| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Outline of Final Research Achievements |
We investigated whether the mRNA expression of lktA gene from Aggregatibacter actinomycetemcomitans, the causative bacteria of aggressive periodontitis, was suppressed by antisense peptide nucleic acids (PNA).
Antisense PNA, which has antisense sequence from initiation codon to 12th base of lktA gene, was composed and conjugated (KFF)3 as a cell penetrating peptides. 0 to 50 μM of antisense PNA was reacted to 3x10E8 cfu of A. actinomycetemcomitans at 37℃ in 5% CO2 for 1hrs and the mRNA expression of lktA gene was measured by real-time RT-PCR. As the result, 20 μM of antisense PNA could most inhibit among the concentration.
|
