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Development of anti- leukotoxin expression reagent from Aggregatibacter actinomycetemcomitans by using antisense peptide nucleic acids.

Research Project

Project/Area Number 24659912
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthodontic/Pediatric dentistry
Research InstitutionNagasaki University

Principal Investigator

HOSHINO Tomonori  長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (00359960)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords若年性歯周炎 / A. actinomycetemcomitans / アンチセンスPNA / 膜透過型ペプチド / 細菌叢解析 / ロイコトキシン / lktA / Aa菌 / Aggregatibacter / actinomycetemcomitans
Outline of Final Research Achievements

We investigated whether the mRNA expression of lktA gene from Aggregatibacter actinomycetemcomitans, the causative bacteria of aggressive periodontitis, was suppressed by antisense peptide nucleic acids (PNA).
Antisense PNA, which has antisense sequence from initiation codon to 12th base of lktA gene, was composed and conjugated (KFF)3 as a cell penetrating peptides. 0 to 50 μM of antisense PNA was reacted to 3x10E8 cfu of A. actinomycetemcomitans at 37℃ in 5% CO2 for 1hrs and the mRNA expression of lktA gene was measured by real-time RT-PCR. As the result, 20 μM of antisense PNA could most inhibit among the concentration.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (3 results) (of which Invited: 1 results)

  • [Journal Article] 口腔ケアでは細菌学的には何を標的とするべきか? -脳性麻痺患者の唾液中細菌叢の群集解析などで得られた知見から-2015

    • Author(s)
      星野倫範
    • Journal Title

      日本口腔ケア学会誌

      Volume: 9 Pages: 5-11

    • NAID

      40020472778

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Taxonomy of the Anginosus group of the genus Streptococcus and description of Streptococcus anginosus subsp. whileyi subsp. nov. and Streptococcus constellatus subsp. viborgensis subsp. nov.2013

    • Author(s)
      Jensen Anders, Hoshino Tomonori, Kilian Mogens
    • Journal Title

      Int J Syst Evol Microbiol

      Volume: 63 Issue: Pt_7 Pages: 2506-2519

    • DOI

      10.1099/ijs.0.043232-0

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Streptococcus mutansグルコシルトランスフェラーゼBの抗原部位の解析と抗齲蝕ワクチン開発への展望2013

    • Author(s)
      星野 倫範
    • Journal Title

      小児歯科学雑誌

      Volume: 51 Pages: 317-325

    • NAID

      10031189866

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evolution of cariogenic character in Streptococcus mutans: horizontal transmission of glycosyl hydrolase family 70 genes.2012

    • Author(s)
      Hoshino T
    • Journal Title

      Scientific Reports

      Volume: 2 Issue: 1 Pages: 518-518

    • DOI

      10.1038/srep00518

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 口腔ケアにより何を変えなければならないか -群集解析による脳性麻痺群患者と健常者の口腔細菌叢の比較から-2014

    • Author(s)
      星野倫範、佐藤恭子、西俣はるか、釜崎陽子、西口美由季、藤原 卓
    • Organizer
      第11回日本口腔ケア学会総会学術大会
    • Place of Presentation
      旭川市民文化会館(北海道旭川市)
    • Year and Date
      2014-06-28 – 2014-06-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 当科母親教室を受診した8か月児の口腔内細菌叢2013

    • Author(s)
      西俣はるか、齋藤幹、星野倫範、日高聖、佐藤恭子、藤原 卓
    • Organizer
      第51回日本小児歯科学会総会
    • Place of Presentation
      岐阜 長良川国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] メタゲノム解析による脳性麻痺患者と健常者の口腔細菌叢の比較2013

    • Author(s)
      星野倫範、佐藤恭子、西俣はるか、釜崎陽子、西口美由季、藤原 卓
    • Organizer
      第30回日本障害者歯科学会
    • Place of Presentation
      神戸 神戸国際展示場
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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