| Project/Area Number |
24659901
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
DEHARI Hironari 札幌医科大学, 医学部, 講師 (00381260)
MIYAZAKI Akihiro 札幌医科大学, 医学部, 准教授 (10305237)
OGI Kazuhiro 札幌医科大学, 医学部, 講師 (40433114)
MICHIFURI Yoshitaka 札幌医科大学, 医学部, 研究員 (00457722)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 口腔がん / がん幹細胞 / がんワクチン / ALDH1 / ワクチン / 幹細胞 |
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| Outline of Final Research Achievements |
Oral cancer stem cells were isolated from oral cancer cell lines as aldehyde dehydrogenase 1 bright (ALDH1br) cells using the ALDEFLUOR assay. Expression of cancer stem cell-associated genes is increased in ALDH1br cells with a strong carcinogenesis and a high proliferative capacity. The small proline-rich protein-1B (SPRR1B) gene was shown to be overexpressed in ALDH1br cells. SPRR1B gene knockdown decreased the ALDH1br cell population and its proliferative capacity. Expression of the tumor suppressor gene Ras association domain family member 4 (RASSF4) was found to be suppressed in cells overexpressing SPRR1B. Our results suggest that oral cancer stem cells isolated as ALDH1br cells and SPRR1B have a role in cell growth by the suppression of RASSF4.
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