member 4 (RASSF4) was found to be suppressed in cells overexpressing SPRR1B. Our results suggest that oral cancer stem cells isolated as ALDH1br cells and SPRR1B have a role in cell growth by the suppression of RASSF4.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (28 results)

All 2014 2013 2012 Other

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (19 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Small proline-rich protein-1B is overexpressed in human oral squamous cell cancer stem-like cells and is related to their growth through activation of MAP kinase signal.2013

    • Author(s)
      Michifuri Y, Hirohashi Y, Torigoe T, Miyazaki A, Fujino J, Tamura Y, Tsukahara T, Kanaseki T, Kobayashi J, Sasaki T, Takahashi A, Nakamori K, Yamaguchi A, Hiratsuka H, Sato N.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 439 Issue: 1 Pages: 96-102

    • DOI

      10.1016/j.bbrc.2013.08.021

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Six-transmembrane epithelial antigen of the prostate-1 plays a role for in vivo tumor growth via intercellular communication2013

    • Author(s)
      Yamamoto T, Tamura Y, Kobayashi J, Kamiguchi K, Hirohashi Y, Miyazaki A, Torigoe T, Asanuma H, Hiratsuka H, Sato N
    • Journal Title

      Exp. Cell Res.

      Volume: 319 Issue: 17 Pages: 2617-29

    • DOI

      10.1016/j.yexcr.2013.07.025

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Silencing of GLUT-1 inhibits sensitization of oral cancer cells to cisplatin during hypoxia.2013

    • Author(s)
      Shimanishi M, Ogi K, Sogabe Y, Kaneko T, Dehari H, Miyazaki A, Hiratsuka H.
    • Journal Title

      J Oral Pathol Med.

      Volume: 42(5) Issue: 5 Pages: 382-8

    • DOI

      10.1111/jop.12028

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Highexpression of ALDH1 and SOX2 diffuse staining pattern of oral squamous cellcarcinomas correlates to lymph node metastasis.2013

    • Author(s)
      Michifuri Y, Hirohashi Y, Torigoe T,Miyazaki A, Kobayashi J, Sasaki T, FujinoJ, Asanuma H, Tamura Y, Nakamori K, Hasegawa T, Hiratsuka H, Sato N.
    • Journal Title

      Pathol Int.

      Volume: 62(10) Issue: 10 Pages: 684-9

    • DOI

      10.1111/j.1440-1827.2012.02851.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 口腔悪性腫瘍2012

    • Author(s)
      宮崎晃亘
    • Journal Title

      医学と薬学

      Volume: 67 Pages: 799-805

    • Related Report
      2012 Research-status Report
  • [Journal Article] General rules for clinical and pathological studies on oral cancer2012

    • Author(s)
      Izumo T
    • Journal Title

      Jpn J Clin Oncol.

      Volume: 42 Pages: 1099-1109

    • Related Report
      2012 Research-status Report
  • [Journal Article] Granulocyte colony-stimulating factor-producing squamous cellcarcinoma of the lower gingiva: a case report.2012

    • Author(s)
      Kobayashi J, Miyazaki A, Yamamot T, Nakamori K, Suzuki R, Kaneko T, Suzuki N, Hiratsuka H.
    • Journal Title

      Head Neck Oncol.

      Volume: 4 Issue: 1 Pages: 35-35

    • DOI

      10.1186/1758-3284-4-35

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Immunotherapeutic benefit of α-interferon in survivin2B-derived peptide vaccination for patients with oral cancer.2014

    • Author(s)
      Akihiro Miyazaki, Junichi Kobayashi, Toshihiko Torigoe, Yoshihiko Hirohashi, Takanori Sasaki, Sho Miyamoto, Kazuhiro Ogi, Hironari Dehari, Noriyuki Sato, Hiroyoshi Hiratsuka
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Tumor cells exposed to microenvironmental stresses express non-classical MHC Ib that attenuates CD8+ T cell responses2014

    • Author(s)
      Takanori Sasaki, Takayuki Kanaseki, Sachiyo Kawami, Eri Saka, Yasuaki Tamura, Toshihiko Torigoe, Hiroyoshi Hiratsuka, Noriyuki Sato
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] A next-Generation Sequencing Screen for Mutational Hotspots in 50 Cancer-Related Genes using Semiconductor Sequencer.2014

    • Author(s)
      Takafumi Nakagaki, Yasushi Sasaki, Masashi Idogawa, Kousuke Takeda, Miyuki Tamura, Tomoko Ohashi, Kazuhiro Ogi, Hiroyoshi Hiratsuka, Takashi Tokino
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of the natural antigenic peptide derived from FAM83B that is specifically expressed in cancer stem cells.2014

    • Author(s)
      Sho Miyamoto, Takayuki Kanaseki, Akari Takahashi, Kochin Vitaly, Yoshihiko Hirohashi, Toshihiko Torigoe, Hiroyoshi Hiratsuka, Noriyuki Sato
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of long non-coding RNAs potentially involved in oral squamous cell carcinoma.2014

    • Author(s)
      Koyo Nishiyama, Reo Maruyama, Kosuke Takeda, Takahumi Nakagaki, Takeshi Niinuma, Kazuhiro Ogi, Hironari Dehari, Akihiro Miyazaki, Masahiro Kai, Yasushi Sasaki, Takeshi Tokino, Hiromu Suzuki, Hiroyoshi Hiratsuka
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification and characterization of the intercellular adhesion molecule-2 gene as a novel p53 family target2014

    • Author(s)
      Kousuke Takeda,Yasushi Sasaki , Takafumi Nakagaki, Miyuki Tamura, Masashi Idogawa , Kazuhiro Ogi, Hiroyoshi Hiratsuka, Takashi Tokino
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Wnt/β-catenin signaling pathway directly controls hypoxia induced epithelial mesenchymal transition in oral cancer2014

    • Author(s)
      Takeshi Kaneko, Hironari Dehari, Kazuhiro Ogi, Kousuke Takeda, Junichi Kobayashi, Akihiro Miyazaki, Hiroyoshi Hiratsuka
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 口腔がんに対するサバイビン2Bペプチドワクチン・インターフェロン併用療法の臨床試験2014

    • Author(s)
      宮崎晃亘、小林淳一、鳥越俊彦、廣橋良彦、佐々木敬則、宮本昇、出張裕也、荻和弘、佐藤昇志、平塚博義
    • Organizer
      第52回日本癌治療学会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] StageI,II口腔扁平上皮癌の治療成績の検討2013

    • Author(s)
      五十嵐友彦
    • Organizer
      第37回日本頭頸部癌学会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 進行・再発口腔がんに対するアジュバント併用survivin-2Bペプチドワクチン療法2013

    • Author(s)
      宮崎晃亘
    • Organizer
      第37回日本頭頸部癌学会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 口腔癌に対するCDDPを用いた放射線併用選択的動注化学療法の検討2013

    • Author(s)
      小林淳一
    • Organizer
      第37回日本頭頸部癌学会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] Tumor cells exposed to microenvironmental stresses express non-classical MHC class Ib that attenuates CD8+ T cell responses2013

    • Author(s)
      Takanori Sasaki
    • Organizer
      第72回日本癌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] The Wnt/β-catenin signaling pathway directly controls hypoxia-induced epitelial-mesenchymal transition in oral cancer.2013

    • Author(s)
      Takeshi Kaneko
    • Organizer
      第72回日本癌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] Identification and characterization of the intercellular adhesion molecule-2 (ICAM2) gene as a novel p53 family target.2013

    • Author(s)
      Kousuke Takeda
    • Organizer
      第72回日本癌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] 口腔がんに対するアジュバント併用survivin-2Bペプチドワクチン療法の臨床・免疫学的効果2012

    • Author(s)
      宮崎晃亘
    • Organizer
      第66回 日本口腔科学会学術集会
    • Place of Presentation
      広島
    • Related Report
      2012 Research-status Report
  • [Presentation] 小胞体ストレス下で提示される新規MHCI抗原ペプチド群の解析2012

    • Author(s)
      佐々木敬則
    • Organizer
      第66回 日本口腔科学会学術集会
    • Place of Presentation
      広島
    • Related Report
      2012 Research-status Report
  • [Presentation] 口蓋に発生した多形腺腫由来筋上皮癌の1例2012

    • Author(s)
      竹田康佑
    • Organizer
      第66回 日本口腔科学会学術集会
    • Place of Presentation
      広島
    • Related Report
      2012 Research-status Report
  • [Presentation] Sliencing of GLUT1 inhibits sensitizes oral cancer cells to CDDP during hypixia2012

    • Author(s)
      荻 和弘
    • Organizer
      第71回 日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] Survivin-dirived peptide vaccine therapy for patients with advanced or recurrent oral cancer-Phase I clinical trial2012

    • Author(s)
      宮崎晃亘
    • Organizer
      第71回 日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Book] Tongue: Anatomy, Kinematics and Diseases2012

    • Author(s)
      Nakamori K
    • Total Pages
      233
    • Publisher
      Nova Science Publishers, Inc
    • Related Report
      2012 Research-status Report
  • [Remarks] 札幌医科大学医学部口腔外科学講座 悪性腫瘍

    • URL

      http://web.sapmed.ac.jp/oral/guide/special/m16l6f00000002fm.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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